Identifying mRNAs Bound by Human RBMY Protein in the Testis
Rbmy gene encodes a germ-cell specific nuclear RNA-binding protein and is involved in spermatogenesis. To further investigate the specific events of spermatogenesis in which Rbmy plays a role, the target mRNAs of human RBMY protein were isolated and identified. Through the isolating specific nucleic...
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| Published in | Journal of Reproduction and Development Vol. 57; no. 1; pp. 107 - 112 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
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Japan
THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT
01.02.2011
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| Online Access | Get full text |
| ISSN | 0916-8818 1348-4400 |
| DOI | 10.1262/jrd.10-092N |
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| Abstract | Rbmy gene encodes a germ-cell specific nuclear RNA-binding protein and is involved in spermatogenesis. To further investigate the specific events of spermatogenesis in which Rbmy plays a role, the target mRNAs of human RBMY protein were isolated and identified. Through the isolating specific nucleic acids associated with proteins (SNAAP) technique, we isolated twenty potential target genes of human RBMY protein from the human testis in the present study. Some of these target genes play important roles during spermatogenesis and have alternative transcripts in the testis. In this study, we focused on the human- related (never in mitosis gene a) kinase 10 (Nek10) gene, which belongs to the Nek protein kinase subfamily. Nek10 has two transcripts, and the results of RT-PCR and Electrophoretic Mobility Shift Assays (EMSA) show that hRBMY protein can only bind to transcript variant 2 of Nek10 and that hRbmy may take part in alternative splicing of Nek10. Isolation and identification of target genes of hRBMY will be helpful to further investigate the biological function of RBMY in spermatogenesis. |
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| AbstractList | Rbmy gene encodes a germ-cell specific nuclear RNA-binding protein and is involved in spermatogenesis. To further investigate the specific events of spermatogenesis in which Rbmy plays a role, the target mRNAs of human RBMY protein were isolated and identified. Through the isolating specific nucleic acids associated with proteins (SNAAP) technique, we isolated twenty potential target genes of human RBMY protein from the human testis in the present study. Some of these target genes play important roles during spermatogenesis and have alternative transcripts in the testis. In this study, we focused on the human- related (never in mitosis gene a) kinase 10 (Nek10) gene, which belongs to the Nek protein kinase subfamily. Nek10 has two transcripts, and the results of RT-PCR and Electrophoretic Mobility Shift Assays (EMSA) show that hRBMY protein can only bind to transcript variant 2 of Nek10 and that hRbmy may take part in alternative splicing of Nek10. Isolation and identification of target genes of hRBMY will be helpful to further investigate the biological function of RBMY in spermatogenesis. |
| Author | ZENG, Mei LIU, Yanyan ZHANG, Sizhong SUN, Huaqin ZHAO, Shaozhi LIANG, Suhua MA, Yongxin |
