Retinyl Ester Storage Particles (Retinosomes) from the Retinal Pigmented Epithelium Resemble Lipid Droplets in Other Tissues

• Published in: The Journal of biological chemistry Vol. 286; no. 19; pp. 17248 - 17258
• Main Authors: Orban, Tivadar, Palczewska, Grazyna, Palczewski, Krzysztof
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 13.05.2011; American Society for Biochemistry and Molecular Biology
• Subjects: Acyltransferases - chemistry; Animals; Cattle; Chromatography, Thin Layer - methods; Esters - chemistry; Lipid Droplet; Lipid-binding Protein; Lipids; Lipids - chemistry; Lipoproteins - chemistry; Mice; NIH 3T3 Cells; Proteomics - methods; Retina - metabolism; Retinal Metabolism; Retinal Pigment Epithelium - metabolism; Retinoid; Retinol-Binding Proteins - chemistry; Retinyl Esters; Subcellular Fractions - metabolism; Vitamin A; Vitamin A - chemistry; Lipid Droplet; Lipid-binding Protein; Vitamin A; Retinal Metabolism; Retinoid
• ISSN: 0021-9258; 1083-351X; 1067-8816; 1083-351X
• DOI: 10.1074/jbc.M110.195198

Levels of many hydrophobic cellular substances are tightly regulated because of their potential cytotoxicity. These compounds tend to self-aggregate in cytoplasmic storage depots termed lipid droplets/bodies that have well defined structures that contain additional components, including cholesterol and various proteins. Hydrophobic substances in these structures become mobilized in a specific and regulated manner as dictated by cellular requirements. Retinal pigmented epithelial cells in the eye produce retinyl ester-containing lipid droplets named retinosomes. These esters are mobilized to replenish the visual chromophore, 11-cis-retinal, and their storage ensures proper visual function despite fluctuations in dietary vitamin A intake. But it remains unclear whether retinosomes are structures specific to the eye or similar to lipid droplets in other organs/tissues that contain substances other than retinyl esters. Thus, we initially investigated the production of these lipid droplets in experimental cell lines expressing lecithin:retinol acyltransferase, a key enzyme involved in formation of retinyl ester-containing retinosomes from all-trans-retinol. We found that retinosomes and oleate-derived lipid droplets form and co-localize concomitantly, indicating their intrinsic structural similarities. Next, we isolated native retinosomes from bovine retinal pigmented epithelium and found that their protein and hydrophobic small molecular constituents were similar to those of lipid droplets reported for other experimental cell lines and tissues. These unexpected findings suggest a common mechanism for lipid droplet formation that exhibits broad chemical specificity for the hydrophobic substances being stored.