Microbiological and immunological characteristics of young Moroccan patients with aggressive periodontitis with and without detectable Aggregatibacter actinomycetemcomitans JP2 infection

• Published in: Molecular oral microbiology Vol. 26; no. 1; pp. 35 - 51
• Main Authors: Rylev, M., Bek-Thomsen, M., Reinholdt, J., Ennibi, O.-K., Kilian, M.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.02.2011
• Subjects: Actinobacillus Infections - immunology; Actinobacillus Infections - microbiology; Adolescence; Adolescent; aetiology; Aggregatibacter actinomycetemcomitans - classification; Aggregatibacter actinomycetemcomitans - genetics; Aggregatibacter actinomycetemcomitans - immunology; aggressive periodontitis; Aggressive Periodontitis - immunology; Aggressive Periodontitis - microbiology; Antibodies; Antibodies, Bacterial - blood; Bacterial Toxins - immunology; Case-Control Studies; Clone Cells; Disease Susceptibility - immunology; Exotoxins - immunology; Gingival Hemorrhage - immunology; Gingival Hemorrhage - microbiology; Humans; Immunity; Immunosuppressive Agents - immunology; Infection; leukotoxin; Morocco; Nucleic Acid Amplification Techniques; Pathogens; Periodontal Attachment Loss - immunology; Periodontal Attachment Loss - microbiology; Periodontal diseases; Periodontal Pocket - immunology; Periodontal Pocket - microbiology; Periodontitis; Phylogeny; Polymerase Chain Reaction; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Young Adult; Morocco
• ISSN: 2041-1006; 2041-1014; 2041-1014
• DOI: 10.1111/j.2041-1014.2010.00593.x

Summary Cross‐sectional and longitudinal studies identify the JP2 clone of Aggregatibacter actinomycetemcomitans as an aetiological agent of aggressive periodontitis (AgP) in adolescents of northwest African descent. To gain information on why a significant part of Moroccan adolescents show clinical signs of periodontal disease in the absence of this pathogen we performed comprehensive mapping of the subgingival microbiota of eight young Moroccans, four of whom were diagnosed with clinical signs of AgP. The analysis was carried out by sequencing and phylogenetic analysis of a total of 2717 cloned polymerase chain reaction amplicons of the phylogenetically informative 16S ribosomal RNA gene. The analyses revealed a total of 173 bacterial taxa of which 39% were previously undetected. The JP2 clone constituted a minor proportion of the complex subgingival microbiota in patients with active disease. Rather than identifying alternative aetiologies to AgP, the recorded infection history of the subjects combined with remarkably high concentrations of antibodies against the A. actinomycetemcomitans leukotoxin suggest that disease activity was terminated in some patients with AgP as a result of elimination of the JP2 clone. This study provides information on the microbial context of the JP2 clone activity in a JP2‐susceptible population and suggests that such individuals may develop immunity to AgP.