A new model of rectal cancer with regional lymph node metastasis allowing in vivo evaluation by imaging biomarkers
The work is aimed to develop a murine model of rectal cancer, which could be used to monitor lymph node metastasis development by magnetic resonance imaging (MRI) and optical imaging (OI) techniques. Ht-29 cancer cells were directly injected into the submucosal layer of the rectum of athymic nude mi...
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| Published in | Biomedicine & pharmacotherapy Vol. 65; no. 6; pp. 401 - 406 |
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| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
France
Elsevier SAS
01.09.2011
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0753-3322 1950-6007 1950-6007 |
| DOI | 10.1016/j.biopha.2011.04.027 |
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| Abstract | The work is aimed to develop a murine model of rectal cancer, which could be used to monitor lymph node metastasis development by magnetic resonance imaging (MRI) and optical imaging (OI) techniques.
Ht-29 cancer cells were directly injected into the submucosal layer of the rectum of athymic nude mice using trans-anal rectal cancer cell injection (TARCI). Thirty-six mice were inoculated with 10
×
10
5 cells and five mice were treated with sterile phosphate buffer solution. One to 4 weeks after cell injection, tumor growth was evaluated
in vivo using T2-weighted MRI at 4.7T. A further group of animal (
n
=
6) treated with ht-29_luc cells, with the same protocol, was monitored by optical imaging. In both groups, the presence of the primary tumor and of lymph nodes metastasis was confirmed by histology.
In all animals, primary tumors were detectable by MRI, 1 week from TARCI. After 4 weeks primary tumors showed a mean longitudinal diameter of about 2
cm. All animals developed regional lymph node metastases. Others organs (e.g. lung or liver) were not affected. In fat-suppressed, T2-weighted MRI, lymph nodes appeared as small areas characterized by hyper-intense signal compared to muscle. OI permitted evaluation of the primary tumor growth in perineal region.
TARCI of ht-29 cells into the rectum of nude mice is a feasible way to obtain a easily reproducible model of regional lymph node metastases could be monitored by magnetic resonance and optical imaging techniques. |
|---|---|
| AbstractList | The work is aimed to develop a murine model of rectal cancer, which could be used to monitor lymph node metastasis development by magnetic resonance imaging (MRI) and optical imaging (OI) techniques.
Ht-29 cancer cells were directly injected into the submucosal layer of the rectum of athymic nude mice using trans-anal rectal cancer cell injection (TARCI). Thirty-six mice were inoculated with 10×10(5) cells and five mice were treated with sterile phosphate buffer solution. One to 4 weeks after cell injection, tumor growth was evaluated in vivo using T2-weighted MRI at 4.7T. A further group of animal (n=6) treated with ht-29_luc cells, with the same protocol, was monitored by optical imaging. In both groups, the presence of the primary tumor and of lymph nodes metastasis was confirmed by histology.
In all animals, primary tumors were detectable by MRI, 1 week from TARCI. After 4 weeks primary tumors showed a mean longitudinal diameter of about 2cm. All animals developed regional lymph node metastases. Others organs (e.g. lung or liver) were not affected. In fat-suppressed, T2-weighted MRI, lymph nodes appeared as small areas characterized by hyper-intense signal compared to muscle. OI permitted evaluation of the primary tumor growth in perineal region.
