Circulating B7-H3(CD276) Elevations in Cerebrospinal Fluid and Plasma of Children with Bacterial Meningitis

• Published in: Journal of molecular neuroscience Vol. 37; no. 1; pp. 86 - 94
• Main Authors: Chen, Xuqin, Zhang, Guangbo, li, Yan, Feng, Xing, Wan, Fengguo, Zhang, Liya, Wang, Jian, Zhang, Xueguang
• Format: Journal Article
• Language: English
• Published: New York Humana Press Inc 01.01.2009; Springer Nature B.V
• Subjects: Antigens, CD - blood; Antigens, CD - cerebrospinal fluid; Aseptic meningitis; B7 Antigens; Bacteria; Biomarkers - blood; Biomarkers - cerebrospinal fluid; Biomedical and Life Sciences; Biomedicine; Cell Biology; Central nervous system; Cerebrospinal fluid; Child; Child, Preschool; Children; Chronology; Clinical significance; Cytokines; Enzyme-linked immunosorbent assay; Female; Humans; Infant; Inflammation; Interferon; Interferon-gamma - blood; Interferon-gamma - cerebrospinal fluid; Interleukin 17; Interleukin-17 - blood; Interleukin-17 - cerebrospinal fluid; Interleukins; Leukocytes; Male; Meningitis; Meningitis, Bacterial - blood; Meningitis, Bacterial - cerebrospinal fluid; Meningitis, Bacterial - diagnosis; Neurochemistry; Neurology; Neurosciences; Plasma; Plasma levels; Proteomics; Receptors, Immunologic - blood; Tumor Necrosis Factor-alpha - blood; Tumor Necrosis Factor-alpha - cerebrospinal fluid; Tumor necrosis factor-TNF; Tumor necrosis factor-α; γ-Interferon; B7-H3; Plasma; Meningitis; Cerebrospinal fluid (CSF); Children
• ISSN: 0895-8696; 1559-1166; 1559-1166
• DOI: 10.1007/s12031-008-9133-z

The objective of this study was to analyze the clinical significance of cerebrospinal fluid (CSF) and plasma concentrations of B7-H3, tumor necrosis factor-alpha (TNF-α), gamma interferon (IFN-γ), and interleukin-17 (IL-17) in bacterial and aseptic meningitis in children. The participants were six children with bacterial meningitis, 16 with aseptic meningitis, and 12 control subjects. All participants were between 2 months and 12 years of age on admission. Cytokines determination was performed by enzyme-linked immunosorbent assay technique. CSF and plasma-circulating B7-H3 were significantly higher in the bacterial meningitis group as compared with the aseptic group ( p  = 0.001) and the control group ( p  = 0.000 and p  = 0.001 respectively). However, CSF and plasma-circulating B7-H3 in aseptic meningitis were not significantly higher than control group ( p  = 0.071 and p  = 0.72 respectively).CSF and plasma-circulating TNF-α were significantly higher in the bacterial meningitis group as compared with the aseptic group ( p  = 0.004 and p  < 0.0001 respectively) and control group ( p  = 0.004 and p  < 0.0001 respectively). Similarly, we did not observe significant elevated TNF-α levels in CSF and plasma in aseptic group compared with control group ( p  = 0.03 and p  = 0.12 respectively). IFN-γ levels in CSF and plasma were undetectable in control group, and we did not find statistical significances in both of CSF and plasma between the elevated IFN-γ level in bacterial meningitis group and aseptic meningitis group( p  = 0.055 and p  = 0.095 respectively) CSF and plasma levels of IL-17 were undetectable in all subjects. There were correlations between B7-H3 and TNF-α, IFN-γ ( r  = 0.875, p  = 0.000; r  = −0.693, p  = 0.000, respectively) in CSF in meningitis subjects. In plasma, levels of B7-H3 in bacterial meningitis on admission correlated positively with TNF-α ( r  = 0.968, p  = 0.002), and white blood cell counts ( r  = 0.973, p  = 0.001). Detectable CSF levels of B7-H3, TNF-α, and IFN-γ on admission were not associated significantly with any of CSF characteristics. Additionally, CSF and plasma levels of B7-H3 decreased remarkably after treatment. Altogether, our data indicated that circulating B7-H3 and TNF-α levels in the CSF and plasma were useful markers for distinguishing bacterial from aseptic meningitis, and Circulating B7-H3 was demonstrated to be useful in evaluating the intensity of the infectious inflammatory process in the central nervous system in children.