Event‐free survival of infants and toddlers enrolled in the HR‐NBL‐1/SIOPEN trial is associated with the level of neuroblastoma mRNAs at diagnosis

• Published in: Pediatric blood & cancer Vol. 65; no. 7; pp. e27052 - n/a
• Main Authors: Corrias, Maria V., Parodi, Stefano, Tchirkov, Andrei, Lammens, Tim, Vicha, Ales, Pasqualini, Claudia, Träger, Catarina, Yáñez, Yania, Dallorso, Sandro, Varesio, Luigi, Luksch, Roberto, Laureys, Genevieve, Valteau‐Couanet, Dominique, Canete, Adela, Pöetschger, Ulrike, Ladenstein, Ruth, Burchill, Susan A.
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.07.2018; Wiley
• Subjects: Area Under Curve; Biomarkers, Tumor - analysis; Bone marrow; Cancer; Diagnosis; Female; Hematology; Homeobox; Homeodomain Proteins - analysis; Homeodomain Proteins - biosynthesis; Humans; Hydroxylase; Infant; Infant, Newborn; Infants; Kaplan-Meier Estimate; Life Sciences; Male; Neuroblastoma; Neuroblastoma - metabolism; Neuroblastoma - mortality; Neurons and Cognition; Oncology; outcome; Pediatrics; Peripheral blood; Phox2b protein; Polymerase chain reaction; Preschool children; Prognosis; Progression-Free Survival; Proportional Hazards Models; Reverse transcription; Ribonucleic acid; RNA; RNA, Messenger - analysis; ROC Curve; RTqPCR; Sensitivity and Specificity; Survival; toddlers; Transcription Factors - analysis; Transcription Factors - biosynthesis; Tyrosine 3-monooxygenase; Tyrosine 3-Monooxygenase - analysis; Tyrosine 3-Monooxygenase - biosynthesis; neuroblastoma; RTqPCR; toddlers; infants; outcome
• ISSN: 1545-5009; 1545-5017; 1545-5017
• DOI: 10.1002/pbc.27052

Background The purpose of this study was to evaluate whether levels of neuroblastoma mRNAs in bone marrow and peripheral blood from stage M infants (≤12 months of age at diagnosis, MYCN amplified) and toddlers (between 12 and 18 months, any MYCN status) predict event‐free survival (EFS). Methods Bone marrow aspirates and peripheral blood samples from 97 infants/toddlers enrolled in the European High‐Risk Neuroblastoma trial were collected at diagnosis in PAXgene™ blood RNA tubes. Samples were analyzed by reverse transcription quantitative polymerase chain reaction according to standardized procedures. Results Bone marrow tyrosine hydroxylase (TH) or paired‐like homeobox 2b (PHOX2B) levels in the highest tertile were associated with worse EFS; hazard ratios, adjusted for age and MYCN status, were 1.5 and 1.8 respectively. Expression of both TH and PHOX2B in the highest tertile predicted worse outcome (p = 0.015), and identified 20 (23%) infants/toddlers with 5‐year EFS of 20% (95%CI: 4%–44%). Prognostic significance was maintained after adjusting for over‐fitting bias (p = 0.038), age and MYCN status. In peripheral blood, PHOX2B levels in the highest tertile predicted a two‐fold increased risk of an event (p = 0.032), and identified 23 (34%) infants/toddlers with 5‐year EFS of 29% (95%CI: 12%–48%). Time‐dependent receiver operating characteristic analysis confirmed the prognostic value of combined TH and PHOX2B in bone marrow and of PHOX2B in peripheral blood during the first year of follow‐up. Conclusions High levels of bone marrow TH and PHOX2B and of peripheral blood PHOX2B at diagnosis allow early identification of a group of high‐risk infant and toddlers with neuroblastoma who may be candidates for alternative treatments. Integration with additional biomarkers, as well as validation in additional international trials is warranted.