Paraoxonase1, its Q192R polymorphism and HDL-cholesterol in relation to intensive cardiac care unit stay in ischemic heart disease

• Published in: Indian journal of human genetics Vol. 20; no. 1; pp. 51 - 58
• Main Authors: Hampe, MaheshHarishchandra, Mogarekar, MukundRamchandra
• Format: Journal Article
• Language: English
• Published: India Medknow Publications and Media Pvt. Ltd 2014; Medknow Publications & Media Pvt. Ltd; Medknow Publications & Media Pvt Ltd
• Subjects: Cardiovascular disease; Care and treatment; Cholesterol; Confidence intervals; Coronary heart disease; Diagnosis; Electrocardiography; Enzymes; Genetic aspects; Hydrolysis; Lipoproteins; Mortality; Original; Physiological aspects; Regression analysis; Risk factors; Single nucleotide polymorphisms; Statistical analysis; India; paraoxonase1; Coronary artery disease; paraoxonase1 Q192R polymorphism
• ISSN: 0971-6866; 1998-362X
• DOI: 10.4103/0971-6866.132756

The present study was evaluated the atheroprotective potential of paraoxonase1 (PON1) and its Q192R polymorphism, to determine whether this polymorphism, which is responsible for differential PON1 activity plays any role in the pathogenesis, severity and extent of coronary artery disease (CAD). This hospital-based cross-sectional study investigated 60 diagnosed cases of CAD and 60 age and gender matched controls. All were assessed for serum PON1 activity, PON1 Q192R polymorphism and for classical cardiovascular risk factors. Individual serum phenotyping for PON1 Q192R polymorphism was determined by double substrate hydrolysis assay. Severity of CAD was assessed by the length of intensive cardiac care unit (ICCU) stay. Serum PON1 activity is significantly reduced in cases of CAD (92.6 ± 31.13 IU/L when compared with controls (105.26 ± 32.53 IU/L). Furthermore, serum arylesterase activity is reduced in CAD patients (90.31 ± 23.26 kU) when compared with the control subjects (101.61 ± 28.68 kU). Serum PON1 and arylesterase activities are significantly negatively correlated with the length of ICCU stay (r = -393 and r = -374 respectively). There is no significant difference in the occurrence of CAD and length of ICCU stay among the PON1 phenotypes (P = 0.92). Logistic regression analysis after adjustment of established risk factors revealed no significant association between CAD risk and PON1 Q192R polymorphism (odds ratios: 1.179 [95% confidence intervals: 0.507-2.744], P = 0.702). The current study demonstrates that the activity of the PON1 enzyme may be more important factor than the PON1 Q192R polymorphism in the severity and extent of CAD.