Three-dimensional bright-field microscopy with isotropic resolution based on multi-view acquisition and image fusion reconstruction
Optical Projection Tomography (OPT) is a powerful three-dimensional imaging technique used for the observation of millimeter-scaled biological samples, compatible with bright-field and fluorescence contrast. OPT is affected by spatially variant artifacts caused by the fact that light diffraction is...
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Published in | Scientific reports Vol. 10; no. 1; p. 12771 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
29.07.2020
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
ISSN | 2045-2322 2045-2322 |
DOI | 10.1038/s41598-020-69730-4 |
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Abstract | Optical Projection Tomography (OPT) is a powerful three-dimensional imaging technique used for the observation of millimeter-scaled biological samples, compatible with bright-field and fluorescence contrast. OPT is affected by spatially variant artifacts caused by the fact that light diffraction is not taken into account by the straight-light propagation models used for reconstruction. These artifacts hinder high-resolution imaging with OPT. In this work we show that, by using a multiview imaging approach, a 3D reconstruction of the bright-field contrast can be obtained without the diffraction artifacts typical of OPT, drastically reducing the amount of acquired data, compared to previously reported approaches. The method, purely based on bright-field contrast of the unstained sample, provides a comprehensive picture of the sample anatomy, as demonstrated in vivo on
Arabidopsis thaliana
and zebrafish embryos. Furthermore, this bright-field reconstruction can be implemented on practically any multi-view light-sheet fluorescence microscope without complex hardware modifications or calibrations, complementing the fluorescence information with tissue anatomy. |
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AbstractList | Optical Projection Tomography (OPT) is a powerful three-dimensional imaging technique used for the observation of millimeter-scaled biological samples, compatible with bright-field and fluorescence contrast. OPT is affected by spatially variant artifacts caused by the fact that light diffraction is not taken into account by the straight-light propagation models used for reconstruction. These artifacts hinder high-resolution imaging with OPT. In this work we show that, by using a multiview imaging approach, a 3D reconstruction of the bright-field contrast can be obtained without the diffraction artifacts typical of OPT, drastically reducing the amount of acquired data, compared to previously reported approaches. The method, purely based on bright-field contrast of the unstained sample, provides a comprehensive picture of the sample anatomy, as demonstrated in vivo on Arabidopsis thaliana and zebrafish embryos. Furthermore, this bright-field reconstruction can be implemented on practically any multi-view light-sheet fluorescence microscope without complex hardware modifications or calibrations, complementing the fluorescence information with tissue anatomy.Optical Projection Tomography (OPT) is a powerful three-dimensional imaging technique used for the observation of millimeter-scaled biological samples, compatible with bright-field and fluorescence contrast. OPT is affected by spatially variant artifacts caused by the fact that light diffraction is not taken into