Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization

Interest in extracellular vesicles and in particular microvesicles and exosomes, which are constitutively produced by cells, is on the rise for their huge potential as biomarkers in a high number of disorders and pathologies as they are considered as carriers of information among cells, as well as b...

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Published inSensors (Basel, Switzerland) Vol. 18; no. 10; p. 3175
Main Authors Chiriacò, Maria Serena, Bianco, Monica, Nigro, Annamaria, Primiceri, Elisabetta, Ferrara, Francesco, Romano, Alessandro, Quattrini, Angelo, Furlan, Roberto, Arima, Valentina, Maruccio, Giuseppe
Format Journal Article
LanguageEnglish
Published Switzerland MDPI 20.09.2018
MDPI AG
Subjects
Animals
Biomarkers - analysis
biosensors
Body Fluids - cytology
Cell-Derived Microparticles - chemistry
Cell-Derived Microparticles - metabolism
exosomes
Exosomes - chemistry
Exosomes - metabolism
extracellular vesicles
Humans
Lab-On-A-Chip Devices
lab-on-chip
microfabrication
microfluidics
Review
microfabrication
lab-on-chip
biosensors
extracellular vesicles
microfluidics
exosomes
Online AccessGet full text
ISSN1424-8220
1424-8220
DOI10.3390/s18103175