| Author_xml | – sequence: 1 fullname: ZHANG, Sizhong organization: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University – sequence: 1 fullname: LIANG, Suhua organization: Biology Group of North Sichuan Medical College – sequence: 1 fullname: LIU, Yanyan organization: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University – sequence: 1 fullname: ZHAO, Shaozhi organization: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University – sequence: 1 fullname: MA, Yongxin organization: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University – sequence: 1 fullname: SUN, Huaqin organization: Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University – sequence: 1 fullname: ZENG, Mei organization: Biology Group of North Sichuan Medical College |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/21422736$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1007_s12038_012_9281_8 crossref_primary_10_1038_bjc_2015_408 crossref_primary_10_1186_s12958_018_0330_5 crossref_primary_10_1101_gad_291195_116 crossref_primary_10_1016_j_gene_2011_10_020 crossref_primary_10_1007_s10815_019_01492_z crossref_primary_10_1111_cas_14506 |
| Cites_doi | 10.1016/S0092-8674(00)81834-6 10.1016/S0070-2153(08)60174-X 10.1083/jcb.200701005 10.1007/BF00278879 10.1146/annurev.biochem.72.121801.161720 10.1093/molehr/gah037 10.1002/(SICI)1521-1878(199807)20:7<555::AID-BIES6>3.0.CO;2-J 10.1038/10279 10.1002/bies.10371 10.1093/nar/28.7.e20 10.1159/000015061 10.1111/j.1365-2605.2004.00496.x 10.1095/biolreprod66.2.475 10.1006/geno.1998.5255 10.1177/002215540305100916 10.1242/jcs.055889 10.1073/pnas.0505123103 10.1016/j.tibs.2005.07.001 10.1093/molehr/gan024 10.1371/journal.pgen.1000042 10.1093/nar/gki834 10.1016/0092-8674(93)90616-X 10.1073/pnas.97.11.5717 10.1093/nar/gkh979 10.1038/418236a 10.1017/S1355838299981803 10.1093/hmg/7.4.715 10.1016/S0079-6603(04)78002-2 10.1083/jcb.200709057 10.1038/nature01722 10.1002/dvg.1020160202 10.1016/0168-9525(87)90296-4 10.1038/ng0297-131 10.1093/hmg/ddn179 10.1093/hmg/5.7.869 10.1091/mbc.e05-04-0315 10.1038/sj.embor.7400910 |
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| References | 21. King PH. RNA-binding analyses of Huc and HuD with the VEGF and c-myc 3-untranslated regions using a novel ELISA-based assay. Nucleic Acids Res 2000; 28: E20. 17. Elliott DJ, Bourgeois CF, Klink A, Stevenin J, Cooke HJA. Mammalian germ cell-specific RNA-binding protein interacts with ubiquitously expressed proteins involved in splice site selection. Proc Nat Acad Sci USA 2000; 97: 5717-5722. 16. Bourgeois CF, Lejeune F, Stévenin J. Broad specificity of SR (serine/arginine) proteins in the regulation of alternative splicing of premessenger RNA. Prog Nucleic Acid Res Mol Bio 2004; 78: 37-88. 2. Hecht NB. The making of a spermatozoon: a molecular perspective. Dev Genet 1995; 16: 95-103. 7. Chai NN, Zhou HY, Hernandez J, Najmabadi H, Bhasin S, Yen PH. Structure and organization of the RBMY genes on the human Y chromosome: transposition and amplification of an ancestral autosomal hnRNPG gene. Genomics 1998; 49: 283-289. 1. Willison K, Ashworth A. Mammalian spermatogenic gene expression. Trends Genet 1987; 3: 351-355. 5. Tiepolo L, Zuffardi O. Localisation of factors controlling spermatogenesis in the non-fluorecent portion of the human Y chromosome long arm. Hum Genet 1976; 34: 119-124. 4. Eddy EM, O'Brien DA. Gene expression during mammalian meiosis. Curr Top Dev Biol 1998; 37: 141-200. 13. Mahadevaiah SK, Odorisio T, Elliott DJ, Rattigan A, Szot M, Laval SH. Mouse homologues of the human AZF candidate gene RBM are expressed in spermatogonia and spermatids, and map to a Y chromosome deletion interval associated with a high incidence of sperm abnormalities. Hum Mol Genet 1998; 7: 715-727. 36. Grizzi F, Chiriva IM, Franceschini B, Hemonat PL, Soda G, Lim SH. Immunolocalization of sperm protein 17 in human testis and ejaculated spermatozoa. J Histochem Cytochem 2003; 51: 1245-1248. 