Interassay Correlation of Human Herpesvirus 8 Serologic Tests

To standardize human herpesvirus 8 (HHV-8) antibody assays for application to asymptomatic infection, a blinded comparison was done of seven immunofluorescence assays and ELISAs. Five experienced laboratories tested a serum panel from 143 subjects in 4 diagnostic groups. Except for a minor capsid pr...

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Published inThe Journal of infectious diseases Vol. 178; no. 2; pp. 304 - 309
Main Authors Rabkin, Charles S., Schulz, Thomas F., Whitby, Denise, Lennette, Evelyne T., Magpantay, Larry I., Chatlynne, Louise, Biggar, Robert J.
Format Journal Article
LanguageEnglish
Published Chicago, IL The University of Chicago Press 01.08.1998
University of Chicago Press
Oxford University Press
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Online AccessGet full text
ISSN0022-1899
1537-6613
DOI10.1086/515649

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Abstract To standardize human herpesvirus 8 (HHV-8) antibody assays for application to asymptomatic infection, a blinded comparison was done of seven immunofluorescence assays and ELISAs. Five experienced laboratories tested a serum panel from 143 subjects in 4 diagnostic groups. Except for a minor capsid protein ELISA, the other six tests detected HHV-8 antibodies most frequently in classic (80%–100%) and AIDS-related (67%–91%) Kaposi's sarcoma, followed by human immunodeficiency virus-seropositive patients (27%–60%), and least frequently in healthy blood donors (0–29%). However, these six assays frequently disagreed on individual sera, particularly for blood donor samples. Current HHV-8 antibody tests have uncertain accuracy in asymptomatic HHV-8 infection and may require correlation with viral protein or nucleic acid detection. Antibody assays are useful for epidemiologic investigations, but the absolute prevalence of HHV-8 infection in the United States cannot yet be determined.
AbstractList To standardize human herpesvirus 8 (HHV-8) antibody assays for application to asymptomatic infection, a blinded comparison was done of seven immunofluorescence assays and ELISAs. Five experienced laboratories tested a serum panel from 143 subjects in 4 diagnostic groups. Except for a minor capsid protein ELISA, the other six tests detected HHV-8 antibodies most frequently in classic (80%-100%) and AIDS-related (67%-91%) Kaposi's sarcoma, followed by human immunodeficiency virus-seropositive patients (27%-60%), and least frequently in healthy blood donors (0-29%). However, these six assays frequently disagreed on individual sera, particularly for blood donor samples. Current HHV-8 antibody tests have uncertain accuracy in asymptomatic HHV-8 infection and may require correlation with viral protein or nucleic acid detection. Antibody assays are useful for epidemiologic investigations, but the absolute prevalence of HHV-8 infection in the United States cannot yet be determined.
Author Whitby, Denise
Biggar, Robert J.
Lennette, Evelyne T.
Schulz, Thomas F.
Chatlynne, Louise
Magpantay, Larry I.
Rabkin, Charles S.
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  fullname: Lennette, Evelyne T.
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  surname: Magpantay
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Issue 2
Keywords Performance evaluation
Human
Skin disease
Antibody
Herpesviridae
Serology
Malignant tumor
Method
Infection
Virus
Kaposi sarcoma
Viral disease
Diagnosis
ELISA assay
Comparative study
Language English
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Notes Reprints or correspondence: Dr. Charles S. Rabkin, Viral Epidemiology Branch, NCI, 6130 Executive Blvd., EPN/434, Rockville, MD 20852.
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Presented in part: 4th Conference on Retroviruses and Opportunistic Infections, Washington, DC, 22–26 January 1997 (abstract 699).
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Snippet To standardize human herpesvirus 8 (HHV-8) antibody assays for application to asymptomatic infection, a blinded comparison was done of seven immunofluorescence...
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StartPage 304
SubjectTerms Antibodies
Antigens
Biological and medical sciences
Blood donation
Capsid proteins
Enzyme linked immunosorbent assay
Fundamental and applied biological sciences. Psychology
Herpesviridae
Human herpesvirus 8
Human viral diseases
Infectious diseases
Kaposi sarcoma
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Medical sciences
Microbiology
Open reading frames
Techniques used in virology
Viral diseases
Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye
Virology
Viruses
Title Interassay Correlation of Human Herpesvirus 8 Serologic Tests
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