A simple and rapid DNA extraction method from meat by microneedle patch for detection of adulterated components

The simple and rapid identification of meat via DNA-based methods is still subject to the conditions of complex and time-consuming preprocessing. It usually takes 3–4 h to obtain DNA sample using conventionally available DNA extraction kits. The existing approaches are cumbersome, require large equi...

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Bibliographic Details
Published inFood and agricultural immunology Vol. 34; no. 1
Main Authors Hu, Weilun, Xu, Mengxin, Zeng, Qingya, Li, Yingying, Zhang, Yuxin, Wang, Qingqing, Ma, Tao
Format Journal Article
LanguageEnglish
Published Abingdon Taylor & Francis Ltd 31.12.2023
Taylor & Francis Group
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ISSN0954-0105
1465-3443
1465-3443
DOI10.1080/09540105.2023.2248412

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Summary:The simple and rapid identification of meat via DNA-based methods is still subject to the conditions of complex and time-consuming preprocessing. It usually takes 3–4 h to obtain DNA sample using conventionally available DNA extraction kits. The existing approaches are cumbersome, require large equipment, and are not suitable for field operation. This study developed a swelling microneedle patch, constituted by micron-sized copolymers of methyl vinyl ether and maleic acid (PMVE/MA). The microneedle patch was applied to the meat sample for 1 min before being pulled out and rinsed with Tris-EDTA (TE) buffer to obtain DNA, which could be used for polymerase chain reaction (PCR) without purification. The DNA extracted by the microneedle patch could meet the needs for amplification and identification of meat samples. The developed approach which combines the patch-based extraction method and conventional PCR amplification is quick, simple, and reliable for DNA extraction and identification of meat samples.
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ISSN:0954-0105
1465-3443
1465-3443
DOI:10.1080/09540105.2023.2248412