Determination of Endogenous Peptides with in Vitro ACE Inhibitory Activity in Normotensive Human Plasma by the Fluorometric HPLC Method

• Published in: Bioscience, biotechnology, and biochemistry Vol. 61; no. 6; pp. 1052 - 1054
• Main Authors: Matsui, Toshiro, Matsufuji, Hiroshi, Kawasaki, Terukazu, Osajima, Yutaka
• Format: Journal Article
• Language: English
• Published: Tokyo Taylor & Francis 01.06.1997; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: ACE; Adult; Analytical, structural and metabolic biochemistry; Angiotensin II - blood; Angiotensin II - chemistry; Angiotensin II - metabolism; Angiotensin III - blood; Angiotensin III - chemistry; Angiotensin III - metabolism; angiotensin metabolites; Angiotensin-Converting Enzyme Inhibitors - blood; Angiotensin-Converting Enzyme Inhibitors - chemistry; Angiotensin-Converting Enzyme Inhibitors - metabolism; Biological and medical sciences; biotechnology; Blood Pressure - physiology; Chromatography, High Pressure Liquid; enzyme inhibitors; Enzymes and enzyme inhibitors; fluorometry; Fundamental and applied biological sciences. Psychology; HPLC; Humans; Hydrolases; In Vitro Techniques; Indicators and Reagents - chemistry; Male; males; metabolites; Naphthalenes - chemistry; peptides; Peptides - blood; peptidyl-dipeptidase A; Radioimmunoassay; Spectrometry, Fluorescence; Supine Position; Human; Biological fluid; Fluorescence detector; Peptides; Enzyme; Metabolite; HPLC chromatography; Enzyme inhibitor; Blood plasma; Peptidases; Peptidyl-dipeptidase A; Angiotensin; Hydrolases; Peptidyl-dipeptidases; ACE inhibitor; Quantitative analysis
• ISSN: 0916-8451; 1347-6947; 1347-6947
• DOI: 10.1271/bbb.61.1052

An in vitro degradation test of angiotensin (ANG) II or III in normotensive supine human plasma from 9 healthy male subjects confirmed the production of smaller ANG metabolites with angiotensin I-converting enzyme inhibitory activity. These metabolites were identified as ANG (3-8), ANG (4-8), ANG (5-8), and ANG (3-4), whose respective peptide concentrations were determined by our proposed naphthalene-2, 3-dialdehyde (NDA)-HPLC method to be 64 ±9, 39 ±5, 176 ± 22, and 197 ± 35 fmol/ml of plasma.