High Mitochondrial DNA Copy Number Is a Protective Factor From Vision Loss in Heteroplasmic Leber's Hereditary Optic Neuropathy (LHON)

Leber's hereditary optic neuropathy (LHON) is a mitochondrial disease that typically causes bilateral blindness in young men. It is characterized by as yet undisclosed genetic and environmental factors affecting the incomplete penetrance. We identified 27 LHON subjects who possess heteroplasmic...

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Published inInvestigative ophthalmology & visual science Vol. 58; no. 4; p. 2193
Main Authors Bianco, Angelica, Bisceglia, Luigi, Russo, Luciana, Palese, Luigi L., D'Agruma, Leonardo, Emperador, Sonia, Montoya, Julio, Guerriero, Silvana, Petruzzella, Vittoria
Format Journal Article
LanguageEnglish
Published United States 01.04.2017
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ISSN1552-5783
0146-0404
1552-5783
DOI10.1167/iovs.16-20389

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Abstract Leber's hereditary optic neuropathy (LHON) is a mitochondrial disease that typically causes bilateral blindness in young men. It is characterized by as yet undisclosed genetic and environmental factors affecting the incomplete penetrance. We identified 27 LHON subjects who possess heteroplasmic primary LHON mutations. Mitochondrial DNA (mtDNA) copy number was evaluated. The presence of centrocecal scotoma, an edematous, hyperemic optic nerve head, and vascular tortuosity, as well as telangiectasia was recognized in affected subjects. We found higher cellular mtDNA content in peripheral blood cells of unaffected heteroplasmic mutation carriers with respect to the affected. The increase of cellular mtDNA content prevents complete loss of vision despite the presence of a heteroplasmic state of LHON primary mutation, suggesting that it is a key factor responsible for penetrance of LHON.
AbstractList Leber's hereditary optic neuropathy (LHON) is a mitochondrial disease that typically causes bilateral blindness in young men. It is characterized by as yet undisclosed genetic and environmental factors affecting the incomplete penetrance. We identified 27 LHON subjects who possess heteroplasmic primary LHON mutations. Mitochondrial DNA (mtDNA) copy number was evaluated. The presence of centrocecal scotoma, an edematous, hyperemic optic nerve head, and vascular tortuosity, as well as telangiectasia was recognized in affected subjects. We found higher cellular mtDNA content in peripheral blood cells of unaffected heteroplasmic mutation carriers with respect to the affected. The increase of cellular mtDNA content prevents complete loss of vision despite the presence of a heteroplasmic state of LHON primary mutation, suggesting that it is a key factor responsible for penetrance of LHON.
Leber's hereditary optic neuropathy (LHON) is a mitochondrial disease that typically causes bilateral blindness in young men. It is characterized by as yet undisclosed genetic and environmental factors affecting the incomplete penetrance.PURPOSELeber's hereditary optic neuropathy (LHON) is a mitochondrial disease that typically causes bilateral blindness in young men. It is characterized by as yet undisclosed genetic and environmental factors affecting the incomplete penetrance.We identified 27 LHON subjects who possess heteroplasmic primary LHON mutations. Mitochondrial DNA (mtDNA) copy number was evaluated.METHODSWe identified 27 LHON subjects who possess heteroplasmic primary LHON mutations. Mitochondrial DNA (mtDNA) copy number was evaluated.The presence of centrocecal scotoma, an edematous, hyperemic optic nerve head, and vascular tortuosity, as well as telangiectasia was recognized in affected subjects. We found higher cellular mtDNA content in peripheral blood cells of unaffected heteroplasmic mutation carriers with respect to the affected.RESULTSThe presence of centrocecal scotoma, an edematous, hyperemic optic nerve head, and vascular tortuosity, as well as telangiectasia was recognized in affected subjects. We found higher cellular mtDNA content in peripheral blood cells of unaffected heteroplasmic mutation carriers with respect to the affected.The increase of cellular mtDNA content prevents complete loss of vision despite the presence of a heteroplasmic state of LHON primary mutation, suggesting that it is a key factor responsible for penetrance of LHON.CONCLUSIONSThe increase of cellular mtDNA content prevents complete loss of vision despite the presence of a heteroplasmic state of LHON primary mutation, suggesting that it is a key factor responsible for penetrance of LHON.
Author Bisceglia, Luigi
Montoya, Julio
Bianco, Angelica
Petruzzella, Vittoria
Emperador, Sonia
Guerriero, Silvana
Palese, Luigi L.
D'Agruma, Leonardo
Russo, Luciana
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References 28820924 - Invest Ophthalmol Vis Sci. 2017 Aug 1;58(10 ):4077
29346492 - Invest Ophthalmol Vis Sci. 2018 Jan 1;59(1):331
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Snippet Leber's hereditary optic neuropathy (LHON) is a mitochondrial disease that typically causes bilateral blindness in young men. It is characterized by as yet...
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StartPage 2193
SubjectTerms Antioxidants - therapeutic use
Blindness - prevention & control
DNA Copy Number Variations
DNA, Mitochondrial - genetics
Female
Genes, Mitochondrial - genetics
Humans
Male
Mitochondria - genetics
Mitochondrial Diseases - diagnosis
Mitochondrial Diseases - drug therapy
Mitochondrial Diseases - genetics
Mutation
Optic Atrophy, Hereditary, Leber - diagnosis
Optic Atrophy, Hereditary, Leber - drug therapy
Optic Atrophy, Hereditary, Leber - genetics
Pedigree
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Ubiquinone - analogs & derivatives
Ubiquinone - therapeutic use
Visual Acuity
Title High Mitochondrial DNA Copy Number Is a Protective Factor From Vision Loss in Heteroplasmic Leber's Hereditary Optic Neuropathy (LHON)
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