Lovastatin stimulates human vascular smooth muscle cell expression of bone morphogenetic protein-2, a potent inhibitor of low-density lipoprotein-stimulated cell growth
Bone morphogenetic proteins (BMPs) stimulate ectopic bone formation in skeletal muscle. Here we show that human vascular smooth muscle cells (VSMC) abundantly express mRNA encoding for BMP receptor type II, BMP-2, and BMP-7 proteins. Treatment with the 3-hydroxy-3-methylglutaryl coenzyme A inhibitor...
Saved in:
| Published in | Biochemical and biophysical research communications Vol. 302; no. 1; pp. 67 - 72 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Elsevier Inc
28.02.2003
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 0006-291X 1090-2104 |
| DOI | 10.1016/S0006-291X(03)00109-8 |
Cover
| Abstract | Bone morphogenetic proteins (BMPs) stimulate ectopic bone formation in skeletal muscle. Here we show that human vascular smooth muscle cells (VSMC) abundantly express mRNA encoding for BMP receptor type II, BMP-2, and BMP-7 proteins. Treatment with the 3-hydroxy-3-methylglutaryl coenzyme A inhibitor lovastatin (34
μM) increased BMP-2 gene transcription >14-fold as measured by real-time PCR analysis (
P<0.05 vs. solvent control). Moreover, VSMC proliferation stimulated with native low-density lipoprotein (100
μg of protein/mL) was prevented by either human recombinant BMP-2 or BMP-7 at concentrations of 100
ng/mL (
P<0.05). Both BMPs also inhibited basal cell proliferation (
P<0.05). Induction of BMPs and subsequent inhibition of VSMC growth and/or induction of vascular bone formation could contribute to the mechanisms by which statins increase plaque stability in patients with coronary atherosclerosis. |
|---|---|
| AbstractList | Bone morphogenetic proteins (BMPs) stimulate ectopic bone formation in skeletal muscle. Here we show that human vascular smooth muscle cells (VSMC) abundantly express mRNA encoding for BMP receptor type II, BMP-2, and BMP-7 proteins. Treatment with the 3-hydroxy-3-methylglutaryl coenzyme A inhibitor lovastatin (34 microM) increased BMP-2 gene transcription >14-fold as measured by real-time PCR analysis (P<0.05 vs. solvent control). Moreover, VSMC proliferation stimulated with native low-density lipoprotein (100 microg of protein/mL) was prevented by either human recombinant BMP-2 or BMP-7 at concentrations of 100 ng/mL (P<0.05). Both BMPs also inhibited basal cell proliferation (P<0.05). Induction of BMPs and subsequent inhibition of VSMC growth and/or induction of vascular bone formation could contribute to the mechanisms by which statins increase plaque stability in patients with coronary atherosclerosis. Bone morphogenetic proteins (BMPs) stimulate ectopic bone formation in skeletal muscle. Here we show that human vascular smooth muscle cells (VSMC) abundantly express mRNA encoding for BMP receptor type II, BMP-2, and BMP-7 proteins. Treatment with the 3-hydroxy-3-methylglutaryl coenzyme A inhibitor lovastatin (34 mu M) increased BMP-2 gene transcription >14-fold as measured by real-time PCR analysis (P0.05 vs. solvent control). Moreover, VSMC proliferation stimulated with native low-density lipoprotein (100 mu g of protein/mL) was prevented by either human recombinant BMP-2 or BMP-7 at concentrations of 100ng/mL (P0.05). Both BMPs also inhibited basal cell proliferation (P0.05). Induction of BMPs and subsequent inhibition of VSMC growth and/or induction of vascular bone formation could contribute to the mechanisms by which statins increase plaque stability in patients with coronary atherosclerosis. Bone morphogenetic proteins (BMPs) stimulate ectopic bone formation in skeletal muscle. Here we show that human vascular smooth muscle cells (VSMC) abundantly express mRNA encoding for BMP receptor type II, BMP-2, and BMP-7 proteins. Treatment with the 3-hydroxy-3-methylglutaryl coenzyme A inhibitor lovastatin (34 microM) increased BMP-2 gene transcription >14-fold as measured by real-time PCR analysis (P<0.05 vs. solvent control). Moreover, VSMC proliferation stimulated with native low-density lipoprotein (100 microg of protein/mL) was prevented by either human recombinant BMP-2 or BMP-7 at concentrations of 100 ng/mL (P<0.05). Both BMPs also inhibited basal cell proliferation (P<0.05). Induction of BMPs and subsequent inhibition of VSMC