15. Elliott DJ. The role of potential splicing factors including RBMY, RBMX, hnRNPG-T and STAR proteins in spermatogenesis. Int J Androl 2004; 27: 328-334. 24. Adelman CA, Petrini JHJ. ZIP4H (TEX11) Deficiency in the mouse impairs meiotic double strand break repair and the regulation of crossing over. Plos Genet 2008; 4: e1000042. 28. Maniatis T, Tasic B. Alternative pre-mRNA splicing and proteome expansion in metazoan. Nature 2002; 418: 236-243. 23. Yang F, Eckardt S, Leu NA, McLaughlin KJ, Wang PJ. Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis. J Cell Biol 2008; 180: 673-679. 32. Kan Z, Garrett-Engele PW, Johnson JM, Castle JC. Evolutionarily conserved and diverged alternative splicing events show different expression and functional profiles. Nucleic Acids Res 2005; 33: 5659-5666. 37. Skrisovska L, Bourgeois CF, Stefl R, Grellscheid SN, Kister L, Wenter P. The testis-specific human protein RBMY recognizes RNA through a novel mode of interaction. EMBO Reports 2007; 8: 372-379. 9. Delbridge ML, Disteche CM, Graves JAM, Lingenfelte PA. The candidate spermatogenesis gene RBMY has a homologue on the human X chromosome. Nat Genet 1999; 22: 223-224. 8. Skaletsky H, Kuroda-Kawaguchi T, Minx PJ, Cordum HS, Hillier L, Brown LG. The male-specific region of the human Y chromosome is a mosaic of discrete sequence classes. Nature 2003; 423: 825-837. 12. Delbridge ML, Harry JL, Toder R, Ma K, Chandley AC, Graves JAM. A human candidate spermatogenesis gene, RBM1, is conserved and amplified on the marsupial Y chromosome. Nat Genet 1997; 15: 131-136. 6. Ma K, Sharkey A, Bichmore WA, Hill RE, Prosser EJ, Speed RM. A Y chromosome gene family with RNA-binding protein homology: candidates for the azoospermia factor AZF controlling human spermatogenesis. Cell 1993; 75: 1287-1295. 31. Boue S, Letunic I, Bork P. Alternative splicing and evolution. Bioessays 2003; 25: 1031-1034. 20. Jiao X, Triffilis P, Kiledjian M. Identification of target messenger RNA substrates for the murine deleted in azoospermia-like RNA-binding protein. Biol Reprod 2002; 66: 475-485. 18. Trifillis P, Kiledjian M, Day N. Finding the right RNA: identification of cellular mRNA substrates for RNA-binding proteins. RNA 1999; 5: 1071-1082. 11. Elliott DJ, Ma K, Kerr SM, Thakrar R, Speed R, Chandley AC. An RBM homologue maps to the mouse Y chromosome and is expressed in germ cell. Hum Mol Genet 1996; 5: 869-874. 25. Maruyama O, Nishimor H, Kataqiri T, Miki Y, Ueno A, Nakamura Y. Cloning of TCFL5 encoding a novel human basic helix-loop-helix motif protein that is specifically expressed in primary spermatocytes at the pachytene stage. Cytogenet Cell Genet 1998; 82: 41-45. 22. Mei Z, Sun HQ, Chen S, Xinyang W, Yuan Y, Yunqiang L. Identification of target messenger RNA substrates for mouse RBMY. Mol Hum Reprod 2008; 14: 331-336. 14. Jungmin L, Jinpyo H, Euisu K, Kyungjin K, Soo WK. Developmental stage-specific expression of RBM suggests its involvement in early phase of spematogenesis. Mol Hum Reprod 2004; 10: 259-264. 27. Greenbaum MP, Yan W, Wu MH. TEX14 is essential for intercellular bridges and fertility in male mice. Proc Nat Acad Sci USA 2006; 103: 4982-4987. 35. Hecht NB. Molecular mechanisms of male germ cell differentiation. BioEssays 1998; 20: 555-561. 3. Sassone-Corsi P. Transcriptional checkpoints determining the fate of male germ cell. Cell 1997; 88: 163-166. 26. Siep M, Sleddens-Linkels E, Mulders S, Van EH, Wassenaar E, Van CWA. Basic helix-loop-helix transcription factor Tcfl5 interacts with the Calmegin gene promoter in mouse spermatogenesis. Nucleic Acids Res 2004; 32: 6425-6436. 