Induction of Cross-Reactive Antibodies by Immunization of Healthy Adults with Types Ia and Ib Group B Streptococcal Polysaccharide-Tetanus Toxoid Conjugate Vaccines

Types Ia and Ib group B streptococcal (GBS) capsular polysaccharides (PSs) are structural isomers but are antigenically distinct. Immunization of healthy adults with GBS type Ia PS-tetanus toxoid (Ia-TT) or Ib-TT glycoconjugate vaccines induced ⩾4-fold increases in specific immunoglobulin G to the h...

Full description

Saved in:
Bibliographic Details
Published inThe Journal of infectious diseases Vol. 185; no. 9; pp. 1277 - 1284
Main Authors Brigtsen, Anne Karin, Kasper, Dennis L., Baker, Carol J., Jennings, Harold J., Guttormsen, Hilde-Kari
Format Journal Article
LanguageEnglish
Published Chicago, IL The University of Chicago Press 01.05.2002
University of Chicago Press
Subjects
Online AccessGet full text
ISSN0022-1899
1537-6613
DOI10.1086/340324

Cover

More Information
Summary:Types Ia and Ib group B streptococcal (GBS) capsular polysaccharides (PSs) are structural isomers but are antigenically distinct. Immunization of healthy adults with GBS type Ia PS-tetanus toxoid (Ia-TT) or Ib-TT glycoconjugate vaccines induced ⩾4-fold increases in specific immunoglobulin G to the heterologous PS in more than two-thirds of subjects. Ib-TT vaccine-induced IgG bound with substantially higher affinity to homologous (Ib) than to heterologous (Ia) PS and promoted opsonophagocytic killing of GBS type Ib but not type Ia organisms. The failure of the Ib-TT- and Ia-TT-induced human antibodies to kill bacteria of the cross-reactive serotype contrasts with the results of previous studies in animals. Inhibition enzyme-linked immunosorbent assays demonstrated that Ib-TT-induced IgG to the homologous PS bound mainly to native Ib PS, whereas the cross-reactive antibodies recognized both native and derivative PSs. These results indicate that GBS Ia and Ib PSs should be included in a multivalent conjugate vaccine to prevent GBS disease.
Bibliography:ark:/67375/HXZ-L40SQ0X9-N
Present affiliation: Department of Pediatrics, Ullevål University Hospital, University of Oslo, Oslo, Norway.
istex:14FB9D3F02028F3FB6765194E688051AAC7E8D82
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0022-1899
1537-6613
DOI:10.1086/340324