growth and/or induction of vascular bone formation could contribute to the mechanisms by which statins increase plaque stability in patients with coronary atherosclerosis.Bone morphogenetic proteins (BMPs) stimulate ectopic bone formation in skeletal muscle. Here we show that human vascular smooth muscle cells (VSMC) abundantly express mRNA encoding for BMP receptor type II, BMP-2, and BMP-7 proteins. Treatment with the 3-hydroxy-3-methylglutaryl coenzyme A inhibitor lovastatin (34 microM) increased BMP-2 gene transcription >14-fold as measured by real-time PCR analysis (P<0.05 vs. solvent control). Moreover, VSMC proliferation stimulated with native low-density lipoprotein (100 microg of protein/mL) was prevented by either human recombinant BMP-2 or BMP-7 at concentrations of 100 ng/mL (P<0.05). Both BMPs also inhibited basal cell proliferation (P<0.05). Induction of BMPs and subsequent inhibition of VSMC growth and/or induction of vascular bone formation could contribute to the mechanisms by which statins increase plaque stability in patients with coronary atherosclerosis. Bone morphogenetic proteins (BMPs) stimulate ectopic bone formation in skeletal muscle. Here we show that human vascular smooth muscle cells (VSMC) abundantly express mRNA encoding for BMP receptor type II, BMP-2, and BMP-7 proteins. Treatment with the 3-hydroxy-3-methylglutaryl coenzyme A inhibitor lovastatin (34 μM) increased BMP-2 gene transcription >14-fold as measured by real-time PCR analysis ( P<0.05 vs. solvent control). Moreover, VSMC proliferation stimulated with native low-density lipoprotein (100 μg of protein/mL) was prevented by either human recombinant BMP-2 or BMP-7 at concentrations of 100 ng/mL ( P<0.05). Both BMPs also inhibited basal cell proliferation ( P<0.05). Induction of BMPs and subsequent inhibition of VSMC growth and/or induction of vascular bone formation could contribute to the mechanisms by which statins increase plaque stability in patients with coronary atherosclerosis. |
| Author | Ortmann, Jana Doerflinger, Tim Emmanuele, Luca Traupe, Tobias Barton, Matthias |
| Author_xml | – sequence: 1 givenname: Luca surname: Emmanuele fullname: Emmanuele, Luca – sequence: 2 givenname: Jana surname: Ortmann fullname: Ortmann, Jana – sequence: 3 givenname: Tim surname: Doerflinger fullname: Doerflinger, Tim – sequence: 4 givenname: Tobias surname: Traupe fullname: Traupe, Tobias – sequence: 5 givenname: Matthias surname: Barton fullname: Barton, Matthias email: barton@usz.ch |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/12593849$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkctu1DAUhi1URKeFRwB5hUAi4NsksVggVHGTRmIBSOwsxz5pjBI72E5L34jHxOm0s2Azq-PL95_z6_xn6MQHDwg9peQ1JbR-840QUldM0p8vCH9JCCWyah-gTamkYpSIE7Q5IKfoLKVfBaKilo_QKWVbyVshN-jvLlzplHV2HqfspmXUGRIelkl7XH5MeYg4TSHkAU9LMiNgA-OI4c8cISUXPA497oo5PIU4D-ESPGRn8BxDBucr9gprPJezz9j5wXUuh7hqxnBdWfDJ5Rs8ujncCw427H7SZQzXeXiMHvZ6TPDkrp6jHx8_fL_4XO2-fvpy8X5XGbFtc2VtWQDn0PetbHVnTA1dA5x3jDAg0nSS0abRHbcCTG_rrm36cgHKoK0Zs_wcPd_3LXZ-L5CymlxafWgPYUmq4UQI0dZHQSq5FJyv4LM7cOkmsGqObtLxRt2HUIC3e8DEkFKEXhm3JhJ8jtqNihK1Rq5uI1drnopwdRu5aot6-5_6MOCI7t1eB2WbVw6iSsaBN2BdBJOVDe5Ih3_ApslM |
| CitedBy_id | crossref_primary_10_1097_CD9_0000000000000050 crossref_primary_10_1097_MOL_0b013e3283613a94 crossref_primary_10_1165_rcmb_2011_0411OC crossref_primary_10_1016_j_bbrc_2004_11_160 crossref_primary_10_1097_00000441_200608000_00001 crossref_primary_10_1007_s00774_014_0638_y crossref_primary_10_1016_j_jbspin_2007_12_008 crossref_primary_10_1053_j_gastro_2008_02_059 crossref_primary_10_1186_s40360_022_00600_7 crossref_primary_10_1007_s10856_015_5650_9 crossref_primary_10_1097_BRS_0b013e31817c561b crossref_primary_10_1128_MCB_00989_10 crossref_primary_10_1161_HYPERTENSIONAHA_107_089995 crossref_primary_10_1016_S8756_3282_03_00239_4 crossref_primary_10_1016_j_taap_2005_12_015 crossref_primary_10_1016_j_bbrc_2005_06_140 crossref_primary_10_1161_ATVBAHA_118_311880 crossref_primary_10_1016_j_cjca_2016_02_062 crossref_primary_10_1016_j_jvs_2005_08_008 crossref_primary_10_1016_j_lfs_2005_09_026 crossref_primary_10_1253_circj_72_807 crossref_primary_10_1359_jbmr_080603 crossref_primary_10_1111_j_1365_2613_2010_00705_x crossref_primary_10_1016_j_atherosclerosis_2008_01_017 crossref_primary_10_1097_00029330_200803010_00021 crossref_primary_10_1017_S0007114507744434 crossref_primary_10_1074_jbc_M110_202929 crossref_primary_10_1016_j_injury_2015_08_034 crossref_primary_10_1634_stemcells_2007_0744 crossref_primary_10_1016_j_rhum_2007_12_014 crossref_primary_10_1111_j_1600_6143_2005_00807_x crossref_primary_10_1517_13543784_16_6_803 crossref_primary_10_4049_jimmunol_0802814 |
