Phytohormone based biostimulant combined with plant growth promoting endophytic fungus enhances Ni phytoextraction of Noccaea goesingensis

• Published in: The Science of the total environment Vol. 789; p. 147950
• Main Authors: Ważny, Rafał, Rozpądek, Piotr, Jędrzejczyk, Roman J., Domka, Agnieszka, Nosek, Michał, Kidd, Petra, Turnau, Katarzyna
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.10.2021
• Subjects: Agromining; biofertilizers; biomass; biometry; Cytokinin; Diaporthe; DNA; endophytes; environment; fungi; hyperaccumulators; Hyperacumulators; IAA; mycelium; Noccaea; peptide elongation factors; Phomopsis columnaris; phytoaccumulation; plant extracts; plant growth; Agromining; Hyperacumulators; IAA; Phomopsis columnaris; Cytokinin
• ISSN: 0048-9697; 1879-1026; 1879-1026
• DOI: 10.1016/j.scitotenv.2021.147950

To improve the efficiency of Ni phytoextraction, the metal hyperaccumulator N. goesingensis was subject to treatment with a combination of a Ni uptake stimulating microorganism and the commercially available, IAA- based biostimulating seaweed extract - Kelpak. Additionally, we compared the plant growth promoting and Ni uptake capabilities of the two biofertilizers. Treatment with the Kelpak alone had no significant effect on plant growth or Ni accumulation. Inoculation of N. goesingensis with Phomopsis columnaris significantly improved the biomass of the hyperaccumulating plant and Ni yield per plant and improved several plant biometric features such as fresh and dry weight and several others related to leaf and root size. However, the combination of the two treatments yielded the best results; plants treated with the two growth promoting agents yielded 85% more biomass compared to not treated plants and accumulated 48% more Ni per plant. To verify plant inoculation with the fungus we generated a GFP expressing strain of P. columnaris and visualized the fungus in both plant leaves and roots. To trace the development of the fungus in planta and to evaluate the effect of biostimulant treatment on mycelium development fungal translational elongation factor 1α (tef1α) DNA was quantified with qPCR. Upon biofertilizer the abundance P. columnaris in plant leaves increased nearly 5-fold. The utilization of plant growth stimulating microorganisms, endophytic fungi in particular, can significantly improve Ni phytoextraction in hyperaccumulator N. goesingensis. [Display omitted] •P. columnaris stimulated N. goesingensis growth in Ni-enriched substrate.•Kelpak and the fungus increased plant biomass yield by 85%.•The IAA-based biostimulant increased fungal abundance in plant leaves nearly 5-fold.•The biostimulant and fungus inoculation increased plant Ni accumulation by 48%.