Genetic diversity and hybridization of Pulsatilla tongkangensis based on the nrDNA ITS region sequence
Pulsatilla tongkangensis, a perennial medicinal herb growing on the cliffs of Tongkang, Kangwon Province, Korea, is accordingly named as P. tongkangensis as a new Pulsatilla species. Due to sharing similar morphological characters with other Pulsatilla species, how to accurately discriminate P. tong...
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Published in | Biológia Vol. 69; no. 1; pp. 24 - 31 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
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01.01.2014
Springer Vienna Versita |
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ISSN | 0006-3088 1336-9563 1336-9563 |
DOI | 10.2478/s11756-013-0284-1 |
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Abstract | Pulsatilla tongkangensis, a perennial medicinal herb growing on the cliffs of Tongkang, Kangwon Province, Korea, is accordingly named as P. tongkangensis as a new Pulsatilla species. Due to sharing similar morphological characters with other Pulsatilla species, how to accurately discriminate P. tongkangensis becomes a critical, urgently needed-solved problem. To understand the truth of P. tongkangensis derivation and the phylogenetic relationship in Pulsatilla, the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) as the most frequently used marker for distinguishing plant species was amplified from P. tongkangensis in this study. According to sequence alignment result, three P. tongkangensis-specific nucleotide sites were obtained to vary from other Pulsatilla species, particularly one at 417 bp showing completely homology among P. tongkangensis accessions and completely dissimilarity between P. tongkangensis and other Pulsatilla species. As known fungal contamination is common in herbal medicinal materials when improperly processed and stored, using universal primers of the ITS region could not only plant DNA but parasitic fungal DNA. Thus, to improve the amplification condition of the ITS region from P. tongkangensis and design plant-specific primer would the following focus of our study. This work will be useful for clearly understanding the phylogenetic relationship between P. tongkangensis and other species belonging to the genus Pulsatilla. |
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AbstractList | Pulsatilla tongkangensis
, a perennial medicinal herb growing on the cliffs of Tongkang, Kangwon Province, Korea, is accordingly named as
P. tongkangensis
as a new
Pulsatilla species
. Due to sharing similar morphological characters with other
Pulsatilla
species, how to accurately discriminate
P. tongkangensis
becomes a critical, urgently needed-solved problem. To understand the truth of
P. tongkangensis
derivation and the phylogenetic relationship in
Pulsatilla
, the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) as the most frequently used marker for distinguishing plant species was amplified from
P. tongkangensis
in this study. According to sequence alignment result, three
P. tongkangensis
-specific nucleotide sites were obtained to vary from other
Pulsatilla
species, particularly one at 417 bp showing completely homology among
P. tongkangensis
accessions and completely dissimilarity between
P. tongkangensis
and other
Pulsatilla
species. As known fungal contamination is common in herbal medicinal materials when improperly processed and stored, using universal primers of the ITS region could not only plant DNA but parasitic fungal DNA. Thus, to improve the amplification condition of the ITS region from
P. tongkangensis
and design plant-specific primer would the following focus of our study. This work will be useful for clearly understanding the phylogenetic relationship between
P. tongkangensis
and other species belonging to the genus
Pulsatilla
. Pulsatilla tongkangensis, a perennial medicinal herb growing on the cliffs of Tongkang, Kangwon Province, Korea, is accordingly named as P. tongkangensis as a new Pulsatilla species. Due to sharing similar morphological characters with other Pulsatilla species, how to accurately discriminate P. tongkangensis becomes a critical, urgently needed-solved problem. To understand the truth of P. tongkangensis derivation and the phylogenetic relationship in Pulsatilla, the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) as the most frequently used marker for distinguishing plant species was amplified from P. tongkangensis in this study. According to sequence alignment result, three P. tongkangensis-specific nucleotide sites were obtained to vary from other Pulsatilla species, particularly one at 417 bp showing completely homology among P. tongkangensis accessions and completely dissimilarity between P. tongkangensis and other Pulsatilla species. As known fungal contamination is common in herbal medicinal materials when improperly processed and stored, using universal primers of the ITS region could not only plant DNA but parasitic fungal DNA. Thus, to improve the amplification condition of the ITS region from P. tongkangensis and design plant-specific primer would the following focus of our study. This work will be useful for clearly understanding the phylogenetic relationship between P. tongkangensis and other species belonging to the genus Pulsatilla. |
Author | Sun, Yan-Lin Park, Wan-Geun Hong, Soon-Kwan Oh, Hyun-Kyung |
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Cites_doi | 10.1177/00912700022009206 10.1002/jssc.201000660 10.3732/ajb.89.10.1599 10.1111/j.1759-6831.2011.00137.x 10.3390/molecules15031891 10.1016/j.jep.2010.01.014 10.1073/pnas.0408302101 10.1201/9781439833278.ch14 10.1016/j.brainres.2007.09.084 10.1006/mpev.1997.0422 10.1016/S1055-7903(03)00194-5 10.1046/j.1365-294x.1998.00409.x 10.1016/j.jep.2004.05.016 |
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Snippet | Pulsatilla tongkangensis, a perennial medicinal herb growing on the cliffs of Tongkang, Kangwon Province, Korea, is accordingly named as P. tongkangensis as a... Pulsatilla tongkangensis , a perennial medicinal herb growing on the cliffs of Tongkang, Kangwon Province, Korea, is accordingly named as P. tongkangensis as a... |
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SubjectTerms | Biomedical and Life Sciences Cell Biology fungi genetic variation hybridization internal transcribed spacers Korean Peninsula Life Sciences medicinal plants Microbiology molecular identification nrDNA ITS phylogeny Plant Sciences Pulsatilla Pulsatilla tongkangensis ribosomal DNA Section Botany sequence alignment Zoology |
Title | Genetic diversity and hybridization of Pulsatilla tongkangensis based on the nrDNA ITS region sequence |
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