| Cites_doi | 10.1002/(SICI)1097-4652(200007)184:1<37::AID-JCP4>3.0.CO;2-M 10.1006/bbrc.2001.4987 10.1016/S0891-5849(01)00610-4 10.1016/S0021-9150(00)00708-5 10.1016/0003-2697(85)90442-7 10.1002/jcp.1080 10.1007/BF02018308 10.1172/JCI114485 10.1161/01.ATV.17.4.680 10.1172/JCI116391 10.1161/01.CIR.104.3.249 10.1083/jcb.200201089 10.1097/00004872-200211000-00024 10.1016/S0092-8674(01)00571-2 10.1056/NEJM199901143400207 10.1242/dev.00181 10.1172/JCI119830 10.1161/01.HYP.34.4.987 10.1038/nm1102-1257 10.1126/science.150.3698.893 10.1083/jcb.103.6.2787 10.1007/s004280050289 10.1016/S0002-9149(01)01713-1 10.1161/hq1201.100229 10.1126/science.286.5446.1946 10.1136/hrt.50.2.127 10.1161/hy0202.103473 10.1159/000025634 |
| ContentType | Journal Article |
| Copyright | 2003 Elsevier Science (USA) |
| Copyright_xml | – notice: 2003 Elsevier Science (USA) |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 7QP 7X8 |
| DOI | 10.1016/S0006-291X(03)00109-8 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Calcium & Calcified Tissue Abstracts MEDLINE - Academic |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Calcium & Calcified Tissue Abstracts MEDLINE - Academic |
| DatabaseTitleList | MEDLINE Calcium & Calcified Tissue Abstracts MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Anatomy & Physiology Chemistry Biology |
| EISSN | 1090-2104 |
| EndPage | 72 |
| ExternalDocumentID | 12593849 10_1016_S0006_291X_03_00109_8 S0006291X03001098 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GroupedDBID | --- --K --M -~X .55 .GJ .HR .~1 0R~ 1B1 1CY 1RT 1~. 1~5 23N 3O- 4.4 457 4G. 53G 5GY 5VS 6J9 7-5 71M 8P~ 9JM 9M8 AABNK AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AAXUO AAYJJ ABEFU ABFNM ABFRF ABGSF ABJNI ABMAC ABUDA ABXDB ABYKQ ACDAQ ACGFO ACGFS ACKIV ACNCT ACRLP ADBBV ADEZE ADFGL ADIYS ADMUD ADUVX AEBSH AEFWE AEHWI AEKER AENEX AFFNX AFKWA AFTJW AFXIZ AGHFR AGRDE AGUBO AGYEJ AHHHB AHPSJ AIEXJ AIKHN AITUG AJBFU AJOXV ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC CAG COF CS3 D0L DM4 DOVZS EBS EFBJH EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q G8K GBLVA HLW HVGLF HZ~ IHE J1W K-O KOM L7B LG5 LX2 M41 MO0 MVM N9A O-L O9- OAUVE OHT OZT P-8 P-9 P2P PC. Q38 R2- RIG RNS ROL RPZ SBG SCC SDF SDG SDP SES SEW SPCBC SSU SSZ T5K TWZ UQL WH7 WUQ X7M XPP XSW Y6R ZA5 ZGI ZKB ZMT ~02 ~G- ~KM AAHBH AATTM AAXKI AAYWO AAYXX ABDPE ABWVN ACRPL ACVFH ADCNI ADNMO AEIPS AEUPX AFJKZ AFPUW AGCQF AGQPQ AGRNS AIGII AIIUN AKBMS AKRWK AKYEP ANKPU APXCP BNPGV CITATION SSH CGR CUY CVF ECM EIF NPM PKN 7QP EFKBS 7X8 |
| ID | FETCH-LOGICAL-c458t-dd10933eff898abcc6eb7e33b202e09cb92177ab3d4ecfd6b87fb3de12e8622d3 |
| IEDL.DBID | .~1 |
| ISSN | 0006-291X |
| IngestDate | Fri Sep 05 10:17:33 EDT 2025 Fri Sep 05 11:27:54 EDT 2025 Wed Feb 19 02:36:19 EST 2025 Tue Jul 01 02:41:28 EDT 2025 Thu Apr 24 23:03:55 EDT 2025 Fri Feb 23 02:28:05 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Keywords | Low-density lipoprotein Plaque rupture BMP-statins Thymidine incorporation Reverse transcriptase polymerase chain reaction HMG-CoA reductase inhibitor Transforming growth factor-β Bone Risk factors Cholesterol |
| Language | English |
| License | https://www.elsevier.com/tdm/userlicense/1.0 |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c458t-dd10933eff898abcc6eb7e33b202e09cb92177ab3d4ecfd6b87fb3de12e8622d3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 12593849 |
| PQID | 19394336 |
| PQPubID | 23462 |
| PageCount | 6 |
| ParticipantIDs | proquest_miscellaneous_73044486 proquest_miscellaneous_19394336 pubmed_primary_12593849 crossref_citationtrail_10_1016_S0006_291X_03_00109_8 crossref_primary_10_1016_S0006_291X_03_00109_8 elsevier_sciencedirect_doi_10_1016_S0006_291X_03_00109_8 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2003-02-28 |
| PublicationDateYYYYMMDD | 2003-02-28 |
| PublicationDate_xml | – month: 02 year: 2003 text: 2003-02-28 day: 28 |
