Micropropagation and Shoot Tip Cryopreservation of ‘Sunny Gold’ Freesia

Cryopreservation is a promising method for the long-term preservation of plant germplasm, especially for vegetatively propagated species like freesias. In this study, we investigate streamlining the cryopreservation process for ‘Sunny Gold’ Freesia, starting from effective in vitro initiation and pr...

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Published inPlants (Basel) Vol. 13; no. 12; p. 1655
Main Authors Bae, Jinjoo, Song, Jae-Young, Lee, Young-Yi, Lee, Ye-ji, Choi, Youn Jung, Kwon, Oh-Keun, Nam, Sung-Hee, Lee, Ho-sun, Kim, Seok Cheol, Han, Ji-Won
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 14.06.2024
MDPI
Subjects
Benzyladenine
clonal preservation
Communication
Cryopreservation
Cultivars
droplet vitrification
Experiments
Freesia
Germplasm
Gold
Growth regulators
Horticulture
in vitro culture
Kinetin
Micropropagation
plant germplasm
Plant growth
plant growth regulators
plant growth substances
Regrowth
shoot tips
Shoots
species
Sterilization
Subculture
Sucrose
Tips
vegetative propagation
plant growth regulators
droplet vitrification
in vitro culture
clonal preservation
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ISSN2223-7747
2223-7747
DOI10.3390/plants13121655

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Summary:Cryopreservation is a promising method for the long-term preservation of plant germplasm, especially for vegetatively propagated species like freesias. In this study, we investigate streamlining the cryopreservation process for ‘Sunny Gold’ Freesia, starting from effective in vitro initiation and proliferation using various plant growth regulator combinations. We also assess the impact of subculture on regrowth rates after cryopreservation. The shoot tips were successfully initiated in vitro after sterilization. The shoots were multiplied an average of three times in media containing N6-benzyladenine and kinetin. The regrowth rates of non-cryopreserved shoot tips excised from different subculture cycles did not differ significantly, with rates of 44% observed for plants from more than five subcultures and 47% for those from three subcultures. However, only the shoot tips excised from cultures subjected to three subculture cycles were able to recover after cryopreservation, with a regrowth rate of 31%. Our findings lay the groundwork for the development of an efficient cryopreservation protocol for freesias in the future.
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ISSN:2223-7747
2223-7747
DOI:10.3390/plants13121655