account by the straight-light propagation models used for reconstruction. These artifacts hinder high-resolution imaging with OPT. In this work we show that, by using a multiview imaging approach, a 3D reconstruction of the bright-field contrast can be obtained without the diffraction artifacts typical of OPT, drastically reducing the amount of acquired data, compared to previously reported approaches. The method, purely based on bright-field contrast of the unstained sample, provides a comprehensive picture of the sample anatomy, as demonstrated in vivo on Arabidopsis thaliana and zebrafish embryos. Furthermore, this bright-field reconstruction can be implemented on practically any multi-view light-sheet fluorescence microscope without complex hardware modifications or calibrations, complementing the fluorescence information with tissue anatomy. Optical Projection Tomography (OPT) is a powerful three-dimensional imaging technique used for the observation of millimeter-scaled biological samples, compatible with bright-field and fluorescence contrast. OPT is affected by spatially variant artifacts caused by the fact that light diffraction is not taken into account by the straight-light propagation models used for reconstruction. These artifacts hinder high-resolution imaging with OPT. In this work we show that, by using a multiview imaging approach, a 3D reconstruction of the bright-field contrast can be obtained without the diffraction artifacts typical of OPT, drastically reducing the amount of acquired data, compared to previously reported approaches. The method, purely based on bright-field contrast of the unstained sample, provides a comprehensive picture of the sample anatomy, as demonstrated in vivo on Arabidopsis thaliana and zebrafish embryos. Furthermore, this bright-field reconstruction can be implemented on practically any multi-view light-sheet fluorescence microscope without complex hardware modifications or calibrations, complementing the fluorescence information with tissue anatomy. Optical Projection Tomography (OPT) is a powerful three-dimensional imaging technique used for the observation of millimeter-scaled biological samples, compatible with bright-field and fluorescence contrast. OPT is affected by spatially variant artifacts caused by the fact that light diffraction is not taken into account by the straight-light propagation models used for reconstruction. These artifacts hinder high-resolution imaging with OPT. In this work we show that, by using a multiview imaging approach, a 3D reconstruction of the bright-field contrast can be obtained without the diffraction artifacts typical of OPT, drastically reducing the amount of acquired data, compared to previously reported approaches. The method, purely based on bright-field contrast of the unstained sample, provides a comprehensive picture of the sample anatomy, as demonstrated in vivo on Arabidopsis thaliana and zebrafish embryos. Furthermore, this bright-field reconstruction can be implemented on practically any multi-view light-sheet fluorescence microscope without complex hardware modifications or calibrations, complementing the fluorescence information with tissue anatomy. |
ArticleNumber | 12771 |
Author | Pistocchi, Anna Magni, Vittorio Valentini, Gianluca Farina, Andrea D’Andrea, Cosimo Bassi, Andrea Calisesi, Gianmaria Candeo, Alessia Costa, Alex |