| PublicationDecade | 2000 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Biochemical and biophysical research communications |
| PublicationTitleAlternate | Biochem Biophys Res Commun |
| PublicationYear | 2003 |
| Publisher | Elsevier Inc |
| Publisher_xml | – name: Elsevier Inc |
| References | Jeziorska, McCollum, Wooley (BIB5) 1998; 433 Stary (BIB7) 2001; 88 Gong, Slee, Fukai, Rawadi, Roman-Roman, Reginato, Wang, Cundy, Glorieux, Lev, Zacharin, Oexle, Marcelino, Suwairi, Heeger, Sabatakos, Apte, Adkins, Allgrove, Arslan-Kirchner, Batch, Beighton, Black, Boles, Boon, Borrone, Brunner, Carle, Dallapiccola, De Paepe, Floege, Halfhide, Hall, Hennekam, Hirose, Jans, Juppner, Kim, Keppler-Noreuil, Kohlschuetter, LaCombe, Lambert, Lemyre, Letteboer, Peltonen, Ramesar, Romanengo, Somer, Steichen-Gersdorf, Steinmann, Sullivan, Superti-Furga, Swoboda, van den Boogaard, Van Hul, Vikkula, Votruba, Zabel, Garcia, Baron, Olsen, Warman (BIB10) 2001; 107 Parhami, Morrow, Balucan, Leitinger, Watson, Tintut, Berliner, Demer (BIB28) 1997; 17 Bostrom, Watson, Horn, Wortham, Herman, Demer (BIB6) 1993; 91 Ross (BIB1) 1999; 340 Shiomi, Ito, Hirouchi, Enomoto (BIB19) 2001; 157 Libby (BIB2) 2001; 88 Willette, Gu, Lysko, Anderson, Minehart, Yue (BIB14) 1999; 36 Faggiotto, Paoletti (BIB29) 1999; 34 Dhore, Cleutjens, Lutgens, Cleutjens, Geusens, Kitslaar, Tordoir, Spronk, Vermeer, Daemen (BIB15) 2001; 21 Garrett, Boyce, Oreffo, Bonewald, Poser, Mundy (BIB27) 1990; 85 Nakaoka, Gonda, Ogita, Otawara-Hamamoto, Okabe, Kira, Harii, Miyazono, Takuwa, Fujita (BIB17) 1997; 100 Traupe, Lang, Goettsch, Münter, Morawietz, Vetter, Barton (BIB25) 2002; 11 Falk (BIB3) 1983; 50 Kato, Patel, Levasseur, Lobov, Chang, Glass, Hartmann, Li, Brayton, Lang, Karsenty, Chan (BIB9) 2002; 157 Skalli (BIB24) 1986; 103 Miyazono, Kusanagi, Inoue (BIB12) 2001; 187 Corti, Fayad, Fuster, Worthley, Helft, Chesebro, Mercuri, Badimon (BIB18) 2001; 104 Libby, Aikawa (BIB4) 2002; 8 Dorai, Vukicevic, Sampath (BIB16) 2000; 184 Smith, Krohn, Hermanson, Mallia, Gartner, Provenzano, Fujimoto, Goeke, Olson, Klenk (BIB23) 1985; 150 Phillips, Belmonte, Vernochet, Ailhaud, Dani (BIB21) 2001; 284 Mundy, Garrett, Harris, Chan, Chen, Rossini, Boyce, Zhao, Gutierrez (BIB20) 1999; 286 Haudenschild (BIB8) 1990 Locher, Brandes, Vetter, Barton (BIB22) 2002; 39 Délot, Bahamonde, Zhao, Lyons (BIB13) 2003; 130 Urist (BIB11) 1965; 150 Mody, Parhami, Sarafian, Demer (BIB26) 2001; 31 Libby (10.1016/S0006-291X(03)00109-8_BIB4) 2002; 8 Dorai (10.1016/S0006-291X(03)00109-8_BIB16) 2000; 184 Traupe (10.1016/S0006-291X(03)00109-8_BIB25) 2002; 11 Mody (10.1016/S0006-291X(03)00109-8_BIB26) 2001; 31 Libby (10.1016/S0006-291X(03)00109-8_BIB2) 2001; 88 Nakaoka (10.1016/S0006-291X(03)00109-8_BIB17) 1997; 100 Corti (10.1016/S0006-291X(03)00109-8_BIB18) 2001; 104 Locher (10.1016/S0006-291X(03)00109-8_BIB22) 2002; 39 Bostrom (10.1016/S0006-291X(03)00109-8_BIB6) 1993; 91 Jeziorska (10.1016/S0006-291X(03)00109-8_BIB5) 1998; 433 Garrett (10.1016/S0006-291X(03)00109-8_BIB27) 1990; 85 Ross (10.1016/S0006-291X(03)00109-8_BIB1) 1999; 340 Haudenschild (10.1016/S0006-291X(03)00109-8_BIB8) 1990 Urist (10.1016/S0006-291X(03)00109-8_BIB11) 1965; 150 Miyazono (10.1016/S0006-291X(03)00109-8_BIB12) 2001; 187 Stary (10.1016/S0006-291X(03)00109-8_BIB7) 2001; 88 Faggiotto (10.1016/S0006-291X(03)00109-8_BIB29) 1999; 34 Gong (10.1016/S0006-291X(03)00109-8_BIB10) 2001; 107 Phillips (10.1016/S0006-291X(03)00109-8_BIB21) 2001; 284 Willette (10.1016/S0006-291X(03)00109-8_BIB14) 1999; 36 Smith (10.1016/S0006-291X(03)00109-8_BIB23) 1985; 150 Dhore (10.1016/S0006-291X(03)00109-8_BIB15) 2001; 21 Délot (10.1016/S0006-291X(03)00109-8_BIB13) 2003; 130 Mundy (10.1016/S0006-291X(03)00109-8_BIB20) 1999; 286 Falk (10.1016/S0006-291X(03)00109-8_BIB3) 1983; 50 Skalli (10.1016/S0006-291X(03)00109-8_BIB24) 1986; 103 Parhami (10.1016/S0006-291X(03)00109-8_BIB28) 1997; 17 Shiomi (10.1016/S0006-291X(03)00109-8_BIB19) 2001; 157 Kato (10.1016/S0006-291X(03)00109-8_BIB9) 2002; 157 |
