Generation of ‘designer erythroblasts’ lacking one or more blood group systems from CRISPR/Cas9 gene‐edited human‐induced pluripotent stem cells - Pandey, Priyanka; Zhang, Nanyan; Curtis, Brian R.; Newman, Peter J.; Denomme, Gregory A. (Journal of cellular and molecular medicine, 2021)
Keywords: Alloantibodies; alloimmunization; Antibodies; antibody identification; Antigens; Biomarkers; Blood & organ donations; Blood Group Antigens - genetics; Blood Group Antigens - metabolism; blood group systems; Blood groups; CD34 antigen; Cell Differentiation; Cell Line; Cell Lineage; Chromosomes; Cloning; CRISPR; CRISPR-Cas Systems; Custom design; Erythroblasts; Erythroblasts - cytology; Erythroblasts - metabolism; Erythrocytes; Gene Editing; Gene Knockdown Techniques; Genes; Genome editing; Genotyping; Glycoproteins; Hematopoiesis; Hematopoietic stem cells; hiPSC; Histocytochemistry; Humans; Immunophenotyping; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Isoimmunization; Laboratories; Original; Phenotypes; Pluripotency; Progenitor cells; Proteins; Puromycin; RNA, Guide, CRISPR-Cas Systems - genetics; Stem cells; Canada; United States--US; Switzerland; CRISPR; antibody identification; blood group systems; alloimmunization; hiPSC; erythroblasts