Production and characterization of bacterial cellulose by Leifsonia sp. CBNU-EW3 isolated from the earthworm, Eisenia fetida

• Published in: Biotechnology and bioprocess engineering Vol. 20; no. 3; pp. 410 - 416
• Main Authors: Velmurugan, Palanivel, Myung, Hyun, Govarthanan, Muthusamy, Yi, Young-Joo, Seo, Sang-Ki, Cho, Kwang-Min, Lovanh, Nanh, Oh, Byung-Taek
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.06.2015; Springer Nature B.V; 한국생물공학회
• Subjects: Agricultural biotechnology; Bacteria; Biotechnology; Carbon; Cellulose; Chemistry; Chemistry and Materials Science; earthworms; Eisenia fetida; Fourier transform infrared spectroscopy; Fourier transforms; Glucose; Industrial and Production Engineering; Infrared spectroscopy; Leifsonia; Morphology; Nitrogen; Nitrogen sources; nucleotide sequences; Optimization; Pore size; porosity; Research Paper; ribosomal DNA; Scanning electron microscopy; sequence analysis; Soil microorganisms; Spectrum analysis; Studies; Worms; X-ray diffraction; Yeast; yeast extract; Yeasts; 생물공학; South Korea; optimization; bacterial cellulose; sp; characterization
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-014-0793-y

A total of five bacterial strains were isolated from earthworm, Eisenia fetida and examined for bacterial cellulose (BC) production in Hestrin-Schramm medium (HS). Among the five strains tested, CBNU-EW3 exhibited excellent BC production and was identified as Leifsonia sp. by 16S rDNA sequence analysis. BC production by Leifsonia sp. CBNU-EW3 was optimum at pH 5, 30°C, and with glucose and yeast extract as carbon and nitrogen sources, respectively, according to 15 day-long experiments. (XRD) analysis of the dried pellicle indicated that the BC was partially crystalline type I. Fourier transform infrared spectroscopy (FT-IR) analysis showed that the obtained pellicle contained the same functional groups as typical BC. Field emission scanning electron microscopy (FESEM) images showed that the BC micro-fibril matrix consisted of a flat surface with large pore size and cellulose aggregation.