Comparison of indirect fluorescent antibody test and modified agglutination test for detecting Toxoplasma gondii immunoglobulin G antibodies in dog and cat
The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of Toxoplasma specific IgG antibodies in dog and cat sera. MAT showed an “almost perfect” agreement with IFAT in detecting positive and negative...
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Published in | Parasitology research (1987) Vol. 105; no. 1; pp. 35 - 40 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
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Berlin/Heidelberg
Berlin/Heidelberg : Springer-Verlag
01.07.2009
Springer-Verlag Springer |
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ISSN | 0932-0113 1432-1955 1432-1955 |
DOI | 10.1007/s00436-009-1358-4 |
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Abstract | The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of Toxoplasma specific IgG antibodies in dog and cat sera. MAT showed an “almost perfect” agreement with IFAT in detecting positive and negative results in cat sera, where as only a “substantial” agreement was observed in dog sera due to false negative results. Differences relative to sample dilution were recorded and serological titres obtained by MAT and IFAT are not directly comparable in cat and dog sera. |
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AbstractList | The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of Toxoplasma specific IgG antibodies in dog and cat sera. MAT showed an “almost perfect” agreement with IFAT in detecting positive and negative results in cat sera, where as only a “substantial” agreement was observed in dog sera due to false negative results. Differences relative to sample dilution were recorded and serological titres obtained by MAT and IFAT are not directly comparable in cat and dog sera. The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of Toxoplasma specific IgG antibodies in dog and cat sera. MAT showed an "almost perfect" agreement with IFAT in detecting positive and negative results in cat sera, where as only a "substantial" agreement was observed in dog sera due to false negative results. Differences relative to sample dilution were recorded and serological titres obtained by MAT and IFAT are not directly comparable in cat and dog sera.The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of Toxoplasma specific IgG antibodies in dog and cat sera. MAT showed an "almost perfect" agreement with IFAT in detecting positive and negative results in cat sera, where as only a "substantial" agreement was observed in dog sera due to false negative results. Differences relative to sample dilution were recorded and serological titres obtained by MAT and IFAT are not directly comparable in cat and dog sera. The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of Toxoplasma specific IgG antibodies in dog and cat sera. MAT showed an “almost perfect” agreement with IFAT in detecting positive and negative results in cat sera, where as only a “substantial” agreement was observed in dog sera due to false negative results. Differences relative to sample dilution were recorded and serological titres obtained by MAT and IFAT are not directly comparable in cat and dog sera. |
Author | Sala, Marcello Pettirossi, Nadia Macrì, Gladia Scarpulla, Manuela Linder, Alicia M |
Author_xml | – sequence: 1 fullname: Macrì, Gladia – sequence: 2 fullname: Sala, Marcello – sequence: 3 fullname: Linder, Alicia M – sequence: 4 fullname: Pettirossi, Nadia – sequence: 5 fullname: Scarpulla, Manuela |
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Keywords | True Prevalence Indirect Fluorescent Antibody Test Modify Agglutination Test Positive Seron Apparent Prevalence Fissipedia Protozoa Apicomplexa Carnivora Immunoglobulins Antibody Parasite Vertebrata Mammalia Toxoplasma gondii Cat Agglutination test Dog |
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SubjectTerms | Agglutination Tests Animals antibodies Antibodies, Protozoan Antibodies, Protozoan - blood Biological and medical sciences Biomedical and Life Sciences Biomedicine blood Cat Diseases Cat Diseases - diagnosis Cat Diseases - parasitology Cats diagnosis Dog Diseases Dog Diseases - diagnosis Dog Diseases - parasitology Dogs False Negative Reactions Fluorescent Antibody Technique, Indirect Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models immunoglobulin G Immunoglobulin G - blood Immunology Invertebrates Medical Microbiology Microbiology Original Paper parasitology Sensitivity and Specificity Toxoplasma Toxoplasma - immunology Toxoplasma gondii Toxoplasmosis, Animal Toxoplasmosis, Animal - diagnosis |
Title | Comparison of indirect fluorescent antibody test and modified agglutination test for detecting Toxoplasma gondii immunoglobulin G antibodies in dog and cat |
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