Identification of a Melanoma Marker Derived from Melanoma-Associated Endogenous Retroviruses
We previously described the expression of melanoma-associated endogenous retrovirus (MERV) proteins and viral particles in human melanomas and metastases. The objective of the present study was to determine whether a humoral immune response to MERV proteins occurs in melanoma. Candidate B-cell epito...
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| Published in | Cancer research (Chicago, Ill.) Vol. 66; no. 3; pp. 1658 - 1663 |
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| Main Authors | , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Philadelphia, PA
American Association for Cancer Research
01.02.2006
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0008-5472 1538-7445 |
| DOI | 10.1158/0008-5472.CAN-05-2452 |
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| Abstract | We previously described the expression of melanoma-associated endogenous retrovirus (MERV) proteins and viral particles in human melanomas and metastases. The objective of the present study was to determine whether a humoral immune response to MERV proteins occurs in melanoma. Candidate B-cell epitopes on MERV proteins were predicted using bioinformatic screening. The reactivity of MERV peptides corresponding to the predicted epitopes with antibodies prevalent in sera of melanoma patients was analyzed. An immunodominant peptide located in the env protein of MERV was identified. Subsequent analyzes using 81 samples from stage I to stage IV melanoma patients and 95 sera from healthy subjects revealed statistically significant differences in seroprevalence of antibodies in melanoma sera samples when compared with reference samples from healthy subjects. The prevalence of anti-MERV antibodies in melanoma patient sera was confirmed by immunofluorescence on env-transfected cells. These data indicate the potential of this candidate peptide as target for diagnosis and immunotherapy. (Cancer Res 2006; 66(3): 1658-63) |
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| AbstractList | We previously described the expression of melanoma-associated endogenous retrovirus (MERV) proteins and viral particles in human melanomas and metastases. The objective of the present study was to determine whether a humoral immune response to MERV proteins occurs in melanoma. Candidate B-cell epitopes on MERV proteins were predicted using bioinformatic screening. The reactivity of MERV peptides corresponding to the predicted epitopes with antibodies prevalent in sera of melanoma patients was analyzed. An immunodominant peptide located in the env protein of MERV was identified. Subsequent analyzes using 81 samples from stage I to stage IV melanoma patients and 95 sera from healthy subjects revealed statistically significant differences in seroprevalence of antibodies in melanoma sera samples when compared with reference samples from healthy subjects. The prevalence of anti-MERV antibodies in melanoma patient sera was confirmed by immunofluorescence on env-transfected cells. These data indicate the potential of this candidate peptide as target for diagnosis and immunotherapy.We previously described the expression of melanoma-associated endogenous retrovirus (MERV) proteins and viral particles in human melanomas and metastases. The objective of the present study was to determine whether a humoral immune response to MERV proteins occurs in melanoma. Candidate B-cell epitopes on MERV proteins were predicted using bioinformatic screening. The reactivity of MERV peptides corresponding to the predicted epitopes with antibodies prevalent in sera of melanoma patients was analyzed. An immunodominant peptide located in the env protein of MERV was identified. Subsequent analyzes using 81 samples from stage I to stage IV melanoma patients and 95 sera from healthy subjects revealed statistically significant differences in seroprevalence of antibodies in melanoma sera samples when compared with reference samples from healthy subjects. The prevalence of anti-MERV antibodies in melanoma patient sera was confirmed by immunofluorescence on env-transfected cells. These data indicate the potential of this candidate peptide as target for diagnosis and immunotherapy. We previously described the expression of melanoma-associated endogenous retrovirus (MERV) proteins and viral particles in human melanomas and metastases. The objective of the present study was to determine whether a humoral immune response to MERV proteins occurs in melanoma. Candidate B-cell epitopes on MERV proteins were predicted using bioinformatic screening. The reactivity of MERV peptides corresponding to the predicted epitopes with antibodies prevalent in sera of melanoma patients was analyzed. An immunodominant peptide located in the env protein of MERV was identified. Subsequent analyzes using 81 samples from stage I to stage IV melanoma patients and 95 sera from healthy subjects revealed statistically significant differences in seroprevalence of antibodies in melanoma sera samples when compared with reference samples from healthy subjects. The prevalence of anti-MERV antibodies in melanoma patient sera was confirmed by immunofluorescence on env-transfected cells. These data indicate the potential of this candidate peptide as target for diagnosis and immunotherapy. (Cancer Res 2006; 66(3): 1658-63) We previously described the expression of melanoma-associated endogenous retrovirus (MERV) proteins and viral particles in human melanomas and metastases. The objective of the present study was to determine whether a humoral immune response to MERV proteins occurs in melanoma. Candidate B-cell epitopes on MERV proteins were predicted using bioinformatic screening. The reactivity of MERV peptides corresponding to the predicted epitopes with antibodies prevalent in sera of melanoma patients was analyzed. An immunodominant peptide located in the env protein of MERV was identified. Subsequent analyzes using 81 samples from stage I to stage IV melanoma patients and 95 sera from healthy subjects revealed statistically significant differences in seroprevalence of antibodies in melanoma sera samples when compared with reference samples from healthy subjects. The prevalence of anti-MERV antibodies in melanoma patient sera was confirmed by immunofluorescence on env-transfected cells. These data indicate the potential of this candidate peptide as target for diagnosis and immunotherapy. |
| Author | Humer, Johannes Valencak, Julia Bergmann, Michael Pehamberger, Hubert Kurz, Martin Mayer, Bernd Grassauer, Andreas Rapberger, Ronald Muster, Thomas Löwer, Roswitha Wolff, Klaus Hahn, Silvia Waltenberger, Andrea |
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| Cites_doi | 10.1586/14737159.3.2.163 10.1093/jnci/60.6.1305 10.1016/S0092-8674(00)80338-4 10.1038/sj.onc.1208562 10.1046/j.1365-2249.2002.01735.x 10.1038/6766 10.1200/JCO.2001.19.16.3635 10.1128/jvi.71.6.4581-4588.1997 10.1038/sj.leu.2402355 10.1099/0022-1317-65-5-887 10.1073/pnas.72.9.3697 10.1006/viro.1993.1487 10.1158/0008-5472.CAN-04-2983 10.1016/0014-2964(74)90024-3 10.1158/0008-5472.CAN-04-4231 10.1586/14737159.3.3.303 10.1073/pnas.93.11.5177 10.1002/ijc.11649 |
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| Keywords | Antineoplastic agent Virus Endogenous Immune response Antibody Immunotherapy Melanoma associated endogenous retrovirus Malignant melanoma Biological marker Malignant tumor Diagnosis Humoral immunity |
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| SubjectTerms | Antibodies, Neoplasm - blood Antibodies, Neoplasm - immunology Antineoplastic agents Biological and medical sciences Biomarkers, Tumor - immunology Cross Reactions Dermatology Endogenous Retroviruses - immunology Epitopes, B-Lymphocyte - immunology HeLa Cells Humans Immunodominant Epitopes - immunology Immunotherapy Medical sciences Melanoma - blood Melanoma - immunology Melanoma - pathology Melanoma - virology Neoplasm Staging Pharmacology. Drug treatments Tumors of the skin and soft tissue. Premalignant lesions Viral Envelope Proteins - immunology |
| Title | Identification of a Melanoma Marker Derived from Melanoma-Associated Endogenous Retroviruses |
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