Platelet Activation in Ovarian Cancer Ascites: Assessment of GPIIb/IIIa and PF4 in Small Extracellular Vesicles by Nano-Flow Cytometry Analysis
In ovarian cancer, ascites represent the microenvironment in which the platelets extravasate to play their role in the disease progression. We aimed to develop an assay to measure ascites’ platelet activation. We enriched small extracellular vesicles (EVs) (40–200 nm) from ascites of high-grade epit...
Saved in:
| Published in | Cancers Vol. 14; no. 17; p. 4100 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Basel
MDPI AG
24.08.2022
MDPI |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2072-6694 2072-6694 |
| DOI | 10.3390/cancers14174100 |
Cover
| Abstract | In ovarian cancer, ascites represent the microenvironment in which the platelets extravasate to play their role in the disease progression. We aimed to develop an assay to measure ascites’ platelet activation. We enriched small extracellular vesicles (EVs) (40–200 nm) from ascites of high-grade epithelial ovarian cancer patients (n = 12) using precipitation with polyethylene glycol, and we conducted single-particle phenotyping analysis by nano-flow cytometry after labelling and ultra-centrifugation. Atomic force microscopy single-particle nanomechanical analysis showed heterogeneous distributions in the size of the precipitated particles and their mechanical stiffness. Samples were fluorescently labelled with antibodies specific to the platelet markers GPIIb/IIIa and PF4, showing 2.6 to 18.16% of all particles stained positive for the biomarkers and, simultaneously, the EV membrane labelling. Single-particle phenotyping analysis allowed us to quantify the total number of non-EV particles, the number of small-EVs and the number of platelet-derived small-EVs, providing a platelet activation assessment independent of the ascites volume. The percentage of platelet-derived small-EVs was positively correlated with platelet distribution width to platelet count in sera (PDW/PLT). Overall, we presented a high-throughput method that can be helpful in future studies to determine the correlation between the extent of platelet activation in ascites and disease status. |
|---|---|
| AbstractList | In ovarian cancer, ascites represent the microenvironment in which the platelets extravasate to play their role in the disease progression. We aimed to develop an assay to measure ascites’ platelet activation. We enriched small extracellular vesicles (EVs) (40–200 nm) from ascites of high-grade epithelial ovarian cancer patients (n = 12) using precipitation with polyethylene glycol, and we conducted single-particle phenotyping analysis by nano-flow cytometry after labelling and ultra-centrifugation. Atomic force microscopy single-particle nanomechanical analysis showed heterogeneous distributions in the size of the precipitated particles and their mechanical stiffness. Samples were fluorescently labelled with antibodies specific to the platelet markers GPIIb/IIIa and PF4, showing 2.6 to 18.16% of all particles stained positive for the biomarkers and, simultaneously, the EV membrane labelling. Single-particle phenotyping analysis allowed us to quantify the total number of non-EV particles, the number of small-EVs and the number of platelet-derived small-EVs, providing a platelet activation assessment independent of the ascites volume. The percentage of platelet-derived small-EVs was positively correlated with platelet distribution width to platelet count in sera (PDW/PLT). Overall, we presented a high-throughput method that can be helpful in future studies to determine the correlation between the extent of platelet activation in ascites and disease status. Platelets play a critical role in coagulation and fibrinolysis processes, but recent literature also indicates their central involvement in immune response, cancer progression and metastasis. During platelet activation, small extracellular vesicles (EVs) are released. The ascites are a fluid developing in the peritoneum of ovarian cancer patients in an advanced stage. This study analysed the expression of platelet markers GPIIb/IIIa and PF4 in small-EVs populations isolated from the ascitic fluid of patients with advanced ovarian cancer. The percentage of platelet-derived small-EVs was positively correlated with platelet distribution width to platelet count in sera (PDW/PLT), a surrogate indicator of platelet activation. Overall, we presented a method that can be helpful in future studies to determine the correlation between the extent of platelet activation in ascites and disease status. In ovarian cancer, ascites represent the microenvironment in which the platelets extravasate to play their role in the disease progression. We