Interassay Correlation of Human Herpesvirus 8 Serologic Tests
To standardize human herpesvirus 8 (HHV-8) antibody assays for application to asymptomatic infection, a blinded comparison was done of seven immunofluorescence assays and ELISAs. Five experienced laboratories tested a serum panel from 143 subjects in 4 diagnostic groups. Except for a minor capsid pr...
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Published in | The Journal of infectious diseases Vol. 178; no. 2; pp. 304 - 309 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Chicago, IL
The University of Chicago Press
01.08.1998
University of Chicago Press Oxford University Press |
Subjects | |
Online Access | Get full text |
ISSN | 0022-1899 1537-6613 |
DOI | 10.1086/515649 |
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Abstract | To standardize human herpesvirus 8 (HHV-8) antibody assays for application to asymptomatic infection, a blinded comparison was done of seven immunofluorescence assays and ELISAs. Five experienced laboratories tested a serum panel from 143 subjects in 4 diagnostic groups. Except for a minor capsid protein ELISA, the other six tests detected HHV-8 antibodies most frequently in classic (80%–100%) and AIDS-related (67%–91%) Kaposi's sarcoma, followed by human immunodeficiency virus-seropositive patients (27%–60%), and least frequently in healthy blood donors (0–29%). However, these six assays frequently disagreed on individual sera, particularly for blood donor samples. Current HHV-8 antibody tests have uncertain accuracy in asymptomatic HHV-8 infection and may require correlation with viral protein or nucleic acid detection. Antibody assays are useful for epidemiologic investigations, but the absolute prevalence of HHV-8 infection in the United States cannot yet be determined. |
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AbstractList | To standardize human herpesvirus 8 (HHV-8) antibody assays for application to asymptomatic infection, a blinded comparison was done of seven immunofluorescence assays and ELISAs. Five experienced laboratories tested a serum panel from 143 subjects in 4 diagnostic groups. Except for a minor capsid protein ELISA, the other six tests detected HHV-8 antibodies most frequently in classic (80%-100%) and AIDS-related (67%-91%) Kaposi's sarcoma, followed by human immunodeficiency virus-seropositive patients (27%-60%), and least frequently in healthy blood donors (0-29%). However, these six assays frequently disagreed on individual sera, particularly for blood donor samples. Current HHV-8 antibody tests have uncertain accuracy in asymptomatic HHV-8 infection and may require correlation with viral protein or nucleic acid detection. Antibody assays are useful for epidemiologic investigations, but the absolute prevalence of HHV-8 infection in the United States cannot yet be determined. |
Author | Whitby, Denise Biggar, Robert J. Lennette, Evelyne T. Schulz, Thomas F. Chatlynne, Louise Magpantay, Larry I. Rabkin, Charles S. |
Author_xml | – sequence: 1 givenname: Charles S. surname: Rabkin fullname: Rabkin, Charles S. organization: Viral Epidemiology Branch, National Cancer Institute, Bethesda – sequence: 2 givenname: Thomas F. surname: Schulz fullname: Schulz, Thomas F. organization: Viral Epidemiology Branch, National Cancer Institute, Bethesda – sequence: 3 givenname: Denise surname: Whitby fullname: Whitby, Denise organization: Viral Epidemiology Branch, National Cancer Institute, Bethesda – sequence: 4 givenname: Evelyne T. surname: Lennette fullname: Lennette, Evelyne T. organization: Viral Epidemiology Branch, National Cancer Institute, Bethesda – sequence: 5 givenname: Larry I. surname: Magpantay fullname: Magpantay, Larry I. organization: Viral Epidemiology Branch, National Cancer Institute, Bethesda – sequence: 6 givenname: Louise surname: Chatlynne fullname: Chatlynne, Louise organization: Viral Epidemiology Branch, National Cancer Institute, Bethesda – sequence: 7 givenname: Robert J. surname: Biggar fullname: Biggar, Robert J. organization: Viral Epidemiology Branch, National Cancer Institute, Bethesda |
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Keywords | Performance evaluation Human Skin disease Antibody Herpesviridae Serology Malignant tumor Method Infection Virus Kaposi sarcoma Viral disease Diagnosis ELISA assay Comparative study |
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Notes | Reprints or correspondence: Dr. Charles S. Rabkin, Viral Epidemiology Branch, NCI, 6130 Executive Blvd., EPN/434, Rockville, MD 20852. istex:4FF1B8AFFCF2F0A37D84047CCC67DBE75CFA5AFB ark:/67375/HXZ-LK36B9VW-H Presented in part: 4th Conference on Retroviruses and Opportunistic Infections, Washington, DC, 22–26 January 1997 (abstract 699). ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Article-2 ObjectType-Feature-1 content type line 23 |
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SubjectTerms | Antibodies Antigens Biological and medical sciences Blood donation Capsid proteins Enzyme linked immunosorbent assay Fundamental and applied biological sciences. Psychology Herpesviridae Human herpesvirus 8 Human viral diseases Infectious diseases Kaposi sarcoma Major Articles Medical sciences Microbiology Open reading frames Techniques used in virology Viral diseases Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye Virology Viruses |
Title | Interassay Correlation of Human Herpesvirus 8 Serologic Tests |
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