Author_xml | – sequence: 1 givenname: Gianmaria surname: Calisesi fullname: Calisesi, Gianmaria organization: Dipartimento di Fisica, Politecnico di Milano – sequence: 2 givenname: Alessia surname: Candeo fullname: Candeo, Alessia organization: Dipartimento di Fisica, Politecnico di Milano – sequence: 3 givenname: Andrea surname: Farina fullname: Farina, Andrea organization: Istituto di Fotonica e Nanotecnologie, Consiglio Nazionale delle ricerche – sequence: 4 givenname: Cosimo surname: D’Andrea fullname: D’Andrea, Cosimo organization: Dipartimento di Fisica, Politecnico di Milano – sequence: 5 givenname: Vittorio surname: Magni fullname: Magni, Vittorio organization: Dipartimento di Fisica, Politecnico di Milano – sequence: 6 givenname: Gianluca surname: Valentini fullname: Valentini, Gianluca organization: Dipartimento di Fisica, Politecnico di Milano, Istituto di Fotonica e Nanotecnologie, Consiglio Nazionale delle ricerche – sequence: 7 givenname: Anna surname: Pistocchi fullname: Pistocchi, Anna organization: Dipartimento di Biotecnologie Mediche e Medicina Traslazionale, Università degli Studi di Milano – sequence: 8 givenname: Alex surname: Costa fullname: Costa, Alex organization: Dipartimento di Bioscienze, Università degli Studi di Milano – sequence: 9 givenname: Andrea surname: Bassi fullname: Bassi, Andrea email: andrea1.bassi@polimi.it organization: Dipartimento di Fisica, Politecnico di Milano, Istituto di Fotonica e Nanotecnologie, Consiglio Nazionale delle ricerche |
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CitedBy_id | crossref_primary_10_1016_j_microc_2024_111458 crossref_primary_10_1073_pnas_2122937119 crossref_primary_10_1109_TBME_2022_3232068 crossref_primary_10_1021_acs_nanolett_3c01077 crossref_primary_10_1007_s12551_025_01301_1 crossref_primary_10_1364_BOE_423284 |
Cites_doi | 10.1371/journal.pone.0136213 10.1364/OL.39.000904 10.1038/nmeth985 10.1002/jbio.201500258 10.1364/OL.39.001053 10.1109/TMI.2011.2161590 10.1002/jemt.22981 10.1093/pcp/pcx045 10.1007/978-1-4939-9018-4_8 10.1364/OL.38.002654 10.1093/bioinformatics/btv387 10.1016/0030-4018(72)90243-X 10.1364/OL.385970 10.1038/s41377-018-0068-z 10.1088/0031-9155/50/19/015 10.1161/01.ATV.0000068685.72914.7E 10.1105/tpc.106.043042 10.1364/BOE.5.003367 10.1364/BOE.6.001253 10.1242/dev.116970 10.1364/JOSAA.29.000980 10.1364/OE.15.008029 10.1038/nmeth.1219 10.1002/jbio.201300162 10.1364/OE.21.025912 10.1371/journal.pone.0002853 10.1364/OE.24.024460 10.1364/OL.35.003363 10.1105/tpc.006437 10.1038/srep07325 10.1364/OPEX.13.004210 10.1371/journal.pone.0075646 10.1016/S0091-679X(04)76003-3 10.1117/1.JBO.21.5.056001 10.1016/j.ymeth.2017.10.009 10.1088/1361-6560/aa8945 10.1126/science.1068206 10.1126/science.1100035 10.1117/1.3640808 10.1073/pnas.0400417101 10.1364/OL.35.002732 10.1364/BOE.2.001340 10.1371/journal.pone.0050744 |
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References | Kumar, Chyou, Stein, Lu (CR6) 2012; 3 Andrews (CR12) 2016; 9 Soloviev, Zacharakis, Spiliopoulos, Favicchio, Correia, Arridge, Ripoll (CR18) 2012; 29 Candeo, Sana, Ferrari, D’Andrea, Maiuri, Valentini, Bassi (CR46) 2016; 21 Alanentalo (CR4) 2007; 4 Boot (CR5) 2008; 7 Lee (CR7) 2006; 18 Swoger, Verveer, Greger, Huisken, Stelzer (CR30) 2007; 15 Bryson-Richardson, Currie (CR2) 2004; 76 Bassi (CR34) 2010; 35 Mayer, Robert-Moreno, Sharpe, Swoger (CR21) 2018; 7 Fieramonti (CR16) 2015; 8 Costa, Candeo, Fieramonti, Valentini, Bassi (CR44) 2013; 8 Häusler (CR29) 1972; 6 Huisken, Swoger, Del Bene, Wittbrodt, Stelzer (CR31) 2004; 305 Arranz, Dong, Zhu, Rudin, Tsatsanis, Tian, Ripoll (CR8) 2013; 21 Zeng (CR15) 2014; 39 Candeo, Doccula, Valentini, Bassi, Costa (CR43) 2017; 58 Chen (CR10) 2015; 6 Ancora, Di Battista, Vidal, Avtzi, Zacharakis, Bassi (CR39) 2020; 45 Fieramonti (CR35) 2012; 7 Kak, Slaney (CR23) 1988 Arranz (CR36) 2014; 4 McGinty, Taylor, Chen, Bugeon, Lamb, Dallman, French (CR11) 2011; 2 Schmid, Huisken (CR47) 2015; 31 Fauver, Seibel, Richard Rahn, Meyer, Patten, Neumann, Nelson (CR26) 2005; 13 Chen (CR28) 2014; 5 Hyung-Taeg, Cosgrove (CR41) 2002; 14 Bassi, Fieramonti, D'Andrea, Valentini, Mione (CR37) 2011; 16 Correia (CR9) 2015; 10 Bassi, Schmid, Huisken (CR22) 2015; 142 Cheddad, Svensson, Sharpe, Georgsson, Ahlgren (CR17) 2012; 31 Armada, Doccula, Candeo, Valentini, Costa, Bassi (CR45) 2019; 10 Bassi, Fieramonti, D’Andrea, Mione, Valentini (CR13) 2011; 16 Cross, Cook, Lin, Chen, Rubinstein (CR42) 2003; 23 van der Horst, Kalkman (CR25) 2016; 24 Feng (CR14) 2013; 38 Miao (CR27) 2010; 35 Zagato, Toon, Smedt (CR32) 2018; 81 Nagai, Yamada, Tominaga, Ichikawa, Miyawaki (CR40) 2004; 101 Walls, Sled, Sharpe, Henkelman (CR38) 2005; 50 Walls, Coultas, Rossant, Henkelman (CR3) 2008; 3 Ancora (CR19) 2018; 136 Trull (CR24) 2017; 62 Mertz (CR33) 2019 Sharpe, Ahlgren, Perry, Hill, Ross, Hecksher-Sorensen, Baldock, Davidson (CR1) 2002; 296 Mayer, Robert-Moreno, Danuser, Stein, Sharpe, Swoger (CR20) 2014; 39 J McGinty (69730_CR11) 2011; 2 V Soloviev (69730_CR18) 2012; 29 JR Walls (69730_CR38) 2005; 50 Y Zeng (69730_CR15) 2014; 39 J Mayer (69730_CR20) 2014; 39 A Arranz (69730_CR36) 2014; 4 J Sharpe (69730_CR1) 2002; 296 G Feng (69730_CR14) 2013; 38 A Costa (69730_CR44) 2013; 8 D Ancora (69730_CR19) 2018; 136 L Fieramonti (69730_CR16) 2015; 8 AK Trull (69730_CR24) 2017; 62 A Bassi (69730_CR37) 2011; 16 R Bryson-Richardson (69730_CR2) 2004; 76 V Kumar (69730_CR6) 2012; 3 A Bassi (69730_CR34) 2010; 35 N Andrews (69730_CR12) 2016; 9 T Nagai (69730_CR40) 2004; 101 A Arranz (69730_CR8) 2013; 21 L Fieramonti (69730_CR35) 2012; 7 JR Walls (69730_CR3) 2008; 3 T Correia (69730_CR9) 2015; 10 J van der Horst (69730_CR25) 2016; 24 E Zagato (69730_CR32) 2018; 81 A Candeo (69730_CR43) 2017; 58 Q Miao (69730_CR27) 2010; 35 A Bassi (69730_CR22) 2015; 142 G Häusler (69730_CR29) 1972; 6 A Candeo (69730_CR46) 2016; 21 MJ Boot (69730_CR5) 2008; 7 NR Armada (69730_CR45) 2019; 10 LM Cross (69730_CR42) 2003; 23 B Schmid (69730_CR47) 2015; 31 J Mayer (69730_CR21) 2018; 7 AC Kak (69730_CR23) 1988 J Swoger (69730_CR30) 2007; 15 M Fauver (69730_CR26) 2005; 13 J Mertz (69730_CR33) 2019 L Chen (69730_CR10) 2015; 6 A Bassi (69730_CR13) 2011; 16 D Ancora (69730_CR39) 2020; 45 C Hyung-Taeg (69730_CR41) 2002; 14 T Alanentalo (69730_CR4) 2007; 4 K Lee (69730_CR7) 2006; 18 L Chen (69730_CR28) 2014; 5 A Cheddad (69730_CR17) 2012; 31 J Huisken (69730_CR31) 2004; 305 |
References_xml | – volume: 3 start-page: e2853 year: 2008 ident: CR3 article-title: Three-dimensional analysis of vascular development in the mouse embryo publication-title: PLoS ONE – volume: 6 start-page: 1253 year: 2015 end-page: 1261 ident: CR10 article-title: Mesoscopic in vivo 3-D tracking of sparse cell populations using angular multiplexed optical projection tomography publication-title: Biomed. Opt. Express – volume: 39 start-page: 904 issue: 4 year: 2014 end-page: 906 ident: CR15 article-title: Laser Doppler projection tomography publication-title: Opt. Lett. – volume: 305 start-page: 1007 year: 2004 end-page: 1009 ident: CR31 article-title: Optical sectioning deep inside live embryos by selective plane illumination microscopy publication-title: Science – volume: 39 