aimed to develop an assay to measure ascites’ platelet activation. We enriched small extracellular vesicles (EVs) (40–200 nm) from ascites of high-grade epithelial ovarian cancer patients (n = 12) using precipitation with polyethylene glycol, and we conducted single-particle phenotyping analysis by nano-flow cytometry after labelling and ultra-centrifugation. Atomic force microscopy single-particle nanomechanical analysis showed heterogeneous distributions in the size of the precipitated particles and their mechanical stiffness. Samples were fluorescently labelled with antibodies specific to the platelet markers GPIIb/IIIa and PF4, showing 2.6 to 18.16% of all particles stained positive for the biomarkers and, simultaneously, the EV membrane labelling. Single-particle phenotyping analysis allowed us to quantify the total number of non-EV particles, the number of small-EVs and the number of platelet-derived small-EVs, providing a platelet activation assessment independent of the ascites volume. The percentage of platelet-derived small-EVs was positively correlated with platelet distribution width to platelet count in sera (PDW/PLT). Overall, we presented a high-throughput method that can be helpful in future studies to determine the correlation between the extent of platelet activation in ascites and disease status. Simple SummaryPlatelets play a critical role in coagulation and fibrinolysis processes, but recent literature also indicates their central involvement in immune response, cancer progression and metastasis. During platelet activation, small extracellular vesicles (EVs) are released. The ascites are a fluid developing in the peritoneum of ovarian cancer patients in an advanced stage. This study analysed the expression of platelet markers GPIIb/IIIa and PF4 in small-EVs populations isolated from the ascitic fluid of patients with advanced ovarian cancer. The percentage of platelet-derived small-EVs was positively correlated with platelet distribution width to platelet count in sera (PDW/PLT), a surrogate indicator of platelet activation. Overall, we presented a method that can be helpful in future studies to determine the correlation between the extent of platelet activation in ascites and disease status.AbstractIn ovarian cancer, ascites represent the microenvironment in which the platelets extravasate to play their role in the disease progression. We aimed to develop an assay to measure ascites’ platelet activation. We enriched small extracellular vesicles (EVs) (40–200 nm) from ascites of high-grade epithelial ovarian cancer patients (n = 12) using precipitation with polyethylene glycol, and we conducted single-particle phenotyping analysis by nano-flow cytometry after labelling and ultra-centrifugation. Atomic force microscopy single-particle nanomechanical analysis showed heterogeneous distributions in the size of the precipitated particles and their mechanical stiffness. Samples were fluorescently labelled with antibodies specific to the platelet markers GPIIb/IIIa and PF4, showing 2.6 to 18.16% of all particles stained positive for the biomarkers and, simultaneously, the EV membrane labelling. Single-particle phenotyping analysis allowed us to quantify the total number of non-EV particles, the number of small-EVs and the number of platelet-derived small-EVs, providing a platelet activation assessment independent of the ascites volume. The percentage of platelet-derived small-EVs was positively correlated with platelet distribution width to platelet count in sera (PDW/PLT). Overall, we presented a high-throughput method that can be helpful in future studies to determine the correlation between the extent of platelet activation in ascites and disease status. Platelets play a critical role in coagulation and fibrinolysis processes, but recent literature also indicates their central involvement in immune response, cancer progression and metastasis. During platelet activation, small extracellular vesicles (EVs) are released. The ascites are a fluid developing in the peritoneum of ovarian cancer patients in an advanced stage. This study analysed the expression of platelet markers GPIIb/IIIa and PF4 in small-EVs populations isolated from the ascitic fluid of patients with advanced ovarian cancer. The percentage of platelet-derived small-EVs was positively correlated with platelet distribution width to platelet count in sera (PDW/PLT), a surrogate indicator of platelet activation. Overall, we presented a method that can be helpful in future studies to determine the correlation between the extent of platelet activation in ascites and disease status. In ovarian cancer, ascites represent the microenvironment in which the platelets extravasate to play their role