Fluxes of the Amazon River plume nutrients and microbes into marine sponges

• Published in: The Science of the total environment Vol. 847; p. 157474
• Main Authors: de Menezes, Tatiane A., de Freitas, Mayanne A.M., Lima, Michele S., Soares, Ana Carolina, Leal, Camille, Busch, Mileane de S., Tschoeke, Diogo A., de O. Vidal, Luciana, Atella, Georgia C., Kruger, Ricardo H., Setubal, João, Vasconcelos, Agnaldo A., de Mahiques, Michel M., Siegle, Eduardo, Asp, Nils Edvin, Cosenza, Carlos, Hajdu, Eduardo, de Rezende, Carlos E., Thompson, Cristiane C., Thompson, Fabiano L.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.11.2022
• Subjects: Amazon River; bacteriophages; Biomarkers; coevolution; electron transfer; environment; Isotopic features; Lipidomics; Marine sponges; Metagenomics; microbiome; nutrition; photophosphorylation; Prochlorococcus; river plume; sediments; Synechococcus; water quality; Amazon River; Marine sponges; Biomarkers; Metagenomics; Lipidomics; Isotopic features
• ISSN: 0048-9697; 1879-1026; 1879-1026
• DOI: 10.1016/j.scitotenv.2022.157474

Sponges have co-evolved with microbes for over 400 myr. Previous studies have demonstrated that sponges can be classified according to the abundance of microbes in their tissues as Low Microbial Abundance (LMA) and High Microbial Abundance (HMA). While LMA sponges rely mainly on water column microbes, HMA appear to rely much more on symbiotic fermentative and autotrophic microbes maintained in their tissues. However, it is unclear if this pattern holds when comparing different species of tropical sponges under extreme nutrient conditions and sediment loads in the water column, such as the Great Amazon Reef System (GARS), which covers an area of ~56,000 km2 off the Amazon River mouth. Sponges are the major GARS benthic components. However, these sponges' microbiome across the GARS is still unknown. Here, we investigated water quality, isotopic values (δ13C and δ15N), metagenomic and lipidomic profiles of sponges obtained from different sectors throughout the GARS. >180 million shotgun metagenomic reads were annotated, covering 22 sponge species. Isotopic and lipidomic analyses suggested LMA sponges rely on the Amazon River Plume for nutrition. HMA sponges (N = 15) had higher Roseiflexus and Nitrospira abundance, whereas LMA sponges (N = 7) had higher Prochlorococcus and Pelagibacter abundance. Functional data revealed that the LMA sponge microbiomes had greater number of sequences related to phages and prophages as well as electron transport and photophosphorylation which may be related to photosynthetic processes associated with the Prochlorococcus and Synechococcus found in the LMA. The higher phages abundance in LMA sponges could be related to these holobionts' reduced defense towards phage infection. Meanwhile, HMA sponge microbiomes had higher Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR abundance, which may be involved in defense against phage infection. This study sheds light on the nutrient fluxes and microbes from the Amazon River plume into the sponge holobionts. [Display omitted] •The river, plume and ocean organic matter have similar isotopic signature.•Plume microbes (DDAs) are also found in the sponge holobionts.•Fluxes of matter and energy from Amazon river into GARS sponges•Sponges are split into 2 distinct groups (LMA and HMA).•HMA sponge symbionts engaged in N cycling.