start-page: 1053 issue: 4 year: 2014 end-page: 1056 ident: CR20 article-title: OPTiSPIM: integrating optical projection tomography in light sheet microscopy extends specimen characterization to non-fluorescent contrasts publication-title: Opt. Lett. – volume: 7 start-page: e50744 issue: 11 year: 2012 ident: CR35 article-title: Time-gated optical projection tomography allows visualization of adult zebrafish internal structures publication-title: PLoS ONE – volume: 45 start-page: 2191 issue: 8 year: 2020 end-page: 2194 ident: CR39 article-title: Hidden phase-retrieved fluorescence tomography publication-title: Opt. Lett. – volume: 35 start-page: 3363 year: 2010 end-page: 3365 ident: CR27 article-title: Resolution improvement in optical projection tomography by the focal scanning method publication-title: Opt. Lett. – volume: 6 start-page: 38 year: 1972 end-page: 42 ident: CR29 article-title: A method to increase the depth of focus by two-step image processing publication-title: Opt. Commun. – volume: 16 start-page: 100502 issue: 10 year: 2011 ident: CR37 article-title: In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography publication-title: J. Biomed. Opt. – volume: 5 start-page: 3367 issue: 10 year: 2014 end-page: 3375 ident: CR28 article-title: Remote focal scanning optical projection tomography with an electrically tunable lens publication-title: Biomed. Opt. Express – volume: 21 start-page: 25912 year: 2013 end-page: 25925 ident: CR8 article-title: Helical optical projection tomography publication-title: Opt. Express – volume: 15 start-page: 8029 year: 2007 end-page: 8042 ident: CR30 article-title: Multi-view image fusion improves resolution in three-dimensional microscopy publication-title: Opt. Express – volume: 4 start-page: 31 year: 2007 end-page: 33 ident: CR4 article-title: Tomographic molecular imaging and 3D quantification within adult mouse organs publication-title: Nat. Methods – volume: 4 start-page: 7325 year: 2014 ident: CR36 article-title: In-vivo optical tomography of small scattering specimens: time-lapse 3D imaging of the head eversion process in Drosophila melanogaster publication-title: Sci. Rep. – volume: 101 start-page: 10554 issue: 29 year: 2004 end-page: 10559 ident: CR40 article-title: Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins publication-title: Proc. Natl. Acad. Sci. – volume: 9 start-page: 414 issue: 4 year: 2016 end-page: 424 ident: CR12 article-title: Visualising apoptosis in live zebrafish using fluorescence lifetime imaging with optical projection tomography to map FRET biosensor activity in space and time publication-title: J. Biophotonics – volume: 81 start-page: 941 issue: 9 year: 2018 end-page: 958 ident: CR32 article-title: Technical implementations of light sheet microscopy publication-title: Microsc. Res. Technol. – volume: 7 start-page: 609 year: 2008 end-page: 612 ident: CR5 article-title: In vitro whole-organ imaging: 4D quantification of growing mouse limb buds publication-title: Nat. Methods – volume: 16 start-page: 100502 year: 2011 ident: CR13 article-title: In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography publication-title: J. Biomed. Opt. – volume: 10 start-page: 87 year: 2019 end-page: 101 ident: CR45 article-title: In vivo light sheet fluorescence microscopy of calcium oscillations in publication-title: Methods Mol. Biol. Calcium Signal. – volume: 2 start-page: 1340 issue: 5 year: 2011 end-page: 1350 ident: CR11 article-title: In vivo fluorescence lifetime optical projection tomography