| References_xml | – volume: 284 start-page: 478 year: 2001 end-page: 484 ident: BIB21 article-title: Compactin enhances osteogenesis in murine embryonic stem cells publication-title: Biochem. Biophys. Res. Commun. – volume: 286 start-page: 1946 year: 1999 end-page: 1949 ident: BIB20 article-title: Stimulation of bone formation in vitro and in rodents by statins publication-title: Science – start-page: 993 year: 1990 end-page: 1004 ident: BIB8 article-title: Pathogenesis of atherosclerosis: state of the art publication-title: Cardiovasc. Drugs Ther. – volume: 150 start-page: 893 year: 1965 end-page: 899 ident: BIB11 article-title: Bone: formation by autoinduction publication-title: Science – volume: 88 start-page: 3J year: 2001 end-page: 6J ident: BIB2 article-title: What have we learned about the biology of atherosclerosis? The role of inflammation publication-title: Am. J. Cardiol. – volume: 11 start-page: 2239 year: 2002 end-page: 2245 ident: BIB25 article-title: Obesity increases prostanoid-mediated vasosconstriction and vascular thromboxane receptor gene expression publication-title: J. Hypertens. – volume: 50 start-page: 127 year: 1983 end-page: 134 ident: BIB3 article-title: Plaque rupture with severe pre-existing stenosis precipitating coronary thrombosis. Characteristics of coronary atherosclerotic plaques underlying fatal occlusive thrombi publication-title: Br. Heart J. – volume: 103 start-page: 2787 year: 1986 end-page: 2796 ident: BIB24 article-title: A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation publication-title: J. Cell Biol. – volume: 85 start-page: 632 year: 1990 end-page: 639 ident: BIB27 article-title: Oxygen-derived free radicals stimulate osteoclastic bone resorption in rodent bone in vitro and in vivo publication-title: J. Clin. Invest. – volume: 187 start-page: 265 year: 2001 end-page: 276 ident: BIB12 article-title: Divergence and convergence of TGF-β/BMP signaling publication-title: J. Cell Physiol. – volume: 88 start-page: 16E year: 2001 end-page: 19E ident: BIB7 article-title: The development of calcium deposits in atherosclerotic lesions and their persistence after lipid regression publication-title: Am. J. Cardiol. – volume: 8 start-page: 1257 year: 2002 end-page: 1262 ident: BIB4 article-title: Stabilization of atherosclerotic plaques: new mechanisms and clinical targets publication-title: Nat. Med. – volume: 107 start-page: 513 year: 2001 end-page: 523 ident: BIB10 article-title: LDL receptor-related protein 5 (LRP5) affects bone accrual and eye development publication-title: Cell – volume: 433 start-page: 559 year: 1998 end-page: 565 ident: BIB5 article-title: Observations on bone formation and remodelling in advanced atherosclerotic lesions of human carotid arteries publication-title: Virchows Arch. – volume: 157 start-page: 75 year: 2001 end-page: 84 ident: BIB19 article-title: Fibromuscular cap composition is important for the stability of established atherosclerotic plaques in mature WHHL rabbits treated with statins publication-title: Atherosclerosis – volume: 21 start-page: 1998 year: 2001 end-page: 2003 ident: BIB15 article-title: Differential expression of bone matrix regulatory proteins in human atherosclerotic plaques publication-title: Arterioscler. Thromb. Vasc. Biol. – volume: 36 start-page: 120 year: 1999 end-page: 125 ident: BIB14 article-title: BMP-2 gene expression and effects on human vascular smooth muscle cells publication-title: J. Vasc. Res. – volume: 150 start-page: 76 year: 1985 end-page: 85 ident: BIB23 article-title: Measurement of protein using bicinchoninic acid publication-title: Anal. Biochem. – volume: 91 start-page: 1800 year: 1993 end-page: 1809 ident: BIB6 article-title: Bone morphogenetic protein expression in human atherosclerotic lesions publication-title: J. Clin. Invest. – volume: 31 start-page: 509 year: 2001 end-page: 519 ident: BIB26 article-title: Oxidative stress modulates osteoblastic differentiation of vascular and bone cells publication-title: Free Radic. Biol. Med. – volume: 340 start-page: 115 year: 1999 end-page: 126 ident: BIB1 article-title: Atherosclerosis—an inflammatory disease publication-title: N. Engl. J. Med. – volume: 39 start-page: 645 year: 2002 end-page: 650 ident: BIB22 article-title: Native LDL induces proliferation of human vascular smooth muscle cells via redox-mediated activation of ERK 1/2 mitogen-activated protein kinases publication-title: Hypertension – volume: 17 start-page: 680 year: 1997 end-page: 687 ident: BIB28 article-title: Lipid oxidation products have opposite effects on calcifying vascular cell and bone cell differentiation. A possible explanation for