Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus

• Published in: Chinese journal of oceanology and limnology Vol. 33; no. 2; pp. 309 - 318
• Main Author: 李成泽 常亚青 庞震国 丁君 姬南京
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer-Verlag 01.03.2015; Science Press; Springer Nature B.V
• Subjects: Abiotic stress; acute exposure; Ambient temperature; Apostichopus japonicus; Aquatic life; Biology; cDNA libraries; cold stress; Cold tolerance; Complementary DNA; Earth and Environmental Science; Earth Sciences; Environmental conditions; Ferritin; Gene expression; gene expression regulation; Gene regulation; Genes; Glycoprotein gp96; growth and development; Holothurioidea; Hsp70 protein; Hybridization; Intestine; Intestines; Low temperature; Lysozyme; Marine invertebrates; messenger RNA; muscles; Oceanography; reproduction; respiratory system; Skin diseases; suppression subtractive hybridization; Survival; Temperature tolerance; Tissue; tissues; Tolerance; transcription (genetics); Transcription factors; Up-regulation; 刺参; 抑制性消减杂交; 治疗; 海参; 热休克蛋白70; 环境温度; 相关基因; 耐寒; INW, Japan; low temperature stress; transcriptional regulation; gene expression
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-015-4148-0

Environmental conditions, including ambient temperature, play important roles in survival growth development, and reproduction of the Japanese sea cucumber, Apostichopus japonicus. Low temperatures result in slowed growth and skin ulceration disease. In a previous study, we investigated the effect of low temperature on gene expression profiles inA.japonicus by suppression subtractive hybridization （SSH）. Genes encoding Ferritin, Lysozyme, Hsp70, gp96, and AjToll were selected from a subtracted cDNA library of A. japonicus under acute cold stress. The transcriptional expression profiles of these genes were investigated in different tissues （coelomocyte, respiratory tree, intestine, longitudinal muscle） after exposure to acute and mild temperature dropping treatments. The results show that （1） the five cold-tolerance-related genes were found in all four tissues and the highest mRNA levels were observed in coelomocyte and respiratory tree; （2） under the temperature dropping treatments, three types of transcriptional regulation patterns were observed： primary suppression followed by up-regulation at -2℃, suppressed expression throughout the two treatments, and more rarely an initial stimulation followed by suppression; and （3） gene expression suppression was more severe under acute temperature dropping than under mild temperature dropping treatment. The five cold-tolerance-related genes that were ,distributed mainly in coelomocyte and respiratory tissues were generally down-regulated by low temperature stress but an inverse up-regulation event was found at the extreme temperature （-2℃）.