publication-title: Biomed. Opt. Express – volume: 18 start-page: 2145 year: 2006 end-page: 2156 ident: CR7 article-title: Visualizing plant development and gene expression in three dimensions using optical projection tomography publication-title: Plant Cell – volume: 8 start-page: e75646 year: 2013 ident: CR44 article-title: Calcium dynamics in root cells of visualized with selective plane illumination microscopy publication-title: PLoS ONE – volume: 31 start-page: 3398 issue: 20 year: 2015 end-page: 3400 ident: CR47 article-title: Real-time multi-view deconvolution publication-title: Bioinformatics – volume: 24 start-page: 24460 issue: 21 year: 2016 end-page: 24472 ident: CR25 article-title: Image resolution and deconvolution in optical tomography publication-title: Opt. Express – volume: 58 start-page: 1161 issue: 7 year: 2017 end-page: 1172 ident: CR43 article-title: Light sheet fluorescence microscopy quantifies calcium oscillations in root hairs of publication-title: Plant Cell Physiol – volume: 8 start-page: 52 issue: 1–2 year: 2015 end-page: 59 ident: CR16 article-title: Quantitative measurement of blood velocity in zebrafish with optical vector field tomography publication-title: J. Biophotonics – volume: 13 start-page: 4210 year: 2005 end-page: 4223 ident: CR26 article-title: Three-dimensional imaging of single isolated cell nuclei using optical projection tomography publication-title: Opt. Express – year: 2019 ident: CR33 publication-title: Introduction to Optical Microscopy – volume: 35 start-page: 2732 issue: 16 year: 2010 end-page: 2734 ident: CR34 article-title: Time-gated optical projection tomography publication-title: Opt. Lett. – volume: 50 start-page: 4645 issue: 19 year: 2005 end-page: 4665 ident: CR38 article-title: Correction of artefacts in optical projection tomography publication-title: Phys. Med. Biol. – volume: 62 start-page: 7784 issue: 19 year: 2017 ident: CR24 article-title: Point spread function based image reconstruction in optical projection tomography publication-title: Phys. Med. Biol. – volume: 10 start-page: e0136213 issue: 8 year: 2015 ident: CR9 article-title: Accelerated optical projection tomography applied to in vivo imaging of zebrafish publication-title: PLoS ONE – volume: 296 start-page: 541 year: 2002 end-page: 545 ident: CR1 article-title: Optical projection tomography as a tool for 3D microscopy and gene expression studies publication-title: Science – year: 1988 ident: CR23 publication-title: Principles of Computerized Tomographic Imaging – volume: 76 start-page: 37 year: 2004 end-page: 50 ident: CR2 article-title: Optical projection tomography for spatio-temporal analysis in the zebrafish publication-title: Methods Cell Biol. – volume: 14 start-page: 3237 issue: 12 year: 2002 end-page: 3253 ident: CR41 article-title: Regulation of root hair initiation and expansin gene expression in Arabidopsis publication-title: Plant Cell – volume: 23 start-page: 911 year: 2003 end-page: 912 ident: CR42 article-title: Rapid analysis of angiogenesis drugs in a live fluorescent zebrafish assay publication-title: Arterioscler. Thromb. Vasc. Biol. – volume: 7 start-page: 70 year: 2018 ident: CR21 article-title: Attenuation artifacts in light sheet fluorescence microscopy corrected by OPTiSPIM publication-title: Light Sci. Appl. – volume: 3 start-page: 282 year: 2012 ident: CR6 article-title: Optical projection tomography reveals dynamics of HEV growth after immunization with protein plus CFA and features shared with HEVs in acute