30. Stetefeld J, Ruegg MA. Structural and functional diversity generated by alternative mRNA splicing. Trends Biochem Sci 2005; 30: 515-521. 34. Natacha D, Cyril FB, Fabrice L, Yilei L, Ingrid EE, David JE, James S. Human RBMY regulates germline-specific splicing events by modulating the function of the serine/arginine-rich proteins 9G8 and Tra2-β. J Cell Sci 2010; 123: 40-50. 10. Ehrmann I, Dalgliesh C, Tsaousi A, Paronetto MP, Heinrich B, Kist R. Haploinsufficiency of the germ cell-specific nuclear RNA binding protein hnRNP G-T prevents functional spermatogenesis in the mouse. Hum Mol Genet 2008; 17: 2803-2818. 29. Black DL. Mechanism of alternative pre-messenger RNA splicing. Annu Rev Biochem 2003; 72: 291-336. 33. Joan R, Aaron G, Jennifer C, Joseph A. Active Nerc2 protein kinase concentrates at centrosomes early in mitosis and is necessary for proper spindle assembly. Mol Bio Cell 2005; 16: 4827-4840. 19. Paronetto MP, Achsel T, Massiello A, Chalfant CE, Sette C. The RNA-binding protein Sam68 modulates the alternative splicing of Bcl-x. J Cell Biol 2007; 7: 929-939. 22 23 24 MARUYAMA O (25) 1998; 82 26 27 28 29 30 31 10 32 11 33 12 34 13 35 14 36 15 37 16 17 18 19 1 2 3 4 5 6 7 8 9 20 21 |
| References_xml | – reference: 22. Mei Z, Sun HQ, Chen S, Xinyang W, Yuan Y, Yunqiang L. Identification of target messenger RNA substrates for mouse RBMY. Mol Hum Reprod 2008; 14: 331-336. – reference: 9. Delbridge ML, Disteche CM, Graves JAM, Lingenfelte PA. The candidate spermatogenesis gene RBMY has a homologue on the human X chromosome. Nat Genet 1999; 22: 223-224. – reference: 37. Skrisovska L, Bourgeois CF, Stefl R, Grellscheid SN, Kister L, Wenter P. The testis-specific human protein RBMY recognizes RNA through a novel mode of interaction. EMBO Reports 2007; 8: 372-379. – reference: 29. Black DL. Mechanism of alternative pre-messenger RNA splicing. Annu Rev Biochem 2003; 72: 291-336. – reference: 24. Adelman CA, Petrini JHJ. ZIP4H (TEX11) Deficiency in the mouse impairs meiotic double strand break repair and the regulation of crossing over. Plos Genet 2008; 4: e1000042. – reference: 4. Eddy EM, O'Brien DA. Gene expression during mammalian meiosis. Curr Top Dev Biol 1998; 37: 141-200. – reference: 11. Elliott DJ, Ma K, Kerr SM, Thakrar R, Speed R, Chandley AC. An RBM homologue maps to the mouse Y chromosome and is expressed in germ cell. Hum Mol Genet 1996; 5: 869-874. – reference: 21. King PH. RNA-binding analyses of Huc and HuD with the VEGF and c-myc 3-untranslated regions using a novel ELISA-based assay. Nucleic Acids Res 2000; 28: E20. – reference: 23. Yang F, Eckardt S, Leu NA, McLaughlin KJ, Wang PJ. Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis. J Cell Biol 2008; 180: 673-679. – reference: 10. Ehrmann I, Dalgliesh C, Tsaousi A, Paronetto MP, Heinrich B, Kist R. Haploinsufficiency of the germ cell-specific nuclear RNA binding protein hnRNP G-T prevents functional spermatogenesis in the mouse. Hum Mol Genet 2008; 17: 2803-2818. – reference: 7. Chai NN, Zhou HY, Hernandez J, Najmabadi H, Bhasin S, Yen PH. Structure and organization of the RBMY genes on the human Y chromosome: transposition and amplification of an ancestral autosomal hnRNPG gene. Genomics 1998; 49: 283-289. – reference: 34. Natacha D, Cyril FB, Fabrice L, Yilei L, Ingrid EE, David JE, James S. Human RBMY regulates germline-specific splicing events by modulating the function of the serine/arginine-rich proteins 9G8 and Tra2-β. J Cell Sci 2010; 123: 40-50. – reference: 12. Delbridge ML, Harry JL, Toder R, Ma K, Chandley AC, Graves