Multi-residue determination of phenolic and salicylanilide anthelmintics and related compounds in bovine kidney by liquid chromatography–tandem mass spectrometry

• Published in: Journal of Chromatography A Vol. 1216; no. 46; pp. 8200 - 8205
• Main Authors: Caldow, M., Sharman, M., Kelly, M., Day, J., Hird, S., Tarbin, J.A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 13.11.2009; Amsterdam; New York: Elsevier
• Subjects: analysis; Animals; Anthelmintics; Anthelmintics - analysis; Cattle; chemistry; Chromatography, Liquid; Chromatography, Liquid - methods; Closantel; drug residues; Drug Residues - analysis; Kidney; Kidney - chemistry; kidneys; LC–MS/MS; liquid chromatography; mass spectrometry; Method validation; methods; multiresidue analysis; Nitroxinil; Oxyclozanide; phenolic compounds; Phenols; Phenols - analysis; Rafoxanide; salicyanalides; Salicylanilides; Salicylanilides - analysis; solid phase extraction; Tandem Mass Spectrometry; Tandem Mass Spectrometry - methods; Rafoxanide; Closantel; Method validation; Nitroxinil; LC–MS/MS; Oxyclozanide
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2009.04.008

This paper describes an analytical method for four phenolic and salicylanilide anthelmintics authorised for use within the EU (nitroxinil, oxyclozanide, rafoxanide and closantel) in bovine kidney, and the extension of this procedure to include a number of related compounds; ioxynil, niclosamide, salicylanide and 3-trifluoromethyl-4-nitrophenol (TFM). The method comprises a solvent extraction with 1% acetic acid in acetone and clean-up using a mixed-mode anion-exchange solid phase extraction column. Determination is by reversed phase LC–MS/MS. The method was validated to the latest EU requirements (Commission Decision 2002/657/EC) using both spiked and incurred tissues and was subject to second laboratory evaluation.