A study of inulinase production in Aspergillus niger using fractional factorial design

• Published in: Bioresource technology Vol. 54; no. 3; pp. 315 - 320
• Main Authors: Poorna, V., Kulkarni, P.R.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1995; Elsevier Science
• Subjects: A. niger; Aspergillus niger; Biological and medical sciences; Biotechnology; culture media; Enzyme engineering; experimental design; Fermentation; fractional factorial design; Fundamental and applied biological sciences. Psychology; inulinase; Methods. Procedures. Technologies; one-factor-at-a-time; process parameters; Production of selected enzymes; one-factor-at-a-time; A. niger; process parameters; fractional factorial design; Parametrization; Carbon compound; Enzyme; Applied microbiology; Fractional step method; Fermentation; Fungi; Enzymatic activity; Aspergillus niger; Glycosidases; Inulinase; Microorganism culture; Hydrolases; Carbohydrate; Fungi Imperfecti; Inuline; Factorial design; O-Glycosidases; Polysaccharide; Thallophyta
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/0960-8524(95)00156-5

The effects of process parameters on inulinase production by Aspergillus niger van Teighem have been studied by one-factor-at-a-time and fractional design. Use of various carbon sources, singly and in combination, suggested that inulinase production by Aspergillus niger van Teighem is probably inducible and subject to catabolite repression. A fractional factorial method using the Plackett-Burman design showed that inulin, among the various carbon sources studied, had the highest positive influence on inulinase production.