Modeling and Optimizing a New Culture Medium for In Vitro Rooting of G×N15 Prunus Rootstock using Artificial Neural Network-Genetic Algorithm

• Published in: Scientific reports Vol. 8; no. 1; pp. 9977 - 18
• Main Authors: Arab, Mohammad Mehdi, Yadollahi, Abbas, Eftekhari, Maliheh, Ahmadi, Hamed, Akbari, Mohammad, Khorami, Saadat Sarikhani
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 02.07.2018; Nature Publishing Group
• Subjects: 13/106; 631/449; 631/61; Algorithms; Calcium; Culture media; Genetic algorithms; Humanities and Social Sciences; Indole-3-butyric acid; multidisciplinary; Neural networks; Plant growth; Potassium; Prunus; Science; Science (multidisciplinary); Thiamine
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-27858-4

The main aim of the present investigation is modeling and optimization of a new culture medium for in vitro rooting of G×N15 rootstock using an artificial neural network-genetic algorithm (ANN-GA). Six experiments for assessing different media culture, various concentrations of Indole – 3- butyric acid, different concentrations of Thiamine and Fe-EDDHA were designed. The effects of five ionic macronutrients (NH 4 + , NO 3 − , Ca 2+ , K + and Cl − ) on five growth parameters [root number (RN), root length (RL), root percentage (R%), fresh (FW) and dry weight (DW)] were evaluated using the ANN-GA method. The R 2 correlation values of 0.88, 0.88, 0.98, 0.94 and 0.87 between observed and predicted values were acquired for all five growth parameters, respectively. The ANN-GA results indicated that among the input variables, K + (7.6) and NH4 + (4.4), K + (7.7) and Ca 2+ (2.8), K + (36.7) and NH 4 + (4.3), K + (14.7) and NH 4 + (4.4) and K + (7.6) and NH 4 + (4.3) had the highest values of variable sensitivity ratio (VSR) in the data set, for RN, RL, R%, FW and DW, respectively. ANN-GA optimized LS medium for G×N15 rooting contained optimized amounts of 1 mg L −1 IBA, 100, 150, or 200 mg L −1 Fe-EDDHA and 1.6 mg L −1 Thiamine. The efficiency of the optimized culture media was compared to other standard media for Prunus rooting and the results indicated that the optimized medium is more efficient than the others.