Involvement of spinal muscarinic and serotonergic receptors in the anti-allodynic effect of electroacupuncture in rats with oxaliplatin-induced neuropathic pain

This study was performed to investigate whether the spinal cholinergic and serotonergic analgesic systems mediate the relieving effect of electroacupuncture (EA) on oxaliplatin-induced neuropathic cold allodynia in rats. The cold allodynia induced by an oxaliplatin injection (6 mg/kg, i.p.) was eval...

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Published inThe Korean journal of physiology & pharmacology Vol. 20; no. 4; pp. 407 - 414
Main Authors Lee, Ji Hwan, Go, Donghyun, Kim, Woojin, Lee, Giseog, Bae, Hyojeong, Quan, Fu Shi, Kim, Sun Kwang
Format Journal Article
LanguageEnglish
Published Korea (South) The Korean Physiological Society and The Korean Society of Pharmacology 01.07.2016
대한약리학회
Subjects
Original
약리학
Acetylcholine
Oxaliplatin
Cold allodynia
Electroacupuncture
Serotonin
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ISSN1226-4512
2093-3827
2093-3827
DOI10.4196/kjpp.2016.20.4.407

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Summary:This study was performed to investigate whether the spinal cholinergic and serotonergic analgesic systems mediate the relieving effect of electroacupuncture (EA) on oxaliplatin-induced neuropathic cold allodynia in rats. The cold allodynia induced by an oxaliplatin injection (6 mg/kg, i.p.) was evaluated by immersing the rat's tail into cold water (4℃) and measuring the withdrawal latency. EA stimulation (2 Hz, 0.3-ms pulse duration, 0.2~0.3 mA) at the acupoint ST36, GV3, or LI11 all showed a significant anti-allodynic effect, which was stronger at ST36. The analgesic effect of EA at ST36 was blocked by intraperitoneal injection of muscarinic acetylcholine receptor antagonist (atropine, 1 mg/kg), but not by nicotinic (mecamylamine, 2 mg/kg) receptor antagonist. Furthermore, intrathecal administration of M2 (methoctramine, 10 µg) and M3 (4-DAMP, 10 µg) receptor antagonist, but not M1 (pirenzepine, 10 µg) receptor antagonist, blocked the effect. Also, spinal administration of 5-HT3 (MDL-72222, 12 µg) receptor antagonist, but not 5-HT1A (NAN-190, 15 µg) or 5-HT2A (ketanserin, 30 µg) receptor antagonist, prevented the anti-allodynic effect of EA. These results suggest that EA may have a signifi cant analgesic action against oxaliplatin-induced neuropathic pain, which is mediated by spinal cholinergic (M2, M3) and serotonergic (5-HT3) receptors.
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These authors contributed equally to this work.
G704-000764.2016.20.4.002
ISSN:1226-4512
2093-3827
2093-3827
DOI:10.4196/kjpp.2016.20.4.407