autoinflammatory lymphadenopathy publication-title: Front. Immunol. – volume: 142 start-page: 1016 year: 2015 end-page: 1020 ident: CR22 article-title: Optical tomography complements light sheet microscopy for in toto imaging of zebrafish development publication-title: Development – volume: 38 start-page: 2654 issue: 15 year: 2013 end-page: 2656 ident: CR14 article-title: Laser speckle projection tomography publication-title: Opt. Lett. – volume: 136 start-page: 81 year: 2018 end-page: 89 ident: CR19 article-title: Optical projection tomography via phase retrieval algorithms publication-title: Methods – volume: 21 start-page: 056001 issue: 5 year: 2016 ident: CR46 article-title: Virtual unfolding of light sheet fluorescence microscopy dataset for quantitative analysis of the mouse intestine publication-title: J. Biomed. Opt. – volume: 31 start-page: 1 issue: 1 year: 2012 end-page: 15 ident: CR17 article-title: Image processing assisted algorithms for optical projection tomography publication-title: IEEE Trans. Med. Imaging – volume: 29 start-page: 980 year: 2012 end-page: 988 ident: CR18 article-title: Tomographic imaging with polarized light publication-title: J. Opt. Soc. Am. A – volume: 10 start-page: e0136213 issue: 8 year: 2015 ident: 69730_CR9 publication-title: PLoS ONE doi: 10.1371/journal.pone.0136213 – volume: 39 start-page: 904 issue: 4 year: 2014 ident: 69730_CR15 publication-title: Opt. Lett. doi: 10.1364/OL.39.000904 – volume: 4 start-page: 31 year: 2007 ident: 69730_CR4 publication-title: Nat. Methods doi: 10.1038/nmeth985 – volume: 9 start-page: 414 issue: 4 year: 2016 ident: 69730_CR12 publication-title: J. Biophotonics doi: 10.1002/jbio.201500258 – volume: 39 start-page: 1053 issue: 4 year: 2014 ident: 69730_CR20 publication-title: Opt. Lett. doi: 10.1364/OL.39.001053 – volume: 3 start-page: 282 year: 2012 ident: 69730_CR6 publication-title: Front. Immunol. – volume: 31 start-page: 1 issue: 1 year: 2012 ident: 69730_CR17 publication-title: IEEE Trans. Med. Imaging doi: 10.1109/TMI.2011.2161590 – volume: 81 start-page: 941 issue: 9 year: 2018 ident: 69730_CR32 publication-title: Microsc. Res. Technol. doi: 10.1002/jemt.22981 – volume: 58 start-page: 1161 issue: 7 year: 2017 ident: 69730_CR43 publication-title: Plant Cell Physiol doi: 10.1093/pcp/pcx045 – volume: 10 start-page: 87 year: 2019 ident: 69730_CR45 publication-title: Methods Mol. Biol. Calcium Signal. doi: 10.1007/978-1-4939-9018-4_8 – volume: 38 start-page: 2654 issue: 15 year: 2013 ident: 69730_CR14 publication-title: Opt. Lett. doi: 10.1364/OL.38.002654 – volume: 31 start-page: 3398 issue: 20 year: 2015 ident: 69730_CR47 publication-title: Bioinformatics doi: 10.1093/bioinformatics/btv387 – volume: 6 start-page: 38 year: 1972 ident: 69730_CR29 publication-title: Opt. Commun. doi: 10.1016/0030-4018(72)90243-X – volume-title: Introduction to Optical Microscopy year: 2019 ident: 69730_CR33 – volume: 45 start-page: 2191 issue: 8 year: 2020 ident: 69730_CR39 publication-title: Opt. Lett. doi: 10.1364/OL.385970 – volume: 7 start-page: 70 year: 2018 ident: 69730_CR21 publication-title: Light Sci. Appl. doi: 10.1038/s41377-018-0068-z – volume-title: Principles of Computerized Tomographic Imaging year: 1988 ident: 69730_CR23 – volume: 50 start-page: 4645 issue: 19 year: 2005 ident: 69730_CR38 publication-title: Phys. Med. Biol. doi: 10.1088/0031-9155/50/19/015 – volume: 23 start-page: 911 year: 2003 ident: 69730_CR42 publication-title: Arterioscler. Thromb. Vasc. Biol. doi: 