Influenza H7N9 Virus Neuraminidase-Specific Human Monoclonal Antibodies Inhibit Viral Egress and Protect from Lethal Influenza Infection in Mice

H7N9 avian influenza virus causes severe infections and might have the potential to trigger a major pandemic. Molecular determinants of human humoral immune response to N9 neuraminidase (NA) proteins, which exhibit unusual features compared with seasonal influenza virus NA proteins, are ill-defined....

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Published inCell host & microbe Vol. 26; no. 6; pp. 715 - 728.e8
Main Authors Gilchuk, Iuliia M., Bangaru, Sandhya, Gilchuk, Pavlo, Irving, Ryan P., Kose, Nurgun, Bombardi, Robin G., Thornburg, Natalie J., Creech, C. Buddy, Edwards, Kathryn M., Li, Sheng, Turner, Hannah L., Yu, Wenli, Zhu, Xueyong, Wilson, Ian A., Ward, Andrew B., Crowe, James E.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 11.12.2019
Subjects
Animals
antibodies
Antibodies, Heterophile - pharmacology
Antibodies, Monoclonal - pharmacology
Antibodies, Neutralizing - pharmacology
Antibodies, Viral - pharmacology
B lymphocyte
Birds
epitopes
Epitopes - immunology
H7N9
Humans
influenza A virus
Influenza A Virus, H7N9 Subtype - drug effects
Influenza A Virus, H7N9 Subtype - immunology
Influenza in Birds - prevention & control
Influenza in Birds - virology
Influenza Vaccines - immunology
Influenza, Human - prevention & control
Influenza, Human - virology
Mice
monoclonal
neuraminidase
Neuraminidase - immunology
Orthomyxoviridae Infections - prevention & control
Orthomyxoviridae Infections - virology
Pre-Exposure Prophylaxis
Vaccination
Vaccines, Inactivated
Viral Proteins - immunology
Virus Release - drug effects
epitopes
pre-exposure prophylaxis
neuraminidase
B lymphocyte
antibodies
monoclonal
H7N9
influenza A virus
Online AccessGet full text
ISSN1931-3128
1934-6069
1934-6069
DOI10.1016/j.chom.2019.10.003

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Abstract H7N9 avian influenza virus causes severe infections and might have the potential to trigger a major pandemic. Molecular determinants of human humoral immune response to N9 neuraminidase (NA) proteins, which exhibit unusual features compared with seasonal influenza virus NA proteins, are ill-defined. We isolated 35 human monoclonal antibodies (mAbs) from two H7N9 survivors and two vaccinees. These mAbs react to NA in a subtype-specific manner and recognize diverse antigenic sites on the surface of N9 NA, including epitopes overlapping with, or distinct from, the enzyme active site. Despite recognizing multiple antigenic sites, the mAbs use a common mechanism of action by blocking egress of nascent virions from infected cells, thereby providing an antiviral prophylactic and therapeutic protection in vivo in mice. Studies of breadth, potency, and diversity of antigenic recognition from four subjects suggest that vaccination with inactivated adjuvanted vaccine induce NA-reactive responses comparable to that of H7N9 natural infection. [Display omitted] •Human mAbs recognize several antigenic sites on influenza virus N9 NA•The mAbs act by blocking egress of nascent virions from infected cells•Human mAbs mediate prophylactic and therapeutic protection in vivo in mice•Protection is mediated by direct virus neutralization or Fc-region effector function Molecular determinants of the human B cell response to avian influenza N9 neuraminidase (NA) proteins, which differ from seasonal virus NAs, are ill-defined. Gilchuk et al. identify antibodies to multiple protective antigenic sites on N9 NA that block egress of nascent virions from infected cells and mediate protection in mice.
AbstractList H7N9 avian influenza virus causes severe infections and might have the potential to trigger a major pandemic. Molecular determinants of human humoral immune response to N9 neuraminidase (NA) proteins, which exhibit unusual features compared with seasonal influenza virus NA proteins, are ill-defined. We isolated 35 human monoclonal antibodies (mAbs) from two H7N9 survivors and two vaccinees. These mAbs react to NA in a subtype-specific manner and recognize diverse antigenic sites on the surface of N9 NA, including epitopes overlapping with, or distinct from, the enzyme active site. Despite recognizing multiple antigenic sites, the mAbs use a common mechanism of action by blocking egress of nascent virions from infected cells, thereby providing an antiviral prophylactic and therapeutic protection in vivo in mice. Studies of breadth, potency, and diversity of antigenic recognition from four subjects suggest that vaccination with inactivated adjuvanted vaccine induce NA-reactive responses comparable to that of H7N9 natural infection. Molecular determinants of the human B cell response to avian influenza N9 neuraminidase (NA) proteins, which differ from seasonal virus NAs, are ill-defined. Gilchuk et al. identify antibodies to multiple protective antigenic sites on N9 NA that block egress of nascent virions from infected cells and mediate protection in mice.
