Tetrabromobisphenol A Promotes the Osteoclastogenesis of RAW264.7 Cells Induced by Receptor Activator of NF-kappa B Ligand In Vitro

• Published in: Journal of Korean medical science Vol. 34; no. 41; pp. e267 - 12
• Main Authors: Park, So Young, Choi, Eun Mi, Suh, Kwang Sik, Kim, Hyun-Sook, Chin, Sang Ouk, Rhee, Sang Youl, Kim, Deog-Yoon, Oh, Seungjoon, Chon, Suk
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Academy of Medical Sciences 28.10.2019; 대한의학회
• Subjects: Animals; Cell Differentiation - drug effects; Membrane Potential, Mitochondrial - drug effects; Mice; NFATC Transcription Factors - metabolism; Original; Osteoclasts - cytology; Osteoclasts - metabolism; Osteogenesis - drug effects; Polybrominated Biphenyls - pharmacology; Proto-Oncogene Proteins c-akt - metabolism; RANK Ligand - pharmacokinetics; RAW 264.7 Cells; Reactive Oxygen Species - metabolism; Tartrate-Resistant Acid Phosphatase - metabolism; 의학일반; Osteoclastogenesis; Tetrabromobisphenol A; RAW264.7 Cells; Mitochondrial Function
• ISSN: 1011-8934; 1598-6357; 1598-6357
• DOI: 10.3346/jkms.2019.34.e267

Tetrabromobisphenol A (TBBPA), one of the most widely used brominated flame-retardants, is a representative persistent organic pollutants group. Studies on TBBPA toxicity have been conducted using various target cells; however, few studies have investigated TBBPA toxicity in bone cells. Therefore, this study investigated the in vitro effects of TBBPA on osteoclasts, a cell type involved in bone metabolism. RAW264.7 cells were cultured in medium containing 50 ng/mL receptor activator of nuclear factor kappa B ligand (RANKL) and varying concentrations of TBBPA. To evaluate the effects of TBBPA on the differentiation and function of osteoclasts, osteoclast-specific gene expression, tartrate-resistant acid phosphatase (TRAP) activity, bone resorbing activity, mitochondrial membrane potential (MMP) and mitochondrial superoxide were measured. The presence of 20 μ TBBPA significantly increased TRAP activity in RANKL-stimulated RAW264.7 cells, the bone resorbing activity of osteoclasts, and the gene expression of Akt2, nuclear factor of activated T-cells cytoplasmic 1, and chloride channel voltage-sensitive 7. However, TBBPA treatment caused no change in the expression of carbonic anhydrase II, cathepsin K, osteopetrosis-associated transmembrane protein 1, , extracellular signal-related kinase, , , or matrix metalloproteinase 9. Furthermore, 20 μ TBBPA caused a significant decrease in MMP and a significant increase in mitochondrial superoxide production. This study suggests that TBBPA promotes osteoclast differentiation and activity. The mechanism of TBBPA-stimulated osteoclastogenesis might include increased expression of several genes involved in osteoclast differentiation and reactive oxygen species production.