Expression of suppressor of cytokine signaling 1 in the peripheral blood of patients with idiopathic pulmonary fibrosis

• Published in: Chinese medical journal Vol. 127; no. 11; pp. 2117 - 2120
• Main Authors: Bao, Zhiyao, Zhang, Qiurui, Wan, Huanying, He, Ping, Zhou, Xin, Zhou, Min
• Format: Journal Article
• Language: English
• Published: China Department of Respiratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China 2014; Department of Respiratory Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai 200080, China%Department of Respiratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China%Department of Medical Microbiology and Parasitology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China%Department of Respiratory Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai 200080, China
• Subjects: Adult; Aged; Aged, 80 and over; Enzyme-Linked Immunosorbent Assay; Female; Humans; Idiopathic Pulmonary Fibrosis - metabolism; Interleukin-4 - metabolism; Leukocytes, Mononuclear - metabolism; Male; Middle Aged; mRNA表达水平; Suppressor of Cytokine Signaling 1 Protein; Suppressor of Cytokine Signaling Proteins - genetics; Suppressor of Cytokine Signaling Proteins - metabolism; Transforming Growth Factor beta1 - metabolism; 信号传导; 外周血单个核细胞; 外周血单核细胞; 患者; 细胞因子; 肺纤维化; 酶联免疫吸附试验; suppressor of cytokine signaling; interleukin 4; idiopathic pulmonary fibrosis
• ISSN: 0366-6999; 2542-5641; 2542-5641
• DOI: 10.3760/cma.j.issn.0366-6999.20133038

Background Idiopathic pulmonary fibrosis （IPF） is a progressive diffuse parenchymal disease with a poor prognosis.A variety of cytokines and chemokines are involved in its pathophysiology.The aim of this study was to evaluate the clinical features in IPF patients with the expression of suppressor of cytokine signaling 1 （SOCS-1）,which acts as a negative regulator of cytokine signaling.Methods IPF patients （n=20） and healthy controls （n=16） were included in this study.The expression of SOCS-1 was analyzed in peripheral blood mononuclear cells （PBMC） of subjects using RT-PCR.Interleukin 4 （IL-4）,transforming growth factor β1 （TGF-β1） and type Ⅰ collagen expression were also analyzed in each individual using enzyme-linked immunosorbent assay （ELISA）.The clinical characteristics of IPF patients were delineated.These results were analyzed by SPSS13.0 statistics software.Results SOCS-1 mRNA expression was significantly decreased in the PBMC of IPF patients compared with healthy controls; serum levels of IL-4 and TGF-β1 were higher in IPF patients.The patients with lower expression of SOCS-1 developed lower percentage of forced vital capacity （FVC%） and DLCO/VA.A patients＇ SOCS-1 mRNA level was negatively correlated with serum levels of IL-4,and negatively correlated with their high-resolution computed tomography （HRCT） scores.Conclusions SOCS-1 mRNA can be detected in PBMC,and it is down-regulated in IPF patients.The expression of SOCS-1 is associated with the severity of IPF patients＇ symptoms,so it might be the predictor of disease severity.SOCS-1 might play an important role in IPF by reducing the expression of the T helper type 2 （Th2） cell-related cytokine IL-4.