The association between semaphorin 3A levels and gluten-free diet in patients with celiac disease

• Published in: Clinical immunology (Orlando, Fla.) Vol. 184; pp. 73 - 76
• Main Authors: Kessel, Aharon, Lin, Chen, Vadasz, Zahava, Peri, Regina, Eiza, Nasren, Berkowitz, Drora
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2017
• Subjects: Adolescent; Allergy and Immunology; Autoantibodies - immunology; Case-Control Studies; CD4-Positive T-Lymphocytes - immunology; CD4-Positive T-Lymphocytes - metabolism; Celiac disease; Celiac Disease - diet therapy; Celiac Disease - immunology; Celiac Disease - metabolism; Child; Child, Preschool; Diet, Gluten-Free; Female; Gliadin - immunology; Gluten free diet; GTP-Binding Proteins - immunology; Humans; Immunoglobulin A - immunology; Immunoglobulin G - immunology; Interleukin-2 Receptor alpha Subunit - immunology; Male; Semaphorin 3A; Semaphorin-3A - metabolism; T regulatory cells; Transglutaminases - immunology; Gluten free diet; Celiac disease; T regulatory cells; Semaphorin 3A
• ISSN: 1521-6616; 1521-7035; 1521-7035
• DOI: 10.1016/j.clim.2017.05.010

Celiac disease (CD) is an inflammatory disease affecting the small intestine. We aim to assess serum level and expression of semaphorin 3A (Sema3A) on T regulatory (Treg) cells in CD patients. Twenty-six newly diagnosed celiac patients, 13 celiac patients on a gluten-free diet and 16 healthy controls included in the study. Sema3A protein level in the serum of celiac patients was significantly higher compared to healthy group (7.17±1.8ng/ml vs. 5.67±1.5ng/ml, p=0.012). Sema3A expression on Treg cells was statistically lower in celiac patients compared to healthy subjects (p=0.009) and significantly lower in celiac patients compared to celiac patients on gluten free diet (p=0.04). Negative correlation was found between Sema3A on Teg cells and the level of IgA anti-tTG antibodies (r=−0.346, p<0.01) and anti-DGP (r=−0.448, p<0.01). This study suggests involvement of the Sema3A in the pathogenesis of CD. •Serum level of Sema3A taken from diagnosed celiac without gluten-free diet was higher significantly than healthy controls.•Sema3A on CD4+CD25high T cells taken from celiac patients without gluten-free diet was lower compared to healthy controls.•Sema3A on CD4+CD25high T cells from celiac patients exposed to gluten was lower compared to those under gluten-free diet.•Sema3A expression on CD4+CD25high T cells taken from gluten-free diet celiac patients was similar to healthy controls.•Sema3A expression on CD4+CD25high negatively correlated the production of anti-TTG and anti-GDP.