TARCI of ht-29 cells into the rectum of nude mice is a feasible way to obtain a easily reproducible model of regional lymph node metastases could be monitored by magnetic resonance and optical imaging techniques. The work is aimed to develop a murine model of rectal cancer, which could be used to monitor lymph node metastasis development by magnetic resonance imaging (MRI) and optical imaging (OI) techniques. Ht-29 cancer cells were directly injected into the submucosal layer of the rectum of athymic nude mice using trans-anal rectal cancer cell injection (TARCI). Thirty-six mice were inoculated with 10 × 10 5 cells and five mice were treated with sterile phosphate buffer solution. One to 4 weeks after cell injection, tumor growth was evaluated in vivo using T2-weighted MRI at 4.7T. A further group of animal ( n = 6) treated with ht-29_luc cells, with the same protocol, was monitored by optical imaging. In both groups, the presence of the primary tumor and of lymph nodes metastasis was confirmed by histology. In all animals, primary tumors were detectable by MRI, 1 week from TARCI. After 4 weeks primary tumors showed a mean longitudinal diameter of about 2 cm. All animals developed regional lymph node metastases. Others organs (e.g. lung or liver) were not affected. In fat-suppressed, T2-weighted MRI, lymph nodes appeared as small areas characterized by hyper-intense signal compared to muscle. OI permitted evaluation of the primary tumor growth in perineal region. TARCI of ht-29 cells into the rectum of nude mice is a feasible way to obtain a easily reproducible model of regional lymph node metastases could be monitored by magnetic resonance and optical imaging techniques. The work is aimed to develop a murine model of rectal cancer, which could be used to monitor lymph node metastasis development by magnetic resonance imaging (MRI) and optical imaging (OI) techniques.OBJECTThe work is aimed to develop a murine model of rectal cancer, which could be used to monitor lymph node metastasis development by magnetic resonance imaging (MRI) and optical imaging (OI) techniques.Ht-29 cancer cells were directly injected into the submucosal layer of the rectum of athymic nude mice using trans-anal rectal cancer cell injection (TARCI). Thirty-six mice were inoculated with 10×10(5) cells and five mice were treated with sterile phosphate buffer solution. One to 4 weeks after cell injection, tumor growth was evaluated in vivo using T2-weighted MRI at 4.7T. A further group of animal (n=6) treated with ht-29_luc cells, with the same protocol, was monitored by optical imaging. In both groups, the presence of the primary tumor and of lymph nodes metastasis was confirmed by histology.SUBJECTS AND METHODSHt-29 cancer cells were directly injected into the submucosal layer of the rectum of athymic nude mice using trans-anal rectal cancer cell injection (TARCI). Thirty-six mice were inoculated with 10×10(5) cells and five mice were treated with sterile phosphate buffer solution. One to 4 weeks after cell injection, tumor growth was evaluated in vivo using T2-weighted MRI at 4.7T. A further group of animal (n=6) treated with ht-29_luc cells, with the same protocol, was monitored by optical imaging. In both groups, the presence of the primary tumor and of lymph nodes metastasis was confirmed by histology.In all animals, primary tumors were detectable by MRI, 1 week from TARCI. After 4 weeks primary tumors showed a mean longitudinal diameter of about 2cm. All animals developed regional lymph node metastases. Others organs (e.g. lung or liver) were not affected. In fat-suppressed, T2-weighted MRI, lymph nodes appeared as small areas characterized by hyper-intense signal compared to muscle. OI permitted evaluation of the primary tumor growth in perineal region.RESULTSIn all animals, primary tumors were detectable by MRI, 1 week from TARCI. After 4 weeks primary tumors showed a mean longitudinal diameter of about 2cm. All animals developed regional lymph node