H7N9 avian influenza virus causes severe infections and might have the potential to trigger a major pandemic. Molecular determinants of human humoral immune response to N9 neuraminidase (NA) proteins, which exhibit unusual features compared with seasonal influenza virus NA proteins, are ill-defined. We isolated 35 human monoclonal antibodies (mAbs) from two H7N9 survivors and two vaccinees. These mAbs react to NA in a subtype-specific manner and recognize diverse antigenic sites on the surface of N9 NA, including epitopes overlapping with, or distinct from, the enzyme active site. Despite recognizing multiple antigenic sites, the mAbs use a common mechanism of action by blocking egress of nascent virions from infected cells, thereby providing an antiviral prophylactic and therapeutic protection in vivo in mice. Studies of breadth, potency, and diversity of antigenic recognition from four subjects suggest that vaccination with inactivated adjuvanted vaccine induce NA-reactive responses comparable to that of H7N9 natural infection.H7N9 avian influenza virus causes severe infections and might have the potential to trigger a major pandemic. Molecular determinants of human humoral immune response to N9 neuraminidase (NA) proteins, which exhibit unusual features compared with seasonal influenza virus NA proteins, are ill-defined. We isolated 35 human monoclonal antibodies (mAbs) from two H7N9 survivors and two vaccinees. These mAbs react to NA in a subtype-specific manner and recognize diverse antigenic sites on the surface of N9 NA, including epitopes overlapping with, or distinct from, the enzyme active site. Despite recognizing multiple antigenic sites, the mAbs use a common mechanism of action by blocking egress of nascent virions from infected cells, thereby providing an antiviral prophylactic and therapeutic protection in vivo in mice. Studies of breadth, potency, and diversity of antigenic recognition from four subjects suggest that vaccination with inactivated adjuvanted vaccine induce NA-reactive responses comparable to that of H7N9 natural infection.
H7N9 avian influenza virus causes severe infections and might have the potential to trigger a major pandemic. Molecular determinants of human humoral immune response to N9 neuraminidase (NA) proteins, which exhibit unusual features compared with seasonal influenza virus NA proteins, are ill-defined. We isolated 35 human monoclonal antibodies (mAbs) from two H7N9 survivors and two vaccinees. These mAbs react to NA in a subtype-specific manner and recognize diverse antigenic sites on the surface of N9 NA, including epitopes overlapping with, or distinct from, the enzyme active site. Despite recognizing multiple antigenic sites, the mAbs use a common mechanism of action by blocking egress of nascent virions from infected cells, thereby providing an antiviral prophylactic and therapeutic protection in vivo in mice. Studies of breadth, potency, and diversity of antigenic recognition from four subjects suggest that vaccination with inactivated adjuvanted vaccine induce NA-reactive responses comparable to that of H7N9 natural infection.
H7N9 avian influenza virus causes severe infections and might have the potential to trigger a major pandemic. Molecular determinants of human humoral immune response to N9 neuraminidase (NA) proteins, which exhibit unusual features compared with seasonal influenza virus NA proteins, are ill-defined. We isolated 35 human monoclonal antibodies (mAbs) from two H7N9 survivors and two vaccinees. These mAbs react to NA in a subtype-specific manner and recognize diverse antigenic sites on the surface of N9 NA, including epitopes overlapping with, or distinct from, the enzyme active site. Despite recognizing multiple antigenic sites, the mAbs use a common mechanism of action by blocking egress of nascent virions from infected cells, thereby providing an antiviral prophylactic and therapeutic protection in vivo in mice. Studies of breadth, potency, and diversity of antigenic recognition from four subjects suggest that vaccination with inactivated adjuvanted vaccine induce NA-reactive responses comparable to that of H7N9 natural infection. [Display omitted] •Human mAbs recognize several antigenic sites on influenza virus N9 NA•The mAbs act by blocking egress of nascent virions from infected cells•Human mAbs mediate prophylactic and therapeutic protection in vivo in mice•Protection is mediated by direct virus neutralization or Fc-region effector function Molecular determinants of the human B cell response to avian influenza N9 neuraminidase (NA) proteins, which differ from seasonal virus NAs, are ill-defined. Gilchuk et al. identify antibodies to multiple protective antigenic sites on N9 NA that block egress of nascent virions from infected cells and mediate protection in mice.