JAM. A human candidate spermatogenesis gene, RBM1, is conserved and amplified on the marsupial Y chromosome. Nat Genet 1997; 15: 131-136. – reference: 26. Siep M, Sleddens-Linkels E, Mulders S, Van EH, Wassenaar E, Van CWA. Basic helix-loop-helix transcription factor Tcfl5 interacts with the Calmegin gene promoter in mouse spermatogenesis. Nucleic Acids Res 2004; 32: 6425-6436. – reference: 13. Mahadevaiah SK, Odorisio T, Elliott DJ, Rattigan A, Szot M, Laval SH. Mouse homologues of the human AZF candidate gene RBM are expressed in spermatogonia and spermatids, and map to a Y chromosome deletion interval associated with a high incidence of sperm abnormalities. Hum Mol Genet 1998; 7: 715-727. – reference: 33. Joan R, Aaron G, Jennifer C, Joseph A. Active Nerc2 protein kinase concentrates at centrosomes early in mitosis and is necessary for proper spindle assembly. Mol Bio Cell 2005; 16: 4827-4840. – reference: 36. Grizzi F, Chiriva IM, Franceschini B, Hemonat PL, Soda G, Lim SH. Immunolocalization of sperm protein 17 in human testis and ejaculated spermatozoa. J Histochem Cytochem 2003; 51: 1245-1248. – reference: 28. Maniatis T, Tasic B. Alternative pre-mRNA splicing and proteome expansion in metazoan. Nature 2002; 418: 236-243. – reference: 17. Elliott DJ, Bourgeois CF, Klink A, Stevenin J, Cooke HJA. Mammalian germ cell-specific RNA-binding protein interacts with ubiquitously expressed proteins involved in splice site selection. Proc Nat Acad Sci USA 2000; 97: 5717-5722. – reference: 35. Hecht NB. Molecular mechanisms of male germ cell differentiation. BioEssays 1998; 20: 555-561. – reference: 6. Ma K, Sharkey A, Bichmore WA, Hill RE, Prosser EJ, Speed RM. A Y chromosome gene family with RNA-binding protein homology: candidates for the azoospermia factor AZF controlling human spermatogenesis. Cell 1993; 75: 1287-1295. – reference: 16. Bourgeois CF, Lejeune F, Stévenin J. Broad specificity of SR (serine/arginine) proteins in the regulation of alternative splicing of premessenger RNA. Prog Nucleic Acid Res Mol Bio 2004; 78: 37-88. – reference: 25. Maruyama O, Nishimor H, Kataqiri T, Miki Y, Ueno A, Nakamura Y. Cloning of TCFL5 encoding a novel human basic helix-loop-helix motif protein that is specifically expressed in primary spermatocytes at the pachytene stage. Cytogenet Cell Genet 1998; 82: 41-45. – reference: 20. Jiao X, Triffilis P, Kiledjian M. Identification of target messenger RNA substrates for the murine deleted in azoospermia-like RNA-binding protein. Biol Reprod 2002; 66: 475-485. – reference: 2. Hecht NB. The making of a spermatozoon: a molecular perspective. Dev Genet 1995; 16: 95-103. – reference: 8. Skaletsky H, Kuroda-Kawaguchi T, Minx PJ, Cordum HS, Hillier L, Brown LG. The male-specific region of the human Y chromosome is a mosaic of discrete sequence classes. Nature 2003; 423: 825-837. – reference: 19. Paronetto MP, Achsel T, Massiello A, Chalfant CE, Sette C. The RNA-binding protein Sam68 modulates the alternative splicing of Bcl-x. J Cell Biol 2007; 7: 929-939. – reference: 1. Willison K, Ashworth A. Mammalian spermatogenic gene expression. Trends Genet 1987; 3: 351-355. – reference: 15. Elliott DJ. The role of potential splicing factors including RBMY, RBMX, hnRNPG-T and STAR proteins in spermatogenesis. Int J Androl 2004; 27: 328-334. – reference: 14. Jungmin L, Jinpyo H, Euisu K, Kyungjin K, Soo WK. Developmental stage-specific expression of RBM suggests its involvement in early phase of spematogenesis. Mol Hum Reprod 2004; 10: 259-264. – reference: 30. Stetefeld J, Ruegg MA. Structural and functional diversity generated