Correlation and Expression of COX-2 and P53 Protein in Basal Cell Carcinoma of Eyelid

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 29; no. 3; pp. 383 - 386
• Main Author: 陈志雄 杨炯 黄琼
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.06.2009; Department of Geriatrics, Renmin Hospital of Wuhan University, Wuhan 430060, China%Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, China
• Subjects: Adult; Aged; Apoptosis; Carcinoma, Basal Cell - metabolism; COX-2; Cyclooxygenase 2 - metabolism; Eyelid Neoplasms - metabolism; Female; Humans; Male; Medicine; Medicine & Public Health; Middle Aged; Tumor Suppressor Protein p53 - metabolism; 癌细胞; basal cell carcinoma; eyelid; COX-2; p53 protein
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-009-0324-9

The correlation between the expression of COX-2 and p53 protein in basal cell carcinoma （BCC） of eyelid and apoptosis was investigated. Specimens of BCC were collected from 40 cases （aged 28-68 y） at the Department of Pathology, Renmin Hospital of Wuhan University, and Department of Pathology, Zhongnan Hospital of Wuhan University during from 1999 to 2006. Five specimens of paracancerous tissues served as control group. Immunohistochemical staining was performed to detect the expression of COX-2 and p53 in the tissues. The average absorbance （A） and the average positive area rate of COX-2 and p53 protein were measured by image analysis. The positive area rate of COX-2 and p53 protein was analyzed by linear correlation analysis. It was found that COX-2 and p53 proteins were highly expressed in BCC of eyelid, and weakly expressed in paracancerous tissues. Image analysis revealed that the expression of COX-2 and p53 proteins in BCC of eyelid was sig- nificantly higher than that in paracancerous tissues （P〈0.01）. Spearman rank correlation analysis demonstrated a positive correlation between the expression of COX-2 and p53 （r=0.113, P=0.421）. It was concluded that COX-2 can increase the expression of p53 protein, therefore suppressing apoptosis.