Comparison of indirect fluorescent antibody test and modified agglutination test for detecting Toxoplasma gondii immunoglobulin G antibodies in dog and cat

The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of Toxoplasma specific IgG antibodies in dog and cat sera. MAT showed an “almost perfect” agreement with IFAT in detecting positive and negative...

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Published inParasitology research (1987) Vol. 105; no. 1; pp. 35 - 40
Main Authors Macrì, Gladia, Sala, Marcello, Linder, Alicia M, Pettirossi, Nadia, Scarpulla, Manuela
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.07.2009
Springer-Verlag
Springer
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ISSN0932-0113
1432-1955
1432-1955
DOI10.1007/s00436-009-1358-4

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Abstract The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of Toxoplasma specific IgG antibodies in dog and cat sera. MAT showed an “almost perfect” agreement with IFAT in detecting positive and negative results in cat sera, where as only a “substantial” agreement was observed in dog sera due to false negative results. Differences relative to sample dilution were recorded and serological titres obtained by MAT and IFAT are not directly comparable in cat and dog sera.
AbstractList The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of Toxoplasma specific IgG antibodies in dog and cat sera. MAT showed an “almost perfect” agreement with IFAT in detecting positive and negative results in cat sera, where as only a “substantial” agreement was observed in dog sera due to false negative results. Differences relative to sample dilution were recorded and serological titres obtained by MAT and IFAT are not directly comparable in cat and dog sera.
The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of Toxoplasma specific IgG antibodies in dog and cat sera. MAT showed an "almost perfect" agreement with IFAT in detecting positive and negative results in cat sera, where as only a "substantial" agreement was observed in dog sera due to false negative results. Differences relative to sample dilution were recorded and serological titres obtained by MAT and IFAT are not directly comparable in cat and dog sera.The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of Toxoplasma specific IgG antibodies in dog and cat sera. MAT showed an "almost perfect" agreement with IFAT in detecting positive and negative results in cat sera, where as only a "substantial" agreement was observed in dog sera due to false negative results. Differences relative to sample dilution were recorded and serological titres obtained by MAT and IFAT are not directly comparable in cat and dog sera.
The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of Toxoplasma specific IgG antibodies in dog and cat sera. MAT showed an “almost perfect” agreement with IFAT in detecting positive and negative results in cat sera, where as only a “substantial” agreement was observed in dog sera due to false negative results. Differences relative to sample dilution were recorded and serological titres obtained by MAT and IFAT are not directly comparable in cat and dog sera.
Author Sala, Marcello
Pettirossi, Nadia
Macrì, Gladia
Scarpulla, Manuela
Linder, Alicia M
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Issue 1
Keywords True Prevalence
Indirect Fluorescent Antibody Test
Modify Agglutination Test
Positive Seron
Apparent Prevalence
Fissipedia
Protozoa
Apicomplexa
Carnivora
Immunoglobulins
Antibody
Parasite
Vertebrata
Mammalia
Toxoplasma gondii
Cat
Agglutination test
Dog
Language English
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Snippet The present study describes the comparison between a modified agglutination test (MAT) and the indirect fluorescent antibody test (IFAT) for the detection of...
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SubjectTerms Agglutination Tests
Animals
antibodies
Antibodies, Protozoan
Antibodies, Protozoan - blood
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
blood
Cat Diseases
Cat Diseases - diagnosis
Cat Diseases - parasitology
Cats
diagnosis
Dog Diseases
Dog Diseases - diagnosis
Dog Diseases - parasitology
Dogs
False Negative Reactions
Fluorescent Antibody Technique, Indirect
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
immunoglobulin G
Immunoglobulin G - blood
Immunology
Invertebrates
Medical Microbiology
Microbiology
Original Paper
parasitology
Sensitivity and Specificity
Toxoplasma
Toxoplasma - immunology
Toxoplasma gondii
Toxoplasmosis, Animal
Toxoplasmosis, Animal - diagnosis
Title Comparison of indirect fluorescent antibody test and modified agglutination test for detecting Toxoplasma gondii immunoglobulin G antibodies in dog and cat
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https://www.ncbi.nlm.nih.gov/pubmed/19221795
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https://www.proquest.com/docview/67294728
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