Evaluation of a radioimmunoassay for histamine measurement in biologic fluids

• Published in: Journal of allergy and clinical immunology Vol. 82; no. 4; pp. 638 - 646
• Main Authors: McBride, Paul, Bradley, Diane, Kaliner, Michael
• Format: Journal Article
• Language: English
• Published: New York, NY Mosby, Inc 01.10.1988; Elsevier
• Subjects: Biological and medical sciences; Body Fluids - analysis; Chromatography, Thin Layer; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Histamine - analysis; Humans; Hypersensitivity - physiopathology; Immediate hypersensitivity. Allergy. Anaphylaxis, etc; Immunobiology; Male; Radioimmunoassay - methods; Techniques; Assay; Urine; Histamine; Biological fluid; Plasma; Radioimmunoassay; Monoclonal antibody; Acylation; Immunological method
• ISSN: 0091-6749; 1085-8725; 1097-6825
• DOI: 10.1016/0091-6749(88)90977-3

A new radioimmunoassay for the measurement of histamine in biologic fluids was evaluated. Assay selectivity and specificity were achieved by “succinyl-glycinamide derivatization” of histamine in samples to mimic the immunogen used to generate the monoclonal antibody. The assay exhibits a linear response from 0.1 to 5.0 ng/ml of histamine and the monoclonal antibody used has partial recognition of only N-methylhistamine (other than histamine). With minimal modifications, the assay can accurately measure histamine in plasma, urine, and buffer. Normal ranges for human subjects were established: plasma levels are 0.193 ± 0.08 ng/ml (n = 40) and urine levels are 20.9 ± 11.2 μg histamine/gm creatinine (n = 10).