Pretreatment with Baicalin Attenuates Hypoxia and Glucose Deprivation-Induced Injury in SH-SY5Y Cells

• Published in: Chinese journal of integrative medicine Vol. 22; no. 3; pp. 201 - 206
• Main Author: 周庆博 鞠小宁 王晓云 王美红 孔峰 孙超 毕建忠
• Format: Journal Article
• Language: English
• Published: Beijing Chinese Association of Traditional and Western Medicine 01.03.2016
• Subjects: Apoptosis - drug effects; Caspase 3 - genetics; Caspase 3 - metabolism; Cell Death - drug effects; Cell Hypoxia - drug effects; Cell Line, Tumor; Cell Survival - drug effects; Flavonoids - pharmacology; Glucose - metabolism; Humans; Medicine; Medicine & Public Health; Nerve Tissue Proteins - metabolism; NF-kappa B - metabolism; Original Article; Real-Time Polymerase Chain Reaction; Receptors, N-Methyl-D-Aspartate - metabolism; Reperfusion; RNA, Messenger - genetics; RNA, Messenger - metabolism; 临床; 医学; 结合医学; 诊断; N-methyl-d-aspartic acid receptor-1; B; baicalin; neuroprotection; hypoxia and glucose deptivation-reperfusion; apoptosis; caspase-3; nuclear factor; nuclear factor-κB
• ISSN: 1672-0415; 1993-0402; 1993-0402
• DOI: 10.1007/s11655-015-2326-8

Objective： To explore the neuroprotective effects of baicalin against hypoxia and glucose deprivation- reperfusion （OGD/RO）-induced injury in SH-SY5Y cells. Methods： SH-SY5Y cells were divided into a control group, a OGD/RO group, which was subject to OGD/RO induction; and 3 baicalin groups subject to baicalin （1, 5, 25 μmol/L） for 2 h before induction of OGD/RO （low-, medium-, and high-dose baicalin groups）. Cell viability was detected by thiazolyl blue tetrazoliurn bromide （MTT） assay and flow cytometric analysis was used to detect cell apoptosis. Real-time polymerase chain reaction was performed to determine the mRNA expression of caspase-3 gene. Westem blot analysis was conducted to determine the expression of nuclear factor （NF）- κB and N-methyl-d- aspartic acid receptor-1 （NMDAR1）. Results： Baicalin could significantly attenuate OGD/RO mediated apoptotic cell death in SH-SY5Y cells; the apoptosis rates in the low-, medium- and high-dose groups were 12.1%, 7.9%, and 5.4%, respectively. Western blot and real-time PCR analysis revealed that significant decrease in caspase-3 expression in the baicalin group compared with the OGD/RO group （P〈0.01）. Additionally, down-regulation of NF-K B and NMDAR1 was observed in the baicalin group compared with those obtained from the OGD/RO group. Compared with the low-dose baicalin group, remarkable decrease was noted in the medium- and high-dose groups （P〈0.01）. Conclusion： Baicalin pre-treatment attenuates brain ischemia reperfusion injury by suppressing cellular apoptosis.