in the disease progression. We aimed to develop an assay to measure ascites' platelet activation. We enriched small extracellular vesicles (EVs) (40-200 nm) from ascites of high-grade epithelial ovarian cancer patients (n = 12) using precipitation with polyethylene glycol, and we conducted single-particle phenotyping analysis by nano-flow cytometry after labelling and ultra-centrifugation. Atomic force microscopy single-particle nanomechanical analysis showed heterogeneous distributions in the size of the precipitated particles and their mechanical stiffness. Samples were fluorescently labelled with antibodies specific to the platelet markers GPIIb/IIIa and PF4, showing 2.6 to 18.16% of all particles stained positive for the biomarkers and, simultaneously, the EV membrane labelling. Single-particle phenotyping analysis allowed us to quantify the total number of non-EV particles, the number of small-EVs and the number of platelet-derived small-EVs, providing a platelet activation assessment independent of the ascites volume. The percentage of platelet-derived small-EVs was positively correlated with platelet distribution width to platelet count in sera (PDW/PLT). Overall, we presented a high-throughput method that can be helpful in future studies to determine the correlation between the extent of platelet activation in ascites and disease status.In ovarian cancer, ascites represent the microenvironment in which the platelets extravasate to play their role in the disease progression. We aimed to develop an assay to measure ascites' platelet activation. We enriched small extracellular vesicles (EVs) (40-200 nm) from ascites of high-grade epithelial ovarian cancer patients (n = 12) using precipitation with polyethylene glycol, and we conducted single-particle phenotyping analysis by nano-flow cytometry after labelling and ultra-centrifugation. Atomic force microscopy single-particle nanomechanical analysis showed heterogeneous distributions in the size of the precipitated particles and their mechanical stiffness. Samples were fluorescently labelled with antibodies specific to the platelet markers GPIIb/IIIa and PF4, showing 2.6 to 18.16% of all particles stained positive for the biomarkers and, simultaneously, the EV membrane labelling. Single-particle phenotyping analysis allowed us to quantify the total number of non-EV particles, the number of small-EVs and the number of platelet-derived small-EVs, providing a platelet activation assessment independent of the ascites volume. The percentage of platelet-derived small-EVs was positively correlated with platelet distribution width to platelet count in sera (PDW/PLT). Overall, we presented a high-throughput method that can be helpful in future studies to determine the correlation between the extent of platelet activation in ascites and disease status. |
| Audience | Academic |
| Author | Brucale, Marco Peacock, Ben Ricci, Giuseppe Romano, Federico Biffi, Stefania Tassinari, Sara Valle, Francesco Lees, Rebecca Bortot, Barbara Mangogna, Alessandro |
| AuthorAffiliation | 3 Consorzio per lo Sviluppo dei Sistemi a Grande Interfase (CSGI), 50019 Sesto Fiorentino, Italy 2 NanoFCM Co., Ltd., MediCity, Nottingham NG90 6BH, UK 5 Department of Medical, Surgical and Health Science, University of Trieste, 34127 Trieste, Italy 4 Consiglio Nazionale delle Ricerche, Istituto per lo Studio dei Materiali Nanostrutturati (CNRISMN), 40129 Bologna, Italy 1 Obstetrics and Gynecology, Institute for Maternal and Child Health, IRCCS Burlo Garofolo, 34137 Trieste, Italy |
| AuthorAffiliation_xml | – name: 3 Consorzio per lo Sviluppo dei Sistemi a Grande Interfase (CSGI), 50019 Sesto Fiorentino, Italy – name: 4 Consiglio Nazionale delle Ricerche, Istituto per lo Studio dei Materiali Nanostrutturati (CNRISMN), 40129 Bologna, Italy – name: 2 NanoFCM Co., Ltd., MediCity, Nottingham NG90 6BH, UK – name: 1 Obstetrics and Gynecology, Institute for Maternal and Child Health, IRCCS Burlo Garofolo, 34137 Trieste, Italy – name: 5 Department of Medical, Surgical and Health Science, University of Trieste, 34127 Trieste, Italy |
| Author_xml | – sequence: 1 givenname: Barbara surname: Bortot fullname: Bortot, Barbara – sequence: 2 givenname: Alessandro orcidid: 0000-0001-7910-1274 surname: Mangogna fullname: Mangogna, Alessandro – sequence: 3 givenname: Ben orcidid: 0000-0002-7823-8719 surname: Peacock fullname: Peacock, Ben – sequence: 4 givenname: Rebecca surname: Lees fullname: Lees, Rebecca – sequence: 5 givenname: Francesco orcidid: 0000-0001-5793-7206 surname: Valle fullname: Valle, Francesco – sequence: 6 givenname: Marco orcidid: 0000-0001-7244-4389 surname: Brucale fullname: Brucale, Marco – sequence: 7 givenname: Sara surname: Tassinari fullname: Tassinari, Sara – sequence: 8 givenname: Federico orcidid: 0000-0003-2157-8330 surname: Romano fullname: Romano, Federico – sequence: 9 givenname: Giuseppe orcidid: 0000-0002-8031-1102 surname: Ricci fullname: Ricci, Giuseppe – sequence: 10 givenname: Stefania orcidid: 0000-0003-2257-2053 surname: Biffi fullname: Biffi, Stefania |
