Identification of Six Small Heat Shock Protein Genes in Ostrinia furnacalis (Lepidoptera: Pyralidae) and Analysis of Their Expression Patterns in Response to Environmental Stressors

• Published in: Journal of insect science (Tucson, Ariz.) Vol. 22; no. 6
• Main Authors: Ruan, Hong-Yun, Meng, Jian-Yu, Yang, Chang-Li, Zhou, Lv, Zhang, Chang-Yu
• Format: Journal Article
• Language: English
• Published: US Oxford University Press 01.11.2022
• Subjects: Adults; Biological research; Biology, Experimental; Crop production; Crystallin; Developmental stages; Environmental stress; Females; Genes; Genetic aspects; Heat shock proteins; Identification and classification; Insects; Males; Moths; Ostrinia furnacalis; Physiological aspects; Polymerase chain reaction; Proteins; Small heat shock proteins; Stresses; China; small heat shock protein; UV-A; extreme temperature
• ISSN: 1536-2442; 1536-2442
• DOI: 10.1093/jisesa/ieac069

Abstract Ostrinia furnacalis (Guenée) is a major insect pest in maize production that is highly adaptable to the environment. Small heat shock proteins (sHsps) are a class of chaperone proteins that play an important role in insect responses to various environmental stresses. The present study aimed to clarify the responses of six O. furnacalis sHsps to environmental stressors. In particular, we cloned six sHsp genes, namely, OfHsp24.2, OfHsp21.3, OfHsp20.7, OfHsp21.8, OfHsp29.7, and OfHsp19.9, from O. furnacalis. The putative proteins encoded by these genes contained a typical α-crystallin domain. Real-time quantitative polymerase chain reaction was used to analyze the differences in the expression of these genes at different developmental stages, in different tissues of male and female adults, and in O. furnacalis under UV-A and extreme temperature stresses. The six OfsHsp genes were expressed at significantly different levels based on the developmental stage and tissue type in male and female adults. Furthermore, all OfsHsp genes were significantly upregulated in both male and female adults under extreme temperature and UV-A stresses. Thus, O. furnacalis OfsHsp genes play important and unique regulatory roles in the developmental stages of the insect and in response to various environmental stressors.