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Abstract Interest in extracellular vesicles and in particular microvesicles and exosomes, which are constitutively produced by cells, is on the rise for their huge potential as biomarkers in a high number of disorders and pathologies as they are considered as carriers of information among cells, as well as being responsible for the spreading of diseases. Current methods of analysis of microvesicles and exosomes do not fulfill the requirements for their in-depth investigation and the complete exploitation of their diagnostic and prognostic value. Lab-on-chip methods have the potential and capabilities to bridge this gap and the technology is mature enough to provide all the necessary steps for a completely automated analysis of extracellular vesicles in body fluids. In this paper we provide an overview of the biological role of extracellular vesicles, standard biochemical methods of analysis and their limits, and a survey of lab-on-chip methods that are able to meet the needs of a deeper exploitation of these biological entities to drive their use in common clinical practice.
AbstractList Interest in extracellular vesicles and in particular microvesicles and exosomes, which are constitutively produced by cells, is on the rise for their huge potential as biomarkers in a high number of disorders and pathologies as they are considered as carriers of information among cells, as well as being responsible for the spreading of diseases. Current methods of analysis of microvesicles and exosomes do not fulfill the requirements for their in-depth investigation and the complete exploitation of their diagnostic and prognostic value. Lab-on-chip methods have the potential and capabilities to bridge this gap and the technology is mature enough to provide all the necessary steps for a completely automated analysis of extracellular vesicles in body fluids. In this paper we provide an overview of the biological role of extracellular vesicles, standard biochemical methods of analysis and their limits, and a survey of lab-on-chip methods that are able to meet the needs of a deeper exploitation of these biological entities to drive their use in common clinical practice.Interest in extracellular vesicles and in particular microvesicles and exosomes, which are constitutively produced by cells, is on the rise for their huge potential as biomarkers in a high number of disorders and pathologies as they are considered as carriers of information among cells, as well as being responsible for the spreading of diseases. Current methods of analysis of microvesicles and exosomes do not fulfill the requirements for their in-depth investigation and the complete exploitation of their diagnostic and prognostic value. Lab-on-chip methods have the potential and capabilities to bridge this gap and the technology is mature enough to provide all the necessary steps for a completely automated analysis of extracellular vesicles in body fluids. In this paper we provide an overview of the biological role of extracellular vesicles, standard biochemical methods of analysis and their limits, and a survey of lab-on-chip methods that are able to meet the needs of a deeper exploitation of these biological entities to drive their use in common clinical practice.
Interest in extracellular vesicles and in particular microvesicles and exosomes, which are constitutively produced by cells, is on the rise for their huge potential as biomarkers in a high number of disorders and pathologies as they are considered as carriers of information among cells, as well as being responsible for the spreading of diseases. Current methods of analysis of microvesicles and exosomes do not fulfill the requirements for their in-depth investigation and the complete exploitation of their diagnostic and prognostic value. Lab-on-chip methods have the potential and capabilities to bridge this gap and the technology is mature enough to provide all the necessary steps for a completely automated analysis of extracellular vesicles in body fluids. In this paper we provide an overview of the biological role of extracellular vesicles, standard biochemical methods of analysis and their limits, and a survey of lab-on-chip methods that are able to meet the needs of a deeper exploitation of these biological entities to drive their use in common clinical practice.
Author Furlan, Roberto
Nigro, Annamaria
Arima, Valentina
Quattrini, Angelo
Maruccio, Giuseppe
Romano, Alessandro
Bianco, Monica
Chiriacò, Maria Serena
Primiceri, Elisabetta
Ferrara, Francesco
AuthorAffiliation 4 Department of Mathematics and Physics, University of Salento, via Monteroni, 73100 Lecce, Italy
3 STMicroelectronics, Via Monteroni, I-73100 Lecce, Italy
1 CNR NANOTEC Institute of Nanotechnology, via Monteroni, 73100 Lecce, Italy; monica.bianco@nanotec.cnr.it (M.B.); elisabetta.primiceri@nanotec.cnr.it (E.P.); francesco.ferrara@st.com (F.F.); valentina.arima@nanotec.cnr.it (V.A.); giuseppe.maruccio@unisalento.it (G.M.)
2 Institute of Experimental Neurology, Division of Neuroscience, San Raffaele Scientific Institute, 20132 Milan, Italy; nigro.annamaria@hsr.it (A.N.); romano.alessandro@hsr.it (A.R.); quattrini.angelo@hsr.it (A.Q.); furlan.roberto@hsr.it (R.F.)
AuthorAffiliation_xml – name: 1 CNR NANOTEC Institute of Nanotechnology, via Monteroni, 73100 Lecce, Italy; monica.bianco@nanotec.cnr.it (M.B.); elisabetta.primiceri@nanotec.cnr.it (E.P.); francesco.ferrara@st.com (F.F.); valentina.arima@nanotec.cnr.it (V.A.); giuseppe.maruccio@unisalento.it (G.M.)
– name: 2 Institute of Experimental Neurology, Division of Neuroscience, San Raffaele Scientific Institute, 20132 Milan, Italy; nigro.annamaria@hsr.it (A.N.); romano.alessandro@hsr.it (A.R.); quattrini.angelo@hsr.it (A.Q.); furlan.roberto@hsr.it (R.F.)
– name: 3 STMicroelectronics, Via Monteroni, I-73100 Lecce, Italy
– name: 4 Department of Mathematics and Physics, University of Salento, via Monteroni, 73100 Lecce, Italy
Author_xml – sequence: 1
  givenname: Maria Serena
  orcidid: 0000-0002-7195-267X
  surname: Chiriacò
  fullname: Chiriacò, Maria Serena
– sequence: 2
  givenname: Monica
  orcidid: 0000-0002-1791-7232
  surname: Bianco
  fullname: Bianco, Monica
– sequence: 3
  givenname: Annamaria
  orcidid: 0000-0001-9570-7276
  surname: Nigro
  fullname: Nigro, Annamaria
– sequence: 4
  givenname: Elisabetta
  surname: Primiceri
  fullname: Primiceri, Elisabetta
– sequence: 5
  givenname: Francesco
  orcidid: 0000-0002-7290-9542
  surname: Ferrara
  fullname: Ferrara, Francesco
– sequence: 6
  givenname: Alessandro
  surname: Romano
  fullname: Romano, Alessandro
– sequence: 7
  givenname: Angelo
  orcidid: 0000-0002-8226-8320
  surname: Quattrini
  fullname: Quattrini, Angelo
– sequence: 8
  givenname: Roberto
  orcidid: 0000-0001-7376-9425
  surname: Furlan
  fullname: Furlan, Roberto
– sequence: 9
  givenname: Valentina
  orcidid: 0000-0002-3429-8365
  surname: Arima
  fullname: Arima, Valentina
– sequence: 10
  givenname: Giuseppe
  orcidid: 0000-0001-7669-0253
  surname: Maruccio
  fullname: Maruccio, Giuseppe
BackLink https://www.ncbi.nlm.nih.gov/pubmed/30241303$$D View this record in MEDLINE/PubMed
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Snippet Interest in extracellular vesicles and in particular microvesicles and exosomes, which are constitutively produced by cells, is on the rise for their huge...
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SubjectTerms Animals
Biomarkers - analysis
biosensors
Body Fluids - cytology
Cell-Derived Microparticles - chemistry
Cell-Derived Microparticles - metabolism
exosomes
Exosomes - chemistry
Exosomes - metabolism
extracellular vesicles
Humans
Lab-On-A-Chip Devices
lab-on-chip
microfabrication
microfluidics
Review
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Title Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization
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