Changes in Mucin Production in Human Airway Epithelial Cells After Exposure to Electronic Cigarette Vapor With or Without Nicotine

• Published in: Clinical and experimental otorhinolaryngology Vol. 14; no. 3; pp. 303 - 311
• Main Authors: Song, Si-Youn, Kwak, So Young, Choi, Yoon Seok, Bae, Chang Hoon, Kim, Yong-Dae
• Format: Journal Article
• Language: English
• Published: Korean Society of Otorhinolaryngology-Head and Neck Surgery 01.08.2021; 대한이비인후과학회
• Subjects: e-cigarette vapor; epithelial cell; mitogen-activated protein kinase; muc5ac; nf-kappa b; Original; 이비인후과학
• ISSN: 1976-8710; 2005-0720; 2005-0720
• DOI: 10.21053/ceo.2020.01907

Objectives. The emergence of electronic cigarettes (e-cigarettes) has created new perceptions of the tobacco market. Unlike traditional tobacco, the greatest advantage of e-cigarettes is that they have less smell and are convenient and inexpensive. Most e-cigarette smokers believe that e-cigarette smoking is less harmful than traditional smoking. Information on the effects of e-cigarettes on human health is limited, and the issue remains controversial.Methods. We studied the effects of e-cigarette vapor on mucin (MUC5AC and MUC5B) and the change of MUC5AC and MUC5B from e-cigarette liquid with or without nicotine in respiratory epithelial cells. The effects of e-cigarette vapor with or without nicotine on mucin, along with the involved signaling pathways, were investigated using reverse transcriptase-polymerase chain reaction (PCR), real-time PCR, enzyme immunoassays, and immunoblot analysis with several specific inhibitors and small interfering RNA.Results. E-cigarette vapor with or without nicotine stimulated MUC5AC, but not MUC5B, expression in respiratory epithelial cells. In addition, we showed that e-cigarette vapor with and without nicotine induced MUC5AC expression via activation of the mitogen-activated protein kinase (MAPK; extracellular signal-regulated kinase [ERK] 1/2 and p38) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathways in human airway epithelial cells.Conclusion. E-cigarette vapor with and with nicotine significantly increased MUC5AC expression in human airway epithelial cells.