| BookMark | eNp1Uk1v1DAUjFARLaVnrpa4cNmukzj2hgPSatUtkSq6Eh9X68V5Ka4cu9jOlv0V_GWcbQV0BfbBT_bMPM3zvMyOrLOYZa9zel6WNZ0rsAp9yFkuWE7ps-ykoKKYcV6zo7_q4-wshFuaVlnmgosX2XHJqRC8rE6ynxsDEQ1GslRRbyFqZ4m25HoLXoMlq30PsgxKRwzvUhEwhAFtJK4nl5umaedN0wAB25HNmk3cTwMYQy5-RA8KjRkNePIVg1YGA2l35CNYN1sbd09Wu-gGjH5HlhbMLujwKnvegwl49nieZl_WF59XH2ZX15fNank1U4xXcQbJ74Jh1fUdZ6xtFxz7AkoKgiETlRC46JVStMW2LhjvFGUlFnUBVRqBqGh5mr1_0L0b2wE7lQx5MPLO6wH8TjrQ8umL1d_kjdvKmlWMi0ng7aOAd99HDFEOOkx2waIbgyxEXiwqzuo8Qd8cQG_d6JPhPSovqvQV_A_qBgxKbXs3zW8SlUvBqoSkuUio83-g0u5w0Crlo9fp_glh_kBQ3oXgsf_tMadyipE8iFFiVAeM9Pf7YKRW2vyX9wu4Oc2C |
| CitedBy_id | crossref_primary_10_3390_cancers16213583 crossref_primary_10_3390_cancers16203533 crossref_primary_10_1096_fj_202402499R crossref_primary_10_3389_fbioe_2023_1172457 crossref_primary_10_1002_jex2_171 crossref_primary_10_1016_j_critrevonc_2024_104465 |
| Cites_doi | 10.1038/nrc.2016.113 10.1063/1.1143970 10.1126/sciadv.abg5283 10.1016/j.isci.2021.102676 10.1002/1878-0261.13110 10.1007/s10555-018-9755-8 10.3233/CBM-160614 10.1158/1078-0432.CCR-16-3272 10.1371/journal.pone.0003694 10.2174/1381612043384682 10.3390/ijms21155195 10.1182/blood.V94.11.3791 10.1182/bloodadvances.2020003410 10.1007/s00404-017-4529-z 10.7150/thno.64252 10.1159/000499958 10.1182/bloodadvances.2016000059 10.3390/cells10030544 10.1016/j.tmrv.2020.09.005 10.1182/bloodadvances.2020001758 10.3390/cancers12082063 10.3892/or.2016.4898 10.1371/journal.pone.0049726 10.3389/fonc.2020.00374 10.1021/acs.analchem.9b05716 10.1080/20013078.2019.1697028 10.1111/cas.12987 10.3390/ijms22020790 10.1080/20013078.2018.1535750 10.1158/1055-9965.EPI-14-0146 10.5772/intechopen.80924 10.1182/blood-2012-06-438598 10.7150/ijbs.19776 10.1002/cncr.27772 10.1053/j.seminoncol.2014.04.004 10.1161/ATVBAHA.120.314644 10.3389/fonc.2021.643815 10.2478/s11534-011-0096-2 10.1016/j.ajog.2013.07.016 10.1186/s12885-015-1304-z 10.21037/tcr-21-1946 10.1126/science.1181928 10.1038/s41467-017-00411-z 10.1002/iid3.66 10.1056/NEJMoa1110352 10.3389/fonc.2018.00107 10.1021/ja104052c |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2022 MDPI AG 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2022 by the authors. 2022 |
| Copyright_xml | – notice: COPYRIGHT 2022 MDPI AG – notice: 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2022 by the authors. 2022 |
| DBID | AAYXX CITATION 3V. 7T5 7TO 7XB 8FE 8FH 8FK 8G5 ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU COVID DWQXO GNUQQ GUQSH H94 HCIFZ LK8 M2O M7P MBDVC PHGZM PHGZT PIMPY PKEHL PQEST PQGLB PQQKQ PQUKI PRINS Q9U 7X8 5PM |
| DOI | 10.3390/cancers14174100 |
| DatabaseName | CrossRef ProQuest Central (Corporate) Immunology Abstracts Oncogenes and Growth Factors Abstracts ProQuest Central (purchase pre-March 2016) ProQuest SciTech Collection ProQuest Natural Science Collection ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Research Library ProQuest Central ProQuest Central UK/Ireland ProQuest Central Essentials ProQuest SciTech Premium Collection Natural Science Collection Biological Science Collection ProQuest Central Natural Science Collection ProQuest One Coronavirus Research Database ProQuest Central Korea ProQuest Central Student ProQuest Research Library AIDS and Cancer Research Abstracts SciTech Premium Collection Biological Sciences Research Library Biological Science Database Research Library (Corporate) ProQuest Central Premium ProQuest One Academic (New) Publicly Available Content Database ProQuest One Academic Middle East (New) ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China ProQuest Central Basic MEDLINE - Academic PubMed Central (Full Participant titles) |
| DatabaseTitle | CrossRef Publicly Available Content Database Research Library Prep ProQuest Central Student Oncogenes and Growth Factors Abstracts ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College Research Library (Alumni Edition) ProQuest Natural Science Collection ProQuest Central China ProQuest Central ProQuest One Applied & Life Sciences Natural Science Collection ProQuest Central Korea Biological Science Collection AIDS and Cancer Research Abstracts ProQuest Research Library ProQuest Central (New) ProQuest Biological Science Collection ProQuest Central Basic ProQuest One Academic Eastern Edition Coronavirus Research Database Biological Science Database ProQuest SciTech Collection ProQuest One Academic UKI Edition Immunology Abstracts ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | CrossRef Publicly Available Content Database MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: BENPR name: ProQuest Central url: http://www.proquest.com/pqcentral?accountid=15518 sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| EISSN | 2072-6694 |
