DNA is as effective as protein at inducing antibody in fish

• Published in: Vaccine Vol. 17; no. 7; pp. 965 - 972
• Main Authors: Kanellos, Theophanis, Sylvester, Ian D, Howard, Colin R, Russell, Peter H
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 26.02.1999; Elsevier
• Subjects: Animals; Antibodies, Viral - biosynthesis; Antibodies, Viral - blood; Antibody Affinity; avidity; beta-Galactosidase - administration & dosage; beta-Galactosidase - genetics; beta-Galactosidase - immunology; Biological and medical sciences; Brackish; Carassius auratus; DNA vaccine; Dose-Response Relationship, Immunologic; Fish; Freshwater; Fundamental and applied biological sciences. Psychology; Goldfish - immunology; Human cytomegalovirus; Humans; Injections, Intramuscular - veterinary; Injections, Intraperitoneal - veterinary; Marine; Microbiology; Muscle, Skeletal - immunology; Pisces; Plasmids - administration & dosage; Plasmids - immunology; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, DNA - administration & dosage; Vaccines, DNA - genetics; Vaccines, DNA - immunology; Virology; DNA vaccine; avidity; Fish; Intraperitoneal administration; Recombinant DNA; Mixed vaccine; Genetic vaccine; Route of administration; Intramuscular administration; Bacterin; Property formulation relationship; Vertebrata; Carassius auratus; Immunogenicity; Pisces; Immunological adjuvant; Pisciculture; Humoral immunity
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(98)00312-0

Antiviral vaccines are needed for fish. 50 μg plasmid DNA in saline by the intramuscular route and 10 μg β-gal protein in a commercial oil adjuvant by the peritoneal route induced serum antibody of the same titre and avidity in goldfish. The DNA expressed β-gal under control of the immediate early promoter/enhancer gene of human cytomegalovirus. Commercial bacterin vaccines are administered to fish by the intraperitoneal route with oil and this route for DNA induced only 2-fold less antibody than DNA by the intramuscular route. Bacterin vaccines and antiviral plasmid DNA could therefore be co-injected into the peritoneum of fish in an oil adjuvant as a single dose.