metastases. Others organs (e.g. lung or liver) were not affected. In fat-suppressed, T2-weighted MRI, lymph nodes appeared as small areas characterized by hyper-intense signal compared to muscle. OI permitted evaluation of the primary tumor growth in perineal region.TARCI of ht-29 cells into the rectum of nude mice is a feasible way to obtain a easily reproducible model of regional lymph node metastases could be monitored by magnetic resonance and optical imaging techniques.CONCLUSIONSTARCI of ht-29 cells into the rectum of nude mice is a feasible way to obtain a easily reproducible model of regional lymph node metastases could be monitored by magnetic resonance and optical imaging techniques. Abstract Object The work is aimed to develop a murine model of rectal cancer, which could be used to monitor lymph node metastasis development by magnetic resonance imaging (MRI) and optical imaging (OI) techniques. Subjects and methods Ht-29 cancer cells were directly injected into the submucosal layer of the rectum of athymic nude mice using trans-anal rectal cancer cell injection (TARCI). Thirty-six mice were inoculated with 10 × 105 cells and five mice were treated with sterile phosphate buffer solution. One to 4 weeks after cell injection, tumor growth was evaluated in vivo using T2-weighted MRI at 4.7T. A further group of animal ( n = 6) treated with ht-29_luc cells, with the same protocol, was monitored by optical imaging. In both groups, the presence of the primary tumor and of lymph nodes metastasis was confirmed by histology. Results In all animals, primary tumors were detectable by MRI, 1 week from TARCI. After 4 weeks primary tumors showed a mean longitudinal diameter of about 2 cm. All animals developed regional lymph node metastases. Others organs (e.g. lung or liver) were not affected. In fat-suppressed, T2-weighted MRI, lymph nodes appeared as small areas characterized by hyper-intense signal compared to muscle. OI permitted evaluation of the primary tumor growth in perineal region. Conclusions TARCI of ht-29 cells into the rectum of nude mice is a feasible way to obtain a easily reproducible model of regional lymph node metastases could be monitored by magnetic resonance and optical imaging techniques. |
| Author | Minicozzi, A.M. Cordiano, C. Calderan, L. Conti, G. Boschi, F. Sbarbati, A. Pacca, R. Marzola, P. Merigo, G. |
| Author_xml | – sequence: 1 givenname: A.M. surname: Minicozzi fullname: Minicozzi, A.M. organization: First division of general surgery, University of Verona, Verona, Italy – sequence: 2 givenname: G. surname: Conti fullname: Conti, G. email: giamaica@anatomy.univr.it organization: Section of human anatomy and histology, University of Verona, Verona, Italy – sequence: 3 givenname: G. surname: Merigo fullname: Merigo, G. organization: Section of human anatomy and histology, University of Verona, Verona, Italy – sequence: 4 givenname: P. surname: Marzola fullname: Marzola, P. organization: Section of human anatomy and histology, University of Verona, Verona, Italy – sequence: 5 givenname: F. surname: Boschi fullname: Boschi, F. organization: Section of human anatomy and histology, University of Verona, Verona, Italy – sequence: 6 givenname: L. surname: Calderan fullname: Calderan, L. organization: Section of human anatomy and histology, University of Verona, Verona, Italy – sequence: 7 givenname: R. surname: Pacca fullname: Pacca, R. organization: First division of general surgery, University of Verona, Verona, Italy – sequence: 8 givenname: A. surname: Sbarbati fullname: Sbarbati, A. organization: Section of human anatomy and histology, University of Verona, Verona, Italy – sequence: 9 givenname: C. surname: Cordiano fullname: Cordiano, C. organization: First division of general surgery, University of Verona, Verona, Italy |
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| CitedBy_id | crossref_primary_10_1016_j_tice_2022_101990 crossref_primary_10_1186_s13046_021_01851_8 crossref_primary_10_1016_j_jconrel_2015_09_041 crossref_primary_10_1007_s10585_024_10293_3 |