by alternative mRNA splicing. Trends Biochem Sci 2005; 30: 515-521. – reference: 31. Boue S, Letunic I, Bork P. Alternative splicing and evolution. Bioessays 2003; 25: 1031-1034. – reference: 18. Trifillis P, Kiledjian M, Day N. Finding the right RNA: identification of cellular mRNA substrates for RNA-binding proteins. RNA 1999; 5: 1071-1082. – reference: 5. Tiepolo L, Zuffardi O. Localisation of factors controlling spermatogenesis in the non-fluorecent portion of the human Y chromosome long arm. Hum Genet 1976; 34: 119-124. – reference: 3. Sassone-Corsi P. Transcriptional checkpoints determining the fate of male germ cell. Cell 1997; 88: 163-166. – reference: 27. Greenbaum MP, Yan W, Wu MH. TEX14 is essential for intercellular bridges and fertility in male mice. Proc Nat Acad Sci USA 2006; 103: 4982-4987. – reference: 32. Kan Z, Garrett-Engele PW, Johnson JM, Castle JC. Evolutionarily conserved and diverged alternative splicing events show different expression and functional profiles. Nucleic Acids Res 2005; 33: 5659-5666. – ident: 3 doi: 10.1016/S0092-8674(00)81834-6 – ident: 4 doi: 10.1016/S0070-2153(08)60174-X – ident: 19 doi: 10.1083/jcb.200701005 – ident: 5 doi: 10.1007/BF00278879 – ident: 29 doi: 10.1146/annurev.biochem.72.121801.161720 – ident: 14 doi: 10.1093/molehr/gah037 – ident: 35 doi: 10.1002/(SICI)1521-1878(199807)20:7<555::AID-BIES6>3.0.CO;2-J – ident: 9 doi: 10.1038/10279 – ident: 31 doi: 10.1002/bies.10371 – ident: 21 doi: 10.1093/nar/28.7.e20 – volume: 82 start-page: 41 issn: 0301-0171 issue: 1/2 year: 1998 ident: 25 publication-title: Cytogenet Cell Genet doi: 10.1159/000015061 – ident: 15 doi: 10.1111/j.1365-2605.2004.00496.x – ident: 20 doi: 10.1095/biolreprod66.2.475 – ident: 7 doi: 10.1006/geno.1998.5255 – ident: 36 doi: 10.1177/002215540305100916 – ident: 34 doi: 10.1242/jcs.055889 – ident: 27 doi: 10.1073/pnas.0505123103 – ident: 30 doi: 10.1016/j.tibs.2005.07.001 – ident: 22 doi: 10.1093/molehr/gan024 – ident: 24 doi: 10.1371/journal.pgen.1000042 – ident: 32 doi: 10.1093/nar/gki834 – ident: 6 doi: 10.1016/0092-8674(93)90616-X – ident: 17 doi: 10.1073/pnas.97.11.5717 – ident: 26 doi: 10.1093/nar/gkh979 – ident: 28 doi: 10.1038/418236a – ident: 18 doi: 10.1017/S1355838299981803 – ident: 13 doi: 10.1093/hmg/7.4.715 – ident: 16 doi: 10.1016/S0079-6603(04)78002-2 – ident: 23 doi: 10.1083/jcb.200709057 – ident: 8 doi: 10.1038/nature01722 – ident: 2 doi: 10.1002/dvg.1020160202 – ident: 1 doi: 10.1016/0168-9525(87)90296-4 – ident: 12 doi: 10.1038/ng0297-131 – ident: 10 doi: 10.1093/hmg/ddn179 – ident: 11 doi: 10.1093/hmg/5.7.869 – ident: 33 doi: 10.1091/mbc.e05-04-0315 – ident: 37 doi: 10.1038/sj.embor.7400910 |
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| Snippet | Rbmy gene encodes a germ-cell specific nuclear RNA-binding protein and is involved in spermatogenesis. To further investigate the specific events of... |
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| SubjectTerms | Alternative Splicing Electrophoretic Mobility Shift Assay Gene Expression Profiling Humans Male Nek10 NIMA-Related Kinases Nuclear Proteins - metabolism Protein Binding Protein-Serine-Threonine Kinases - genetics Protein-Serine-Threonine Kinases - metabolism Rbmy gene Recombinant Fusion Proteins - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - isolation & purification RNA, Messenger - metabolism RNA-binding protein RNA-Binding Proteins - metabolism SNAAP technique Spermatogenesis Testis - metabolism |
| Title | Identifying mRNAs Bound by Human RBMY Protein in the Testis |
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