the paradox of arterial calcification in osteoporotic patients publication-title: Arterioscler. Thromb. Vasc. Biol. – volume: 34 start-page: 987 year: 1999 end-page: 996 ident: BIB29 article-title: State-of-the-Art lecture. Statins and blockers of the renin–angiotensin system: vascular protection beyond their primary mode of action publication-title: Hypertension – volume: 100 start-page: 2824 year: 1997 end-page: 2832 ident: BIB17 article-title: Inhibition of rat vascular smooth muscle proliferation in vitro and in vivo by bone morphogenetic protein-2 publication-title: J. Clin. Invest. – volume: 157 start-page: 303 year: 2002 end-page: 314 ident: BIB9 article-title: Cbfa1-independent decrease in osteoblast proliferation, osteopenia, and persistant embryonic eye vascularization in mice deficient in Lrp5, a Wnt coreceptor publication-title: J. Cell Biol. – volume: 104 start-page: 249 year: 2001 end-page: 252 ident: BIB18 article-title: Effects of lipid-lowering by simvastatin on human atherosclerotic lesions: a longitudinal study by high-resolution, noninvasive magnetic resonance imaging publication-title: Circulation – volume: 130 start-page: 209 year: 2003 end-page: 220 ident: BIB13 article-title: BMP signaling is required for septation of the outflow tract of the mammalian heart publication-title: Development – volume: 184 start-page: 37 year: 2000 end-page: 45 ident: BIB16 article-title: Bone morphogenetic protein-7 (osteogenic protein-1) inhibits smooth muscle cell proliferation and stimulates the expression of markers that are characteristic of SMC phenotype in vitro publication-title: J. Cell Physiol. – volume: 184 start-page: 37 year: 2000 ident: 10.1016/S0006-291X(03)00109-8_BIB16 article-title: Bone morphogenetic protein-7 (osteogenic protein-1) inhibits smooth muscle cell proliferation and stimulates the expression of markers that are characteristic of SMC phenotype in vitro publication-title: J. Cell Physiol. doi: 10.1002/(SICI)1097-4652(200007)184:1<37::AID-JCP4>3.0.CO;2-M – volume: 284 start-page: 478 year: 2001 ident: 10.1016/S0006-291X(03)00109-8_BIB21 article-title: Compactin enhances osteogenesis in murine embryonic stem cells publication-title: Biochem. Biophys. Res. Commun. doi: 10.1006/bbrc.2001.4987 – volume: 31 start-page: 509 year: 2001 ident: 10.1016/S0006-291X(03)00109-8_BIB26 article-title: Oxidative stress modulates osteoblastic differentiation of vascular and bone cells publication-title: Free Radic. Biol. Med. doi: 10.1016/S0891-5849(01)00610-4 – volume: 157 start-page: 75 year: 2001 ident: 10.1016/S0006-291X(03)00109-8_BIB19 article-title: Fibromuscular cap composition is important for the stability of established atherosclerotic plaques in mature WHHL rabbits treated with statins publication-title: Atherosclerosis doi: 10.1016/S0021-9150(00)00708-5 – volume: 150 start-page: 76 year: 1985 ident: 10.1016/S0006-291X(03)00109-8_BIB23 article-title: Measurement of protein using bicinchoninic acid publication-title: Anal. Biochem. doi: 10.1016/0003-2697(85)90442-7 – volume: 187 start-page: 265 year: 2001 ident: 10.1016/S0006-291X(03)00109-8_BIB12 article-title: Divergence and convergence of TGF-β/BMP signaling publication-title: J. Cell Physiol. doi: 10.1002/jcp.1080 – start-page: 993 issue: Suppl. 5 year: 1990 ident: 10.1016/S0006-291X(03)00109-8_BIB8 article-title: Pathogenesis of atherosclerosis: state of the art publication-title: Cardiovasc. Drugs Ther. doi: 10.1007/BF02018308 – volume: 85 start-page: 632 year: 1990 ident: 10.1016/S0006-291X(03)00109-8_BIB27 article-title: Oxygen-derived free radicals stimulate osteoclastic bone resorption in rodent bone in vitro and in vivo publication-title: J. Clin. Invest. doi: 10.1172/JCI114485 – volume: 17 start-page: 680 year: 1997 ident: 10.1016/S0006-291X(03)00109-8_BIB28 article-title: Lipid oxidation products have opposite effects on calcifying vascular cell and bone cell differentiation. A possible explanation for the paradox of arterial calcification in osteoporotic patients publication-title: Arterioscler. Thromb. Vasc. Biol. doi: 10.1161/01.ATV.17.4.680 – volume: 91 start-page: 1800 year: 1993 ident: 10.1016/S0006-291X(03)00109-8_BIB6 article-title: Bone morphogenetic protein expression in human atherosclerotic lesions publication-title: J. Clin. Invest. doi: 