| Cites_doi | 10.1111/j.1463-1318.2006.01136.x 10.1016/j.jim.2006.01.022 10.1007/s00464-009-0650-9 10.2353/ajpath.2007.060773 10.1007/s00384-008-0569-y 10.1159/000085342 10.3748/wjg.v13.i32.4365 10.3233/CBM-2009-0544 10.1016/S0076-6879(04)86007-5 10.1016/j.jss.2008.05.028 10.1166/jbn.2009.030 10.1093/jnci/djk092 10.1002/path.1700350303 10.1002/jso.21217 10.1111/j.1463-1318.2004.00670.x 10.1111/j.1463-1318.2009.01769.x 10.1016/S0304-3835(00)00622-4 |
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| Keywords | Lymph node metastasis TARCI In vivo imaging |
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| References | Donigan, Norcross, Aversa, Colon, Smith, Maradero-Visbal (bib0020) 2009; 154 Jin, Liu, Ding, Yun, Liu, Zhou (bib0030) 2009; 24 Cèspedes, Espina, García-Cabezas, Trias, Boluda, Gómez del Pulgar (bib0025) 2007; 170 Koh, Brown, Collins (bib0005) 2009; 5 Van de Broek, Derore, Simoens (bib0055) 2006; 312 Tsutsumi, Kuwano, Morinaga, Shimura, Asao (bib0045) 2001; 169 Chang, Rodriguez-Bigas, Skibber, Moyer (bib0090) 2007; 99 Han Liang, Hong-Jie Zhan, Wang, Pan, Hao (bib0040) 2007; 13 Dukes (bib0065) 1932; 35 Donigan, Norcross, Aversa, Colon, Smith, Madero-Visbal (bib0035) 2009; 154 Keshava, Chaupis, Chan, Lin, Bokey, Dent (bib0070) 2007; 9 Bell, Sasaki, Sinclair, Chapuis, Bokey (bib0060) 2009; 11 Simunovic, Smith, Heald (bib0080) 2009; 99 Saravanakumar, Kim, Park, Rhee, Kwon (bib0085) 2009; 5 Heijstek, Kranenburg, Borel Rinkes (bib0015) 2005; 22 Donigan, Loh, Norcross, Li, Williamson, DeJesus (bib0050) 2010; 24 Marzola, Sbarbati (bib0010) 2004; 386 Thorn, Woodcock, Scott, Verbeke, Scott, Ambrose (bib0075) 2004; 6 Donigan (10.1016/j.biopha.2011.04.027_bib0020) 2009; 154 Van de Broek (10.1016/j.biopha.2011.04.027_bib0055) 2006; 312 Han Liang (10.1016/j.biopha.2011.04.027_bib0040) 2007; 13 Koh (10.1016/j.biopha.2011.04.027_bib0005) 2009; 5 Saravanakumar (10.1016/j.biopha.2011.04.027_bib0085) 2009; 5 Simunovic (10.1016/j.biopha.2011.04.027_bib0080) 2009; 99 Heijstek (10.1016/j.biopha.2011.04.027_bib0015) 2005; 22 Donigan (10.1016/j.biopha.2011.04.027_bib0035) 2009; 154 Tsutsumi (10.1016/j.biopha.2011.04.027_bib0045) 2001; 169 Bell (10.1016/j.biopha.2011.04.027_bib0060) 2009; 11 Chang (10.1016/j.biopha.2011.04.027_bib0090) 2007; 99 Cèspedes (10.1016/j.biopha.2011.04.027_bib0025) 2007; 170 Keshava (10.1016/j.biopha.2011.04.027_bib0070) 2007; 9 Jin (10.1016/j.biopha.2011.04.027_bib0030) 2009; 24 Dukes (10.1016/j.biopha.2011.04.027_bib0065) 1932; 35 Marzola (10.1016/j.biopha.2011.04.027_bib0010) 2004; 386 Donigan (10.1016/j.biopha.2011.04.027_bib0050) 2010; 24 Thorn (10.1016/j.biopha.2011.04.027_bib0075) 2004; 6 |
| References_xml | – volume: 22 start-page: 16 year: 2005 end-page: 25 ident: bib0015 article-title: Mouse model of colorectal cancer and liver metastases publication-title: Dig Surg – volume: 154 start-page: 299 year: 2009 end-page: 303 ident: bib0020 article-title: Novel murine model for colon cancer: non-operative trans-anal rectal injection publication-title: J Surg Res – volume: 13 start-page: 4365 year: 2007 end-page: 4371 ident: bib0040 article-title: Change in expression of apoptosis genes after hyperthermia, chemotherapy and radiotherapy in human colon cancer transplanted into nude mice publication-title: World J Gastroenterol – volume: 24 start-page: 642 year: 2010 end-page: 647 ident: bib0050 article-title: A metastatic colon cancer model using non-operative trans-anal rectal injection publication-title: Surg Endosc – volume: 170 start-page: 1077 year: 2007 end-page: 1085 ident: bib0025 article-title: Orthotopic microinjection of human colon cancer cells in nude mice induces tumor foci in all clinically relevant metastatic sites publication-title: Am J Pathol – volume: 11 start-page: 443 year: 2009 end-page: 449 ident: bib0060 article-title: Understanding the anatomy of lymphatic drainage and the use of blue-dye mapping to determine the