| ExternalDocumentID | PMC9454670 A745271017 10_3390_cancers14174100 |
| GeographicLocations | Italy United States--US |
| GeographicLocations_xml | – name: Italy – name: United States--US |
| GrantInformation_xml | – fundername: IRCSS Burlo Garofolo – fundername: Ministry of Health |
| GroupedDBID | --- 53G 5VS 8FE 8FH 8G5 AADQD AAFWJ AAYXX ABDBF ABUWG ACUHS ADBBV AFKRA AFZYC ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BBNVY BCNDV BENPR BHPHI BPHCQ CCPQU CITATION DIK DWQXO E3Z EBD ESX GNUQQ GUQSH GX1 HCIFZ HYE IAO IHR ITC KQ8 LK8 M2O M48 M7P MODMG M~E OK1 P6G PGMZT PHGZM PHGZT PIMPY PQQKQ PROAC RPM TUS PMFND 3V. 7T5 7TO 7XB 8FK COVID H94 MBDVC PKEHL PQEST PQGLB PQUKI PRINS Q9U 7X8 PUEGO 5PM |
| ID | FETCH-LOGICAL-c465t-a10084e5dfd644bb86ef2a30a74e47577e8fccc0beb9246dc043e292a53177503 |
| IEDL.DBID | BENPR |
| ISSN | 2072-6694 |
| IngestDate | Thu Aug 21 18:09:13 EDT 2025 Fri Sep 05 12:03:52 EDT 2025 Fri Jul 25 11:58:33 EDT 2025 Tue Jun 17 22:22:11 EDT 2025 Tue Jun 10 21:20:42 EDT 2025 Tue Jul 01 01:20:54 EDT 2025 Thu Apr 24 23:09:06 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 17 |
| Language | English |
| License | https://creativecommons.org/licenses/by/4.0 Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c465t-a10084e5dfd644bb86ef2a30a74e47577e8fccc0beb9246dc043e292a53177503 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| ORCID | 0000-0003-2157-8330 0000-0003-2257-2053 0000-0001-7244-4389 0000-0001-7910-1274 0000-0001-5793-7206 0000-0002-7823-8719 0000-0002-8031-1102 |
| OpenAccessLink | https://www.proquest.com/docview/2711257636?pq-origsite=%requestingapplication%&accountid=15518 |
| PMID | 36077635 |
| PQID | 2711257636 |
| PQPubID | 2032421 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_9454670 proquest_miscellaneous_2712856491 proquest_journals_2711257636 gale_infotracmisc_A745271017 gale_infotracacademiconefile_A745271017 crossref_primary_10_3390_cancers14174100 crossref_citationtrail_10_3390_cancers14174100 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 20220824 |
| PublicationDateYYYYMMDD | 2022-08-24 |
| PublicationDate_xml | – month: 8 year: 2022 text: 20220824 day: 24 |
| PublicationDecade | 2020 |
| PublicationPlace | Basel |
| PublicationPlace_xml | – name: Basel |
| PublicationTitle | Cancers |
| PublicationYear | 2022 |
| Publisher | MDPI AG MDPI |
| Publisher_xml | – name: MDPI AG – name: MDPI |
| References | Brass (ref_5) 2016; 1 Cho (ref_14) 2012; 120 Fowlkes (ref_8) 2021; 11 Fagotti (ref_26) 2013; 209 Fullard (ref_36) 2004; 10 Perales (ref_13) 2022; 12 Cho (ref_17) 2021; 5 ref_35 ref_11 Adjei (ref_24) 2015; 3 Klapetek (ref_29) 2012; 10 Klotz (ref_2) 2017; 296 Valkonen (ref_40) 2019; 46 Bortot (ref_27) 2021; 15 Saha (ref_18) 2021; 7 Davis (ref_37) 2014; 41 Giusti (ref_4) 2019; 14 ref_38 Kim (ref_42) 2016; 107 Maouia (ref_7) 2020; 34 Kopetz (ref_9) 2018; 37 Lam (ref_20) 2018; 8 Stone (ref_41) 2012; 366 Shen (ref_34) 2021; 10 Haemmerle (ref_12) 2017; 8 Witwer (ref_48) 2018; 7 Hu (ref_16) 2017; 23 Boilard (ref_44) 2010; 327 French (ref_46) 2020; 4 Zhu (ref_32) 2010; 132 Khorana (ref_22) 2013; 119 ref_47 ref_45 Hutter (ref_28) 1993; 64 Miao (ref_33) 2016; 17 ref_43 Ishikawa (ref_15) 2016; 36 Templeton (ref_21) 2014; 23 ref_3 Gkolfinopoulos (ref_10) 2020; 10 Tao (ref_25) 2017; 13 Puhm (ref_39) 2021; 41 Karnezis (ref_1) 2017; 17 Tian (ref_31) 2019; 9 Ridolfi (ref_30) 2020; 92 Malacrida (ref_19) 2021; 24 Heijnen (ref_23) 1999; 94 ref_6 |
| References_xml | – volume: 17 start-page: 65 year: 2017 ident: ref_1 article-title: The Disparate Origins of Ovarian Cancers: Pathogenesis and Prevention Strategies publication-title: Nat. Cancer doi: 10.1038/nrc.2016.113 – volume: 64 start-page: 1868 year: 1993 ident: ref_28 article-title: Calibration of Atomic-force Microscope Tips publication-title: Rev. Sci. Instrum. doi: 10.1063/1.1143970 – volume: 7 start-page: 5283 year: 2021 ident: ref_18 article-title: Human Tumor Microenvironment Chip Evaluates the Consequences of Platelet Extravasation and Combinatorial Antitumor-Antiplatelet Therapy in Ovarian Cancer publication-title: Sci. Adv. doi: 10.1126/sciadv.abg5283 – volume: 24 start-page: 102676 year: 2021 ident: ref_19 article-title: A Human Multi-Cellular Model Shows How Platelets Drive Production of Diseased Extracellular Matrix and Tissue Invasion publication-title: iScience doi: 10.1016/j.isci.2021.102676 – volume: 15 start-page: 3596 year: 2021 ident: ref_27 article-title: