Single-laboratory Validation Study and Surveillance Using an Improved Multiresidue Analytical Method for Veterinary Drugs in Livestock Products by LC-MS/MS

• Published in: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 64; no. 1; pp. 53 - 60
• Main Authors: Nakajima, Takayuki, Nagano, Chieko, Yoshikawa, Souichi, Matsushima, Yoko, Sasamoto, Takeo, Hayashi, Hiroshi, Koike, Hiroshi, Hayashi, Momoka, Ohba, Yumi, Otsuka, Kenji, Kanda, Maki
• Format: Journal Article
• Language: English; Japanese
• Published: Japan Japanese Society for Food Hygiene and Safety 25.02.2023; Japan Science and Technology Agency
• Subjects: Acetonitrile; Analytical chemistry; Analytical methods; Animals; Anti-Bacterial Agents; Aquatic birds; Chickens; Chromatography, Liquid; Coccidiostats; Criteria; Dehydration; Drugs; Eggs; LC-MS/MS; Livestock; livestock products; macrolides; Magnesium; Magnesium sulfate; Milk; multiclass analysis; Muscles; Residues; single-laboratory validation study; Sodium; Sodium chloride; Sodium citrate; Sulfonamides; surveillance; Tandem Mass Spectrometry; Veterinary Drugs; Veterinary medicine; Waterfowl; LC-MS/MS; multiclass analysis; livestock products; macrolides; coccidiostats; single-laboratory validation study; surveillance
• ISSN: 0015-6426; 1882-1006
• DOI: 10.3358/shokueishi.64.53

A method for the rapid analysis of multiclass residual veterinary drugs in poultry muscle, egg, and raw milk was validated in accordance with Japanese guidelines. Using LC-MS/MS, 20 veterinary drugs, including sulfonamides, coccidiostats, and macrolides were analyzed in one injection. Analytes were extracted from the samples with acetonitrile and then dehydrated and salted out using magnesium sulfate, trisodium citrate, and sodium chloride. This method was assessed by performing recovery tests of chicken muscle, duck muscle, egg, and raw milk spiked with 20 new target analytes at concentrations of 10 and 100 µg/kg. According to this method, 17 out of 20 target analytes satisfied the guideline criteria in chicken muscle and duck muscle, and all 20 target analytes met the criteria in egg and raw milk. The limit of quantification was less than MRLs for all analytes. Residues were detected in 4 out of 99 samples and analyzed using the validated method, finding that the levels of all residues were lower than the limits of quantification. These results suggest that continuous monitoring for a new trend of veterinary drugs is necessary.