Metabolic fingerprinting of high-fat plasma samples processed by centrifugation- and filtration-based protein precipitation delineates significant differences in metabolite information coverage - Barri, Thaer; Holmer-Jensen, Jens; Hermansen, Kjeld; Dragsted, Lars O. (Analytica chimica acta, 2012)
Keywords: amino acids; Analytical chemistry; blood proteins; Blood Proteins - isolation & purification; blood serum; Centrifugation; Centrifugation - methods; Chemical Precipitation; Chemistry; Exact sciences and technology; fasting; Fasting - blood; Fats - metabolism; Female; Filtration; Filtration - methods; Humans; lipid content; Male; Metabolic fingerprinting; metabolites; Metabolomics; Metabolomics - methods; molecular weight; nutritional intervention; Plasma - metabolism; Plasma protein precipitation; Postprandial Period; QTOF/MS; Plasma protein precipitation; Metabolomics; QTOF/MS; Filtration; Metabolic fingerprinting; Centrifugation; Metabolite; Internal standard; Laboratory; Multivariate analysis; Modeling; Blood plasma; Lipophilic compound; Improvement; Precipitation; Endogenous; Serum; Molecular mass; Quantitative analysis; Coupled method; Sample; Use; Error; Matrix effect; Marker; Protein; Aminoacid; Fingerprint method; Fat; Principal component analysis