Small Extracellular Vesicles from Malignant Ascites of Patients with Advanced Ovarian Cancer Provide Insights into the Dynamics of the Extracellular Matrix publication-title: Mol. Oncol. doi: 10.1002/1878-0261.13110 – volume: 37 start-page: 439 year: 2018 ident: ref_9 article-title: Bioactive Lipid Metabolism in Platelet “First Responder” and Cancer Biology publication-title: Cancer Metastasis Rev. doi: 10.1007/s10555-018-9755-8 – volume: 17 start-page: 33 year: 2016 ident: ref_33 article-title: Neutrophil to Lymphocyte Ratio and Platelet to Lymphocyte Ratio Are Predictive of Chemotherapeutic Response and Prognosis in Epithelial Ovarian Cancer Patients Treated with Platinum-Based Chemotherapy publication-title: Cancer Biomark. doi: 10.3233/CBM-160614 – volume: 14 start-page: 145 year: 2019 ident: ref_4 article-title: Clinical Electron Microscopy in the Study of Human Ovarian Tissues publication-title: EuroMediterr. Biomed. J. – volume: 23 start-page: 5611 year: 2017 ident: ref_16 article-title: Role of Platelet-Derived Tgfβ1 in the Progression of Ovarian Cancer publication-title: Clin. Cancer Res. doi: 10.1158/1078-0432.CCR-16-3272 – ident: ref_43 doi: 10.1371/journal.pone.0003694 – volume: 10 start-page: 1567 year: 2004 ident: ref_36 article-title: The Role of the Platelet Glycoprotein IIb/IIIa in Thrombosis and Haemostasis publication-title: Curr. Pharm. Des. doi: 10.2174/1381612043384682 – ident: ref_47 doi: 10.3390/ijms21155195 – volume: 94 start-page: 3791 year: 1999 ident: ref_23 article-title: Activated Platelets Release Two Types of Membrane Vesicles: Microvesicles by Surface Shedding and Exosomes Derived from Exocytosis of Multivesicular Bodies and Alpha-Granules publication-title: Blood doi: 10.1182/blood.V94.11.3791 – volume: 5 start-page: 1947 year: 2021 ident: ref_17 article-title: The Effect of Platelet G Proteins on Platelet Extravasation and Tumor Growth in the Murine Model of Ovarian Cancer publication-title: Blood Adv. doi: 10.1182/bloodadvances.2020003410 – volume: 296 start-page: 1055 year: 2017 ident: ref_2 article-title: Cells of Origin of Ovarian Cancer: Ovarian Surface Epithelium or Fallopian Tube? publication-title: Arch. Gynecol. Obstet. doi: 10.1007/s00404-017-4529-z – volume: 12 start-page: 2150 year: 2022 ident: ref_13 article-title: Exchange of Cellular Components between Platelets and Tumor Cells: Impact on Tumor Cells Behavior publication-title: Theranostics doi: 10.7150/thno.64252 – volume: 46 start-page: 267 year: 2019 ident: ref_40 article-title: Assessment of Time-Dependent Platelet Activation Using Extracellular Vesicles, CD62P Exposure, and Soluble Glycoprotein V Content of Platelet Concentrates with Two Different Platelet Additive Solutions publication-title: Transfus. Med. Hemotherapy doi: 10.1159/000499958 – volume: 1 start-page: 5 year: 2016 ident: ref_5 article-title: Platelets and Hemostasis: A New Perspective on an Old Subject publication-title: Blood Adv. doi: 10.1182/bloodadvances.2016000059 – ident: ref_35 doi: 10.3390/cells10030544 – volume: 34 start-page: 209 year: 2020 ident: ref_7 article-title: The Immune Nature of Platelets Revisited publication-title: Transfus. Med. Rev. doi: 10.1016/j.tmrv.2020.09.005 – volume: 4 start-page: 3011 year: 2020 ident: ref_46 article-title: Platelet-Derived Extracellular Vesicles Infiltrate and Modify the Bone Marrow during Inflammation publication-title: Blood Adv. doi: 10.1182/bloodadvances.2020001758 – ident: ref_11 doi: 10.3390/cancers12082063 – volume: 36 start-page: 787 year: 2016 ident: ref_15 article-title: Platelets Surrounding Primary Tumor Cells Are Related to Chemoresistance publication-title: Oncol. Rep. doi: 10.3892/or.2016.4898 – ident: ref_45 doi: 10.1371/journal.pone.0049726 – volume: 10 start-page: 374 year: 2020 ident: ref_10 article-title: The Emerging Role of Platelets in the Formation of the Micrometastatic Niche: Current Evidence and Future Perspectives publication-title: Front. Oncol. doi: 10.3389/fonc.2020.00374 – volume: 92 start-page: 10274 year: 2020 ident: ref_30 article-title: AFM-Based High-Throughput Nanomechanical Screening of Single Extracellular Vesicles publication-title: Anal. Chem. doi: 10.1021/acs.analchem.9b05716 – volume: 9 start-page: 1697028 year: 2019 ident: ref_31 article-title: Quality and Efficiency Assessment of Six Extracellular Vesicle Isolation Methods by Nano-Flow Cytometry publication-title: J. Extracell. Vesicles doi: 10.1080/20013078.2019.1697028 – volume: 107 start-page: 1173 year: 2016 ident: ref_42 article-title: Ascites Modulates Cancer Cell Behavior, Contributing to Tumor Heterogeneity in Ovarian Cancer publication-title: Cancer Sci. doi: 10.1111/cas.12987 – ident: ref_3 doi: 10.3390/ijms22020790 – volume: 7 start-page: 