extent of lymphoadenoctomy in rectal cancer surgery: unresolved issues publication-title: Colorectal Dis – volume: 169 start-page: 77 year: 2001 end-page: 85 ident: bib0045 article-title: Animal model of para-aortic lymph node metastasis publication-title: Cancer Lett – volume: 9 start-page: 609 year: 2007 end-page: 618 ident: bib0070 article-title: The significance on involvement of a free serosal surface for recurrence and survival following resection of clinicopathological Stage B and C rectal cancer publication-title: Colorectal Dis – volume: 24 start-page: 41 year: 2009 end-page: 47 ident: bib0030 article-title: A simple colostomy implantation model for evaluating colon cancer publication-title: Int J Colorectal Dis – volume: 5 start-page: 20 year: 2009 end-page: 35 ident: bib0085 article-title: Current status of nanoparticle-based imaging agents for early diagnosis of cancer and atherosclerosis publication-title: J Biomed Nanotechnol – volume: 99 start-page: 256 year: 2009 end-page: 259 ident: bib0080 article-title: Rectal cancer surgery and regional lymph nodes publication-title: J Surg Oncol – volume: 35 start-page: 323 year: 1932 end-page: 332 ident: bib0065 article-title: The classification of cancer of the rectum publication-title: J Pathol Bacteriol – volume: 99 start-page: 433 year: 2007 end-page: 441 ident: bib0090 article-title: Lymph node evaluation and survival after curative resection of colon cancer: systematic review publication-title: Natl Cancer Inst – volume: 6 start-page: 356 year: 2004 end-page: 361 ident: bib0075 article-title: What factors affect lymph node yield in surgery for rectal cancer? publication-title: Colorectal Dis – volume: 386 start-page: 177 year: 2004 end-page: 200 ident: bib0010 article-title: Magnetic resonance imaging in animal models of pathologies publication-title: Methods Enzymol – volume: 312 start-page: 12 year: 2006 end-page: 19 ident: bib0055 article-title: Anatomy and nomenclature of murine lymph nodes: descriptive study and nomenclatory standardization in BALB/cAnNCrl mice publication-title: J Immunol Methods – volume: 5 start-page: 89 year: 2009 end-page: 98 ident: bib0005 article-title: Nanoparticles in rectal cancer imaging publication-title: Cancer Biomark – volume: 154 start-page: 299 year: 2009 end-page: 303 ident: bib0035 article-title: Novel murine model for colon cancer: non-operative trans-anal rectal injection publication-title: J Surg Res – volume: 9 start-page: 609 year: 2007 ident: 10.1016/j.biopha.2011.04.027_bib0070 article-title: The significance on involvement of a free serosal surface for recurrence and survival following resection of clinicopathological Stage B and C rectal cancer publication-title: Colorectal Dis doi: 10.1111/j.1463-1318.2006.01136.x – volume: 312 start-page: 12 year: 2006 ident: 10.1016/j.biopha.2011.04.027_bib0055 article-title: Anatomy and nomenclature of murine lymph nodes: descriptive study and nomenclatory standardization in BALB/cAnNCrl mice publication-title: J Immunol Methods doi: 10.1016/j.jim.2006.01.022 – volume: 24 start-page: 642 year: 2010 ident: 10.1016/j.biopha.2011.04.027_bib0050 article-title: A metastatic colon cancer model using non-operative trans-anal rectal injection publication-title: Surg Endosc doi: 10.1007/s00464-009-0650-9 – volume: 170 start-page: 1077 year: 2007 ident: 10.1016/j.biopha.2011.04.027_bib0025 article-title: Orthotopic microinjection of human colon cancer cells in nude mice induces tumor foci in all clinically relevant metastatic sites publication-title: Am J Pathol doi: 10.2353/ajpath.2007.060773 – volume: 24 start-page: 41 year: 2009 ident: 10.1016/j.biopha.2011.04.027_bib0030 article-title: A simple colostomy implantation model for evaluating colon cancer publication-title: Int J Colorectal Dis doi: 10.1007/s00384-008-0569-y – volume: 22 start-page: 16 year: 2005 ident: 10.1016/j.biopha.2011.04.027_bib0015 article-title: Mouse model of colorectal cancer and liver metastases publication-title: Dig