Author Bombardi, Robin G.
Wilson, Ian A.
Li, Sheng
Bangaru, Sandhya
Creech, C. Buddy
Turner, Hannah L.
Gilchuk, Iuliia M.
Edwards, Kathryn M.
Gilchuk, Pavlo
Irving, Ryan P.
Kose, Nurgun
Crowe, James E.
Zhu, Xueyong
Yu, Wenli
Ward, Andrew B.
Thornburg, Natalie J.
AuthorAffiliation 1 The Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA
5 Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
7 These authors contributed equally
3 Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA
8 Lead Contact
6 Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
4 Department of Medicine, School of Medicine, University of California, San Diego, San Diego, CA 92093, USA
2 Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
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Issue 6
Keywords epitopes
pre-exposure prophylaxis
neuraminidase
B lymphocyte
antibodies
monoclonal
H7N9
influenza A virus
Language English
License Copyright © 2019 Elsevier Inc. All rights reserved.
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AUTHOR CONTRIBUTIONS
X.Z. and W.Y. produced N9, N6, and N7 NA proteins. S.B., N.K., N.J.T., and I.M.G. performed initial screening and isolation of antibodies and isolated hybridomas. R.G.B. sequenced antibody clones and produced synthetic DNA in expression vectors. S.B. purified antibodies. S.B. and I.M.G. performed ELISA and ELLA NI; I.M.G. conducted egress assays and NA-Fluor NI assays and performed HAI. S.L. performed HDX-MS experiments. B.C. and K.M.E. directed the sample collection. H.L.T. and A.B.W. performed nsEM studies. I.M.G., P.G., and R.I. performed animal protection studies. S.B., I.M.G., and J.E.C. wrote the first draft of the manuscript. All authors revised and approved the final version of the manuscript.
OpenAccessLink http://www.cell.com/article/S193131281930527X/pdf
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PublicationDate 2019-12-11
PublicationDateYYYYMMDD 2019-12-11
PublicationDate_xml – month: 12
  year: 2019
  text: 2019-12-11
  day: 11
PublicationDecade 2010
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Cell host & microbe
PublicationTitleAlternate Cell Host Microbe
PublicationYear 2019
Publisher Elsevier Inc
Publisher_xml – name: Elsevier Inc
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Snippet H7N9 avian influenza virus causes severe infections and might have the potential to trigger a major pandemic. Molecular determinants of human humoral immune...
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SubjectTerms Animals
antibodies
Antibodies, Heterophile - pharmacology
Antibodies, Monoclonal - pharmacology
Antibodies, Neutralizing - pharmacology
Antibodies, Viral - pharmacology
B lymphocyte
Birds
epitopes
Epitopes - immunology
H7N9
Humans
influenza A virus
Influenza A Virus, H7N9 Subtype - drug effects
Influenza A Virus, H7N9 Subtype - immunology
Influenza in Birds - prevention & control
Influenza in Birds - virology
Influenza Vaccines - immunology
Influenza, Human - prevention & control
Influenza, Human - virology
Mice
monoclonal
neuraminidase
Neuraminidase - immunology
Orthomyxoviridae Infections - prevention & control
Orthomyxoviridae Infections - virology
Pre-Exposure Prophylaxis
Vaccination
Vaccines, Inactivated
Viral Proteins - immunology
Virus Release - drug effects
Title Influenza H7N9 Virus Neuraminidase-Specific Human Monoclonal Antibodies Inhibit Viral Egress and Protect from Lethal Influenza Infection in Mice
URI https://dx.doi.org/10.1016/j.chom.2019.10.003
https://www.ncbi.nlm.nih.gov/pubmed/31757769
https://www.proquest.com/docview/2317588035
https://pubmed.ncbi.nlm.nih.gov/PMC6941661
Volume 26
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