1535750 year: 2018 ident: ref_48 article-title: Minimal information for studies of extracellular vesicles 2018 (MISEV2018): A position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines publication-title: J. Extracell. Vesicles doi: 10.1080/20013078.2018.1535750 – volume: 23 start-page: 1204 year: 2014 ident: ref_21 article-title: Prognostic Role of Platelet to Lymphocyte Ratio in Solid Tumors: A Systematic Review and Meta-Analysis publication-title: Cancer Epidemiol. Biomark. Prev. doi: 10.1158/1055-9965.EPI-14-0146 – ident: ref_6 doi: 10.5772/intechopen.80924 – volume: 120 start-page: 4869 year: 2012 ident: ref_14 article-title: Platelets Increase the Proliferation of Ovarian Cancer Cells publication-title: Blood doi: 10.1182/blood-2012-06-438598 – volume: 13 start-page: 828 year: 2017 ident: ref_25 article-title: Platelet-Derived Extracellular Vesicles: An Emerging Therapeutic Approach publication-title: Int. J. Biol. Sci. doi: 10.7150/ijbs.19776 – volume: 119 start-page: 648 year: 2013 ident: ref_22 article-title: Incidence and Predictors of Venous Thromboembolism (VTE) among Ambulatory High-Risk Cancer Patients Undergoing Chemotherapy in the United States publication-title: Cancer doi: 10.1002/cncr.27772 – volume: 41 start-page: 378 year: 2014 ident: ref_37 article-title: Platelet Effects on Ovarian Cancer publication-title: Semin. Oncol. doi: 10.1053/j.seminoncol.2014.04.004 – volume: 41 start-page: 87 year: 2021 ident: ref_39 article-title: Platelet Extracellular Vesicles publication-title: Arter. Thromb. Vasc. Biol. doi: 10.1161/ATVBAHA.120.314644 – volume: 11 start-page: 643815 year: 2021 ident: ref_8 article-title: The Provocative Roles of Platelets in Liver Disease and Cancer publication-title: Front. Oncol. doi: 10.3389/fonc.2021.643815 – volume: 10 start-page: 181 year: 2012 ident: ref_29 article-title: Gwyddion: An Open-Source Software for SPM Data Analysis publication-title: Open Phys. doi: 10.2478/s11534-011-0096-2 – volume: 209 start-page: e1 year: 2013 ident: ref_26 article-title: A Multicentric Trial (Olympia-MITO 13) on the Accuracy of Laparoscopy to Assess Peritoneal Spread in Ovarian Cancer publication-title: Am. J. Obstet. Gynecol. doi: 10.1016/j.ajog.2013.07.016 – ident: ref_38 doi: 10.1186/s12885-015-1304-z – volume: 10 start-page: 4440 year: 2021 ident: ref_34 article-title: Effect of Rho GTPase Activating Protein 9 Combined with Preoperative Ratio of Platelet Distribution Width to Platelet Count on Prognosis of Patients with Serous Ovarian Cancer publication-title: Transl. Cancer Res. doi: 10.21037/tcr-21-1946 – volume: 327 start-page: 580 year: 2010 ident: ref_44 article-title: Platelets Amplify Inflammation in Arthritis via Collagen-Dependent Microparticle Production publication-title: Science doi: 10.1126/science.1181928 – volume: 8 start-page: 310 year: 2017 ident: ref_12 article-title: Platelets Reduce Anoikis and Promote Metastasis by Activating YAP1 Signaling publication-title: Nat. Commun. doi: 10.1038/s41467-017-00411-z – volume: 3 start-page: 133 year: 2015 ident: ref_24 article-title: Understanding the Biosynthesis of Platelets-Derived Extracellular Vesicles publication-title: Immun. Inflamm. Dis. doi: 10.1002/iid3.66 – volume: 366 start-page: 610 year: 2012 ident: ref_41 article-title: Paraneoplastic Thrombocytosis in Ovarian Cancer publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa1110352 – volume: 8 start-page: 107 year: 2018 ident: ref_20 article-title: Platelet Metabolism and Other Targeted Drugs; Potential Impact on Immunotherapy publication-title: Front. Oncol. doi: 10.3389/fonc.2018.00107 – volume: 132 start-page: 12176 year: 2010 ident: ref_32 article-title: Size Differentiation and Absolute Quantification of Gold Nanoparticles via Single Particle Detection with a Laboratory-Built High-Sensitivity Flow Cytometer publication-title: J. Am. Chem. Soc. doi: 10.1021/ja104052c |
| SSID | ssj0000331767 |
| Score | 2.3264165 |
| Snippet | In ovarian cancer, ascites represent the microenvironment in which the platelets extravasate to play their role in the disease progression. We aimed to develop... Platelets play a critical role in coagulation and fibrinolysis processes, but recent literature also indicates their central involvement in immune response,... Simple SummaryPlatelets play a critical role in coagulation and fibrinolysis processes, but recent literature also indicates their central involvement in... |
| SourceID | pubmedcentral proquest gale crossref |
| SourceType | Open Access Repository Aggregation Database Enrichment Source Index Database |
| StartPage | 4100 |