10.1161/01.ATV.0000068685.72914.7E – volume: 18 start-page: 2145 year: 2006 ident: 69730_CR7 publication-title: Plant Cell doi: 10.1105/tpc.106.043042 – volume: 5 start-page: 3367 issue: 10 year: 2014 ident: 69730_CR28 publication-title: Biomed. Opt. Express doi: 10.1364/BOE.5.003367 – volume: 6 start-page: 1253 year: 2015 ident: 69730_CR10 publication-title: Biomed. Opt. Express doi: 10.1364/BOE.6.001253 – volume: 142 start-page: 1016 year: 2015 ident: 69730_CR22 publication-title: Development doi: 10.1242/dev.116970 – volume: 29 start-page: 980 year: 2012 ident: 69730_CR18 publication-title: J. Opt. Soc. Am. A doi: 10.1364/JOSAA.29.000980 – volume: 15 start-page: 8029 year: 2007 ident: 69730_CR30 publication-title: Opt. Express doi: 10.1364/OE.15.008029 – volume: 7 start-page: 609 year: 2008 ident: 69730_CR5 publication-title: Nat. Methods doi: 10.1038/nmeth.1219 – volume: 8 start-page: 52 issue: 1–2 year: 2015 ident: 69730_CR16 publication-title: J. Biophotonics doi: 10.1002/jbio.201300162 – volume: 21 start-page: 25912 year: 2013 ident: 69730_CR8 publication-title: Opt. Express doi: 10.1364/OE.21.025912 – volume: 3 start-page: e2853 year: 2008 ident: 69730_CR3 publication-title: PLoS ONE doi: 10.1371/journal.pone.0002853 – volume: 24 start-page: 24460 issue: 21 year: 2016 ident: 69730_CR25 publication-title: Opt. Express doi: 10.1364/OE.24.024460 – volume: 35 start-page: 3363 year: 2010 ident: 69730_CR27 publication-title: Opt. Lett. doi: 10.1364/OL.35.003363 – volume: 14 start-page: 3237 issue: 12 year: 2002 ident: 69730_CR41 publication-title: Plant Cell doi: 10.1105/tpc.006437 – volume: 4 start-page: 7325 year: 2014 ident: 69730_CR36 publication-title: Sci. Rep. doi: 10.1038/srep07325 – volume: 13 start-page: 4210 year: 2005 ident: 69730_CR26 publication-title: Opt. Express doi: 10.1364/OPEX.13.004210 – volume: 8 start-page: e75646 year: 2013 ident: 69730_CR44 publication-title: PLoS ONE doi: 10.1371/journal.pone.0075646 – volume: 76 start-page: 37 year: 2004 ident: 69730_CR2 publication-title: Methods Cell Biol. doi: 10.1016/S0091-679X(04)76003-3 – volume: 21 start-page: 056001 issue: 5 year: 2016 ident: 69730_CR46 publication-title: J. Biomed. Opt. doi: 10.1117/1.JBO.21.5.056001 – volume: 136 start-page: 81 year: 2018 ident: 69730_CR19 publication-title: Methods doi: 10.1016/j.ymeth.2017.10.009 – volume: 62 start-page: 7784 issue: 19 year: 2017 ident: 69730_CR24 publication-title: Phys. Med. Biol. doi: 10.1088/1361-6560/aa8945 – volume: 296 start-page: 541 year: 2002 ident: 69730_CR1 publication-title: Science doi: 10.1126/science.1068206 – volume: 305 start-page: 1007 year: 2004 ident: 69730_CR31 publication-title: Science doi: 10.1126/science.1100035 – volume: 16 start-page: 100502 issue: 10 year: 2011 ident: 69730_CR37 publication-title: J. Biomed. Opt. doi: 10.1117/1.3640808 – volume: 101 start-page: 10554 issue: 29 year: 2004 ident: 69730_CR40 publication-title: Proc. Natl. Acad. Sci. doi: 10.1073/pnas.0400417101 – volume: 16 start-page: 100502 year: 2011 ident: 69730_CR13 publication-title: J. Biomed. Opt. doi: 10.1117/1.3640808 – volume: 35 start-page: 2732 issue: 16 year: 2010 ident: 69730_CR34 publication-title: Opt. Lett. doi: 10.1364/OL.35.002732 – volume: 2 start-page: 1340 issue: 5 year: 2011 ident: 69730_CR11 publication-title: Biomed. Opt. Express doi: 10.1364/BOE.2.001340 – volume: 7 start-page: e50744 issue: 11 year: 2012 ident: 69730_CR35 publication-title: PLoS ONE doi: 10.1371/journal.pone.0050744 |
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