MARK-AGE standard operating procedures (SOPs): A successful effort

•Standard operating procedures for sample collection and preparation within MARK-AGE.•Samples processing following a master protocol elaborated for MARK-AGE purposes.•Temporary storage of MARK-AGE samples at a central Biobank.•Logistic challenges of MARK-AGE project. Within the MARK-AGE project, a p...

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Published inMechanisms of ageing and development Vol. 151; pp. 18 - 25
Main Authors Moreno-Villanueva, María, Capri, Miriam, Breusing, Nicolle, Siepelmeyer, Anne, Sevini, Federica, Ghezzo, Alessandro, Craen, Anton J.M.de, Hervonen, Antti, Hurme, Mikko, Schön, Christiane, Grune, Tilman, Franceschi, Claudio, Bürkle, Alexander
Format Journal Article
LanguageEnglish
Published Ireland Elsevier Ireland Ltd 01.11.2015
Subjects
Online AccessGet full text
ISSN0047-6374
1872-6216
1872-6216
DOI10.1016/j.mad.2015.03.007

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Abstract •Standard operating procedures for sample collection and preparation within MARK-AGE.•Samples processing following a master protocol elaborated for MARK-AGE purposes.•Temporary storage of MARK-AGE samples at a central Biobank.•Logistic challenges of MARK-AGE project. Within the MARK-AGE project, a population study (3337 subjects) was conducted to identify a set of biomarkers of ageing which, as a combination of parameters with appropriate weighting, would measure biological age better than any single marker. The MARK-AGE project involves 14 European countries and a total of 26 research centres. In such a study, standard operating procedures (SOPs) are an essential task, which are binding for all MARK-AGE Beneficiaries. The SOPs cover all aspects of subject’s recruitment, collection, shipment and distribution of biological samples (blood and its components, buccal mucosa cells or BMC and urine) as well as the anthropometric measurements and questionnaires.
AbstractList Within the MARK-AGE project, a population study (3337 subjects) was conducted to identify a set of biomarkers of ageing which, as a combination of parameters with appropriate weighting, would measure biological age better than any single marker. The MARK-AGE project involves 14 European countries and a total of 26 research centres. In such a study, standard operating procedures (SOPs) are an essential task, which are binding for all MARK-AGE Beneficiaries. The SOPs cover all aspects of subject's recruitment, collection, shipment and distribution of biological samples (blood and its components, buccal mucosa cells or BMC and urine) as well as the anthropometric measurements and questionnaires.
•Standard operating procedures for sample collection and preparation within MARK-AGE.•Samples processing following a master protocol elaborated for MARK-AGE purposes.•Temporary storage of MARK-AGE samples at a central Biobank.•Logistic challenges of MARK-AGE project. Within the MARK-AGE project, a population study (3337 subjects) was conducted to identify a set of biomarkers of ageing which, as a combination of parameters with appropriate weighting, would measure biological age better than any single marker. The MARK-AGE project involves 14 European countries and a total of 26 research centres. In such a study, standard operating procedures (SOPs) are an essential task, which are binding for all MARK-AGE Beneficiaries. The SOPs cover all aspects of subject’s recruitment, collection, shipment and distribution of biological samples (blood and its components, buccal mucosa cells or BMC and urine) as well as the anthropometric measurements and questionnaires.
Within the MARK-AGE project, a population study (3337 subjects) was conducted to identify a set of biomarkers of ageing which, as a combination of parameters with appropriate weighting, would measure biological age better than any single marker. The MARK-AGE project involves 14 European countries and a total of 26 research centres. In such a study, standard operating procedures (SOPs) are an essential task, which are binding for all MARK-AGE Beneficiaries. The SOPs cover all aspects of subject's recruitment, collection, shipment and distribution of biological samples (blood and its components, buccal mucosa cells or BMC and urine) as well as the anthropometric measurements and questionnaires.Within the MARK-AGE project, a population study (3337 subjects) was conducted to identify a set of biomarkers of ageing which, as a combination of parameters with appropriate weighting, would measure biological age better than any single marker. The MARK-AGE project involves 14 European countries and a total of 26 research centres. In such a study, standard operating procedures (SOPs) are an essential task, which are binding for all MARK-AGE Beneficiaries. The SOPs cover all aspects of subject's recruitment, collection, shipment and distribution of biological samples (blood and its components, buccal mucosa cells or BMC and urine) as well as the anthropometric measurements and questionnaires.
Author Hervonen, Antti
Breusing, Nicolle
Franceschi, Claudio
Craen, Anton J.M.de
Hurme, Mikko
Schön, Christiane
Moreno-Villanueva, María
Grune, Tilman
Bürkle, Alexander
Siepelmeyer, Anne
Capri, Miriam
Sevini, Federica
Ghezzo, Alessandro
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  surname: Ghezzo
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  organization: National Association “Famiglie di Persone con Disabilita Affettiva e/o Relazionale” (ANFFAS) Onlus, Macerata, Italy
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  givenname: Anton J.M.de
  surname: Craen
  fullname: Craen, Anton J.M.de
  organization: Department of Gerontology and Geriatrics, Leiden University Medical Center, Leiden, The Netherlands
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  givenname: Antti
  surname: Hervonen
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  organization: School of Medicine, University of Tampere, 33014 Tampere, Finland
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  organization: BioTeSys GmbH, 73728 Esslingen, Germany
– sequence: 11
  givenname: Tilman
  surname: Grune
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  organization: Institute of Nutritional Medicine, University of Hohenheim, 70599 Stuttgart, Germany
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  givenname: Claudio
  surname: Franceschi
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  givenname: Alexander
  surname: Bürkle
  fullname: Bürkle, Alexander
  organization: Molecular Toxicology Group, Department of Biology, University of Konstanz, 78457 Konstanz, Germany
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Keywords Biobank
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Standard operating procedures
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Aging - urine
Biobank
Biomarkers - blood
Biomarkers - urine
Female
Human studies
Humans
Male
Standard operating procedures
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