| SubjectTerms | Ascites Ascitic fluid Atomic force microscopy Biomarkers Blood platelets Cancer therapies Cell organelles Centrifugation Chemotherapy Development and progression Extracellular matrix Extracellular vesicles Extravasation Fibrinolysis Flow cytometry Health aspects Hemoglobin Hypotheses Immune response Labeling Medical prognosis Metastases Metastasis Microenvironments Microscopy Ovarian cancer Peritoneum Phenotyping Platelets Polyethylene glycol Surgery |
| SummonAdditionalLinks | – databaseName: Scholars Portal Journals: Open Access dbid: M48 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3fa9RAEF6kgvgi_sTTKiMI-pI2l-xmc4JIOHo2gnqgJ30Ls5sNFtKkvUu191f4LzuTS05zVJ93l2x2Zmfmy0y-EeKl1iTXPEAvj6XxONPkmSJGgipRgX44MXneFsh-io4X8sOJOvnTDqg7wNW10I77SS2W5cHVxfodXfi3jDgJsh9aPp_laiwpuh77hN9vtskiruPrYv3WLIfkKiO9ofe5bt3AM-3a592ayb-c0OyuuNNFj5BsxH1P3HDVfXHrY5cffyB-zUuKHUkUkNi-bxmcVvD5B0FirGDa7gUS8nsUYr6BZMvLCXUB7-dpag7TNEXAKof5TPLaL2dYlnB0Rfvhr_xctgrf3KotpwOzBrLPtTcr658wXTf1mWuWa-ipTh6Kxezo6_TY61oueFZGqvGQuX6kU3mRU6BkTBy5IsDQRy2d1EprFxfWWt84Q8Atyq0vQxdMAqSrrDkl-kjsVXXlHgsIELFQBmOtyQOSRiBFmiZCFZN5pUBnJA76485sx0fObTHKjHAJyyfbkc9IvN4uON9Qcfx76iuWX8Zqw6eD3b8GtDOmu8oSLVWg2RqNxP5gJl0uOxzuNSDrdTOjoTHjtJDe4cV2mFdywVrl6st2ThCrSE7GI6EHmrPdOxN7D0eq0-8twfdEKvJf_pP_P_ypuB3wvxg-mTq5L_aa5aV7RhFSY563mv8bDlkR8w priority: 102 providerName: Scholars Portal |
| Title | Platelet Activation in Ovarian Cancer Ascites: Assessment of GPIIb/IIIa and PF4 in Small Extracellular Vesicles by Nano-Flow Cytometry Analysis |
| URI | https://www.proquest.com/docview/2711257636 https://www.proquest.com/docview/2712856491 https://pubmed.ncbi.nlm.nih.gov/PMC9454670 |
| Volume | 14 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3fb9MwED5tnYR4QeOXCIzJSEjwEpomdpwiIVSqdgvSSgUM9S2yHUdMypKtzYD-FfzL3KVJWCvBS15sK5bvfPedff4O4KWUKNfUV24ace3STZOrs0hhqBJmyguGOk3rBNlZeHrOPy7EYg9m7VsYSqtsbWJtqNPS0Bl535eIDBAcB-H7q2uXqkbR7WpbQkM1pRXSdzXF2D4coEkWXg8OPkxm88_dqYsXoL8M5YbjJ8B4v29ocZerAUdoPqB3brfc066R3k2cvOWJpodwr4GQbLSR-X3Ys8UDuHPWXJI_hN_zHAEkyoONTFu8jF0U7NMPjItVwcb1XNgInR_izLds1JFzsjJjJ_M41v04jhVTRcrmU05jv1yqPGeTXzgfOuqn3FX2za7qnDqm1wyNdOlO8_InG6-r8tJWyzVr-U4ewfl08nV86jZ1F1zDQ1G5igh_uBVpliJa0joKbearwFOSWy6FlDbKjDGethqjtzA1Hg-sP_QV7mdJ96KPoVeUhX0CzFdKZUKrSEp0g6gWCuGmDpWI0MYi2nHgTbvciWlIyak2Rp5gcELySXbk48DrbsDVho_j311fkfwS2qm0Oqp5cIAzI86rZCS5QK1Ck-TA0VZP3GFmu7nVgKTZ4avkrz468KJrppGUtVbY8qbu40ci5MOBA3JLc7q5E7v3dktx8b1m-R5ygU7Me_r_nz-Duz49yPDQ3vEj6FXLG_scYVKljxvdP4b9k8UAv2c8-gPf9xZ9 |
| linkProvider | ProQuest |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1fb9MwED-NTgJeEH9FYICRQPASmiZOnCBNKJSWhm2lgg3tLdiJIyZlyWg7Rj_FvtE-G3dpEtZK8LZn24nlO98f393vAF4IgXRNbWmmPlcmRZpMlfkSXRUvk5YTqDStEmTH3uiAfzp0DzfgoqmFobTKRiZWgjotE3oj79oCLQM0jh3v3clPk7pGUXS1aaEh69YK6XYFMVYXduzoxRm6cLPt6APS-6VtDwf7_ZFZdxkwE-65c1MSvA3XbpqlaBso5Xs6s6VjScE1F64Q2s-SJLGUVuireGlicUfbgS2RewVFAfG712CT0wNKBzbfD8aTL-0rj-XgJE8sMYUcJ7C6CRFzOutxdAV6VFd3SR2uK4X1RM1Lmm94G27VJisLlzx2BzZ0cReu79VB-XtwPsnRYEX6szBpmqWxo4J9_oV-uCxYv9oLC1HZol37loUtGCgrM_ZxEkWqG0WRZLJI2WTIae3XY5nnbPAb90OhBcqVZd_0rMrhY2rBUCmU5jAvz1h_MS-P9Xy6YA2-yn04uBIKPIBOURb6ITBbSpm5SvpCoNpFNpRo3ipPuj7KdLSuDHjTHHec1CDo1Isjj9EZIvrEa_Qx4HW74GSJ__Hvqa-IfjFJBjodWRc44M4IYysOBXeRi1EEGrC1MhNvdLI63HBAXEuUWfyX_w143g7TSsqSK3R5Ws2xfdfjQc8AscI57d4JTXx1pDj6UaGKB9xFpWk9-v_Pn8GN0f7ebrwbjXcew02bikEslLV8Czrz6al-gibaXD2t7wGD71d99f4AhxRRVw |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1fb5RAEN_UNml8Mf6NaNU10egLHgcLCyaNwethsXpe1Jq-4S67xCYU6h213qfwe_mpnOEW7F2ib33eXdgw_5mZ3xDyhHOgq3KFrUImbcw02bIIBYQqQSEcL5JKtQWyk2D_kL098o82yO-uFwbLKjud2CpqVef4j3zgcvAMwDn2gkFhyiKme8mr0-82TpDCTGs3TkOYMQtqt4UbM00eB3pxDuHcfDfdA9o_dd1k_Hm0b5uJA3bOAr-xBULdMO2rQoGfIGUY6MIVniM404z7nOuwyPPckVpC3BKo3GGediNXACdzzAjCc6-QLQ5WHwLBrdfjyfRj_8fH8WBTwJf4Qp4XOYMcCTubDxmEBUPssbtgGtcNxHrR5gUrmFwn14z7SuMlv90gG7q6SbbfmwT9LfJrWoLzCrxA47wbnEaPK_rhB8TkoqKj9i40BsMLPu5LGvfAoLQu6JtpmspBmqaCikrRacLw7KcTUZZ0_BPug2kGrJulX_S8reejckHBQNR2UtbndLRo6hPdzBa0w1q5TQ4vhQJ3yGZVV_ouoa4QovClCDkHEwwsKcDVlYHwQ9Dv4GlZ5EX3ubPcAKLjXI4yg8AI6ZOt0cciz_sDp0sskH9vfYb0y1BL4NcRptkBboZ4W1nMmQ8cDerQIjsrO0G689XljgMyo13m2V9ZsMjjfhlPYsVcpeuzdo8b-gGLhhbhK5zT3x2RxVdXquNvLcJ4xHwwoM69_7_8EdkGEczepZOD--Sqi30hDqhdtkM2m9mZfgDeWiMfGjGg5OtlS94fRzxVmw |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Platelet+Activation+in+Ovarian+Cancer+Ascites%3A+Assessment+of+GPIIb%2FIIIa+and+PF4+in+Small+Extracellular+Vesicles+by+Nano-Flow+Cytometry+Analysis&rft.jtitle=Cancers&rft.au=Bortot%2C+Barbara&rft.au=Mangogna%2C+Alessandro&rft.au=Peacock%2C+Ben&rft.au=Lees%2C+Rebecca&rft.date=2022-08-24&rft.pub=MDPI+AG&rft.eissn=2072-6694&rft.volume=14&rft.issue=17&rft.spage=4100&rft_id=info:doi/10.3390%2Fcancers14174100&rft.externalDBID=HAS_PDF_LINK |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2072-6694&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2072-6694&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2072-6694&client=summon |