10.1172/JCI116391 – volume: 104 start-page: 249 year: 2001 ident: 10.1016/S0006-291X(03)00109-8_BIB18 article-title: Effects of lipid-lowering by simvastatin on human atherosclerotic lesions: a longitudinal study by high-resolution, noninvasive magnetic resonance imaging publication-title: Circulation doi: 10.1161/01.CIR.104.3.249 – volume: 157 start-page: 303 year: 2002 ident: 10.1016/S0006-291X(03)00109-8_BIB9 article-title: Cbfa1-independent decrease in osteoblast proliferation, osteopenia, and persistant embryonic eye vascularization in mice deficient in Lrp5, a Wnt coreceptor publication-title: J. Cell Biol. doi: 10.1083/jcb.200201089 – volume: 11 start-page: 2239 year: 2002 ident: 10.1016/S0006-291X(03)00109-8_BIB25 article-title: Obesity increases prostanoid-mediated vasosconstriction and vascular thromboxane receptor gene expression publication-title: J. Hypertens. doi: 10.1097/00004872-200211000-00024 – volume: 107 start-page: 513 year: 2001 ident: 10.1016/S0006-291X(03)00109-8_BIB10 article-title: LDL receptor-related protein 5 (LRP5) affects bone accrual and eye development publication-title: Cell doi: 10.1016/S0092-8674(01)00571-2 – volume: 340 start-page: 115 year: 1999 ident: 10.1016/S0006-291X(03)00109-8_BIB1 article-title: Atherosclerosis—an inflammatory disease publication-title: N. Engl. J. Med. doi: 10.1056/NEJM199901143400207 – volume: 130 start-page: 209 year: 2003 ident: 10.1016/S0006-291X(03)00109-8_BIB13 article-title: BMP signaling is required for septation of the outflow tract of the mammalian heart publication-title: Development doi: 10.1242/dev.00181 – volume: 100 start-page: 2824 year: 1997 ident: 10.1016/S0006-291X(03)00109-8_BIB17 article-title: Inhibition of rat vascular smooth muscle proliferation in vitro and in vivo by bone morphogenetic protein-2 publication-title: J. Clin. Invest. doi: 10.1172/JCI119830 – volume: 34 start-page: 987 year: 1999 ident: 10.1016/S0006-291X(03)00109-8_BIB29 article-title: State-of-the-Art lecture. Statins and blockers of the renin–angiotensin system: vascular protection beyond their primary mode of action publication-title: Hypertension doi: 10.1161/01.HYP.34.4.987 – volume: 8 start-page: 1257 year: 2002 ident: 10.1016/S0006-291X(03)00109-8_BIB4 article-title: Stabilization of atherosclerotic plaques: new mechanisms and clinical targets publication-title: Nat. Med. doi: 10.1038/nm1102-1257 – volume: 88 start-page: 3J year: 2001 ident: 10.1016/S0006-291X(03)00109-8_BIB2 article-title: What have we learned about the biology of atherosclerosis? The role of inflammation publication-title: Am. J. Cardiol. – volume: 150 start-page: 893 year: 1965 ident: 10.1016/S0006-291X(03)00109-8_BIB11 article-title: Bone: formation by autoinduction publication-title: Science doi: 10.1126/science.150.3698.893 – volume: 103 start-page: 2787 year: 1986 ident: 10.1016/S0006-291X(03)00109-8_BIB24 article-title: A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation publication-title: J. Cell Biol. doi: 10.1083/jcb.103.6.2787 – volume: 433 start-page: 559 year: 1998 ident: 10.1016/S0006-291X(03)00109-8_BIB5 article-title: Observations on bone formation and remodelling in advanced atherosclerotic lesions of human carotid arteries publication-title: Virchows Arch. doi: 10.1007/s004280050289 – volume: 88 start-page: 16E year: 2001 ident: 10.1016/S0006-291X(03)00109-8_BIB7 article-title: The development of calcium deposits in atherosclerotic lesions and their persistence after lipid regression publication-title: Am. J. Cardiol. doi: 10.1016/S0002-9149(01)01713-1 – volume: 21 start-page: 1998 year: 2001 ident: 10.1016/S0006-291X(03)00109-8_BIB15 article-title: Differential expression of bone matrix regulatory proteins in human atherosclerotic plaques publication-title: Arterioscler. Thromb. Vasc. Biol. doi: 10.1161/hq1201.100229 – volume: 286 start-page: 1946 year: 1999 ident: 10.1016/S0006-291X(03)00109-8_BIB20 article-title: Stimulation of bone formation in vitro and in rodents by statins publication-title: Science doi: 10.1126/science.286.5446.1946 – volume: 50 start-page: 127 year: 1983 ident: 10.1016/S0006-291X(03)00109-8_BIB3 article-title: Plaque rupture with severe pre-existing stenosis precipitating coronary thrombosis. Characteristics of coronary atherosclerotic plaques underlying fatal occlusive thrombi publication-title: Br. Heart J. doi: 10.1136/hrt.50.2.127 – volume: 39 start-page: 645 year: 2002 ident: 10.1016/S0006-291X(03)00109-8_BIB22 article-title: Native LDL induces proliferation of human vascular smooth muscle cells via redox-mediated activation of ERK 1/2 mitogen-activated protein kinases publication-title: Hypertension doi: 10.1161/hy0202.103473 – volume: 36 start-page: 120 year: 1999 ident: 10.1016/S0006-291X(03)00109-8_BIB14 article-title: BMP-2 gene expression and effects on human vascular smooth muscle cells publication-title: J. Vasc. Res. doi: 10.1159/000025634 |