Surg doi: 10.1159/000085342 – volume: 13 start-page: 4365 year: 2007 ident: 10.1016/j.biopha.2011.04.027_bib0040 article-title: Change in expression of apoptosis genes after hyperthermia, chemotherapy and radiotherapy in human colon cancer transplanted into nude mice publication-title: World J Gastroenterol doi: 10.3748/wjg.v13.i32.4365 – volume: 5 start-page: 89 year: 2009 ident: 10.1016/j.biopha.2011.04.027_bib0005 article-title: Nanoparticles in rectal cancer imaging publication-title: Cancer Biomark doi: 10.3233/CBM-2009-0544 – volume: 386 start-page: 177 year: 2004 ident: 10.1016/j.biopha.2011.04.027_bib0010 article-title: Magnetic resonance imaging in animal models of pathologies publication-title: Methods Enzymol doi: 10.1016/S0076-6879(04)86007-5 – volume: 154 start-page: 299 issue: 2 year: 2009 ident: 10.1016/j.biopha.2011.04.027_bib0035 article-title: Novel murine model for colon cancer: non-operative trans-anal rectal injection publication-title: J Surg Res doi: 10.1016/j.jss.2008.05.028 – volume: 5 start-page: 20 year: 2009 ident: 10.1016/j.biopha.2011.04.027_bib0085 article-title: Current status of nanoparticle-based imaging agents for early diagnosis of cancer and atherosclerosis publication-title: J Biomed Nanotechnol doi: 10.1166/jbn.2009.030 – volume: 99 start-page: 433 year: 2007 ident: 10.1016/j.biopha.2011.04.027_bib0090 article-title: Lymph node evaluation and survival after curative resection of colon cancer: systematic review publication-title: Natl Cancer Inst doi: 10.1093/jnci/djk092 – volume: 35 start-page: 323 year: 1932 ident: 10.1016/j.biopha.2011.04.027_bib0065 article-title: The classification of cancer of the rectum publication-title: J Pathol Bacteriol doi: 10.1002/path.1700350303 – volume: 99 start-page: 256 year: 2009 ident: 10.1016/j.biopha.2011.04.027_bib0080 article-title: Rectal cancer surgery and regional lymph nodes publication-title: J Surg Oncol doi: 10.1002/jso.21217 – volume: 154 start-page: 299 year: 2009 ident: 10.1016/j.biopha.2011.04.027_bib0020 article-title: Novel murine model for colon cancer: non-operative trans-anal rectal injection publication-title: J Surg Res doi: 10.1016/j.jss.2008.05.028 – volume: 6 start-page: 356 year: 2004 ident: 10.1016/j.biopha.2011.04.027_bib0075 article-title: What factors affect lymph node yield in surgery for rectal cancer? publication-title: Colorectal Dis doi: 10.1111/j.1463-1318.2004.00670.x – volume: 11 start-page: 443 year: 2009 ident: 10.1016/j.biopha.2011.04.027_bib0060 article-title: Understanding the anatomy of lymphatic drainage and the use of blue-dye mapping to determine the extent of lymphoadenoctomy in rectal cancer surgery: unresolved issues publication-title: Colorectal Dis doi: 10.1111/j.1463-1318.2009.01769.x – volume: 169 start-page: 77 year: 2001 ident: 10.1016/j.biopha.2011.04.027_bib0045 article-title: Animal model of para-aortic lymph node metastasis publication-title: Cancer Lett doi: 10.1016/S0304-3835(00)00622-4 |
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| SubjectTerms | Animals Disease Models, Animal Early Detection of Cancer - methods HT29 Cells Humans In vivo imaging Internal Medicine Luciferases - biosynthesis Luciferases - genetics Luminescent Agents Lymph node metastasis Lymph Nodes - metabolism Lymph Nodes - pathology Lymphatic Metastasis - diagnosis Lymphatic Metastasis - pathology Magnetic Resonance Imaging Male Medical Education Mice Mice, Nude Molecular Imaging Neoplasm Proteins - biosynthesis Neoplasm Proteins - genetics Neoplasm Transplantation - methods Pilot Projects Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Rectal Neoplasms - diagnosis Rectal Neoplasms - genetics Rectal Neoplasms - metabolism Rectal Neoplasms - pathology Rectum - metabolism Rectum - pathology Reproducibility of Results TARCI Tumor Burden |
| Title | A new model of rectal cancer with regional lymph node metastasis allowing in vivo evaluation by imaging biomarkers |
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