Act1, an NF-κ B-activating Protein

Use of an NF-κ B-dependent selectable marker facilitated the isolation of a cell line containing a cDNA encoding Act1, an NF-κ B activator. Act1 associates with and activates Iκ B kinase (IKK), leading to the liberation of NF-κ B from its complex with NF-κ B. Many signaling pathways that liberate NF...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 97; no. 19; pp. 10489 - 10493
Main Authors Li, Xiaoxia, Commane, Mairead, Nie, Huiqin, Hua, Xianxin, Chatterjee-Kishore, Moitreyee, Wald, David, Haag, Michael, Stark, George R.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 12.09.2000
National Acad Sciences
The National Academy of Sciences
Subjects
3T3 cells
Activating transcription factors
Adaptor Proteins, Signal Transducing
Amino Acid Sequence
Biological Sciences
Carrier Proteins
CDNA libraries
Cell Line
Cell lines
Cellular immunity
Complementary DNA
DNA, Complementary
Enzyme Activation
HEK293 cells
Helix-Loop-Helix Motifs
Humans
Interleukin-1 - metabolism
JNK Mitogen-Activated Protein Kinases
Kidney cells
Mitogen-Activated Protein Kinases - metabolism
Molecular Sequence Data
NF-kappa B - metabolism
Proteins
Recombinant Proteins - metabolism
Sequence Homology, Nucleic Acid
Trans-Activators - chemistry
Trans-Activators - genetics
Trans-Activators - metabolism
Transcription factors
Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
Online AccessGet full text
ISSN0027-8424
1091-6490
DOI10.1073/pnas.160265197

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Summary:Use of an NF-κ B-dependent selectable marker facilitated the isolation of a cell line containing a cDNA encoding Act1, an NF-κ B activator. Act1 associates with and activates Iκ B kinase (IKK), leading to the liberation of NF-κ B from its complex with NF-κ B. Many signaling pathways that liberate NF-κ B also activate activating transcription factor (ATF) and activator protein 1 (AP-1) through Jun kinase (JNK). Act1 also activates JNK, suggesting that it might be part of a multifunctional complex involved in the activation of both NF-κ B and JNK. Act1 fails to activate NF-κ B in an IL-1-unresponsive mutant cell line in which all known signaling components are present, suggesting that it interacts with an unknown component in IL-1 signaling.
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To whom reprint requests should be addressed. E-mail: starkg@ccf.org.
Contributed by George R. Stark
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.160265197