| SSID | ssj0011469 |
| Score | 1.9213713 |
| Snippet | Bone morphogenetic proteins (BMPs) stimulate ectopic bone formation in skeletal muscle. Here we show that human vascular smooth muscle cells (VSMC) abundantly... |
| SourceID | proquest pubmed crossref elsevier |
| SourceType | Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 67 |
| SubjectTerms | Base Sequence BMP-statins Bone Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins - genetics Bone Morphogenetic Proteins - physiology Cell Division - physiology Cholesterol DNA Primers Gene Expression Regulation - drug effects HMG-CoA reductase inhibitor Humans Hydroxymethylglutaryl-CoA Reductase Inhibitors - pharmacology Lipoproteins, LDL - physiology Lovastatin - pharmacology Low-density lipoprotein Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - metabolism Plaque rupture Recombinant Proteins Reverse transcriptase polymerase chain reaction Risk factors RNA, Messenger - genetics Thymidine incorporation Transforming Growth Factor beta Transforming growth factor-β |
| Title | Lovastatin stimulates human vascular smooth muscle cell expression of bone morphogenetic protein-2, a potent inhibitor of low-density lipoprotein-stimulated cell growth |
| URI | https://dx.doi.org/10.1016/S0006-291X(03)00109-8 https://www.ncbi.nlm.nih.gov/pubmed/12593849 https://www.proquest.com/docview/19394336 https://www.proquest.com/docview/73044486 |
| Volume | 302 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELaqIgQXBC2PUig-IAQSbrO2kzjHZUW1vHqi0t6iOHZopI292s2q7YXfw89kxkl2xWFViVsSeexRZjKecb6ZIeRtWgptdKyYSXXBpE1HrFA2YZnJtODg0vEAxvxxkUwv5ddZPNsjkyEXBmGVve3vbHqw1v2Ts_5tni3qGnN8o4RnoxmoKf7fwYRfKVPU9dPfG5gHJt32LnDCcPQ2i6ebITx8H4kPYRKmdu1Pu_zPsA-dPyaPegeSjjsen5A96w7I4dhB8Nzc0nc0QDrDWfkBuf9puHowGRq7HZI_3xF_ir_gHYUPvMEGXnZFQ7c-OiBT6arxIETarFewDMXzfWpvetSso76i2jtLGw9y8qCEmAtJQ9GH2jH-kRZ0AdeupbW7qjWYjSXSzP01MwiZb2_pvF74gWDDhulW-rX01-3VU3J5_vnnZMr6hg2slLFqmTFYnErYqlKZKnRZJlanVgjNI26jrNQZBEBpoYWRtqxMolVawY0dcQuBFTfiGdl3wPwLQgs-KrIqrRJeafQxCg2eJ491lCY2NlofETmIKS_7aubYVGOeb2FrCNlD6eaRyIN0c3VETjdki66cx10EatCB_B-9zGHLuYv0zaAzOUgYX1_hrF-vcnCaMylEsnsE2F0JgTOMeN4p25ZbiFeFktnL_2fsmDwMkMSQmP-K7LfLtX0NrlWrT8K3c0Lujb98m178BQ99Iq8 |
| linkProvider | Elsevier |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9swDBa6DkN3GbZ2j27rysMwbMDUOpIf8rENVmRb2lML5CZYltwaiOUgcdD1st-znzlKthP0EBToTTZEmzApipQ_koR8TnKutIoE1YnKaGiSAc2EiWmqU8UZunTMgzHPL-LRVfhrEk22yLDPhXGwys72tzbdW-vuznH3NY9nZelyfIOYpYMJqqn7vyOekKdhxBNXQP_o7wrn4bJuOx84pm76Oo2nfYS_-TXg3_xTqNi0QW1yQP1GdPaSvOg8SDhpmXxFtozdJXsnFqPn6g6-gMd0-sPyXfLstB_tDPvObnvk39gBUN0_eAu4wivXwcsswLfrgx6aCouqRilCtVzga8Ad8IP508FmLdQFqNoaqGoUVI1a6JIhwVd9KC1l3yGDGY5tA6W9KRXajbmjmda3VDvMfHMH03JW9wQrNnT7put5fdvcvCZXZz8uhyPadWygeRiJhmrtqlNxUxQiFZnK89ioxHCuWMBMkOYqxQgoyRTXockLHSuRFHhhBsxgZMU0f0O2LTL_jkDGBllaJEXMCuWcjEyh68kiFSSxibRS-yTsxSTzrpy566oxlWvcmsPsOenKgEsvXSn2ydGKbNbW83iIQPQ6IO8ppsQ95yHSw15nJErYfb7Mmnq5kOg1pyHn8eYZaHhDjJxxxttW2dbcYsDKRZi-fzxjh2RndHk-luOfF78_kOcen-iz9D-S7Wa-NAfoZzXqk19H_wGGYyRB |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Lovastatin+stimulates+human+vascular+smooth+muscle+cell+expression+of+bone+morphogenetic+protein-2%2C+a+potent+inhibitor+of+low-density+lipoprotein-stimulated+cell+growth&rft.jtitle=Biochemical+and+biophysical+research+communications&rft.au=Emmanuele%2C+L&rft.au=Ortmann%2C+J&rft.au=Doerflinger%2C+T&rft.au=Traupe%2C+T&rft.date=2003-02-28&rft.issn=0006-291X&rft.volume=302&rft.issue=1&rft.spage=67&rft.epage=72&rft_id=info:doi/10.1016%2FS0006-291X%2803%2900109-8&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0006-291X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0006-291X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0006-291X&client=summon |