Effects of Ecklonia cava Extract on Neuronal Damage and Apoptosis in PC-12 Cells against Oxidative Stress

• Published in: Journal of microbiology and biotechnology Vol. 31; no. 4; pp. 584 - 591
• Main Authors: Shin, Yong Sub, Kim, Kwan Joong, Park, Hyein, Lee, Mi-Gi, Cho, Sueungmok, Choi, Soo-Im, Heo, Ho Jin, Kim, Dae-Ok, Kim, Gun-Hee
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Society for Microbiology and Biotechnology 28.04.2021; 한국미생물·생명공학회
• Subjects: Animals; Antioxidants - pharmacology; Apoptosis - drug effects; bcl-2-Associated X Protein; Benzofurans - pharmacology; Biological Products - pharmacology; Caspase 3; Neurons - drug effects; Oxidative Stress - drug effects; PC12 Cells; Phaeophyceae - chemistry; Rats; Research article; Seaweed; 생물학; seaweed; Ecklonia cava; phlorotannin-rich extract; oxidative stress; Dieckol
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.2012.12013

Marine algae (seaweed) encompass numerous groups of multicellular organisms with various shapes, sizes, and colors, and serve as important sources of natural bioactive substances. The brown alga Kjellman, an edible seaweed, contains many bioactives such as phlorotannins and fucoidans. Here, we evaluated the antioxidative, neuroprotective, and anti-apoptotic effects of extract (ECE), phlorotannin-rich extract (ECPE), and the phlorotannin dieckol on neuronal PC-12 cells. The antioxidant capacities of ECPE and ECE were 1,711.5 and 1,050.4 mg vitamin C equivalents/g in the ABTS assay and 704.0 and 474.6 mg vitamin C equivalents/g in the DPPH assay, respectively. The dieckol content of ECPE (58.99 mg/g) was approximately 60% higher than that of ECE (36.97 mg/g). Treatment of PC-12 cells with ECPE and ECE increased cell viability in a dose-dependent manner. Intracellular oxidative stress in PC-12 cells due to ECPE and ECE decreased dose-independently by up to 63% and 47%, respectively, compared with the stress control (323%). ECPE reduced the production of the pro-apoptotic proteins Bax and caspase-3 more effectively than ECE. Early and late apoptosis in PC-12 cells were more effectively decreased by ECPE than ECE treatments. From the results obtained in this study, we concluded that ECPE, which is rich in phlorotannins, including the marker compound dieckol, may be applied to the development of functional materials for improving cognition and memory.