Knock-in of Enhanced Green Fluorescent Protein or/and Human Fibroblast Growth Factor 2 Gene into β-Casein Gene Locus in the Porcine Fibroblasts to Produce Therapeutic Protein
Transgenic animals have become important tools for the production of therapeutic proteins in the domestic animal. Production efficiencies of transgenic animals by conventional methods as microinjection and retrovirus vector methods are low, and the foreign gene expression levels are also low because...
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| Published in | Animal bioscience Vol. 27; no. 11; pp. 1644 - 1651 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Korea (South)
Asian - Australasian Association of Animal Production Societies
01.11.2014
Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 아세아·태평양축산학회 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1011-2367 1976-5517 2765-0189 1976-5517 2765-0235 |
| DOI | 10.5713/ajas.2014.14222 |
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| Abstract | Transgenic animals have become important tools for the production of therapeutic proteins in the domestic animal. Production efficiencies of transgenic animals by conventional methods as microinjection and retrovirus vector methods are low, and the foreign gene expression levels are also low because of their random integration in the host genome. In this study, we investigated the homologous recombination on the porcine β-casein gene locus using a knock-in vector for the β-casein gene locus. We developed the knock-in vector on the porcine β-casein gene locus and isolated knock-in fibroblast for nuclear transfer. The knock-in vector consisted of the neomycin resistance gene (neo) as a positive selectable marker gene, diphtheria toxin-A gene as negative selection marker, and 5' arm and 3' arm from the porcine β-casein gene. The secretion of enhanced green fluorescent protein (EGFP) was more easily detected in the cell culture media than it was by western blot analysis of cell extract of the HC11 mouse mammary epithelial cells transfected with EGFP knock-in vector. These results indicated that a knock-in system using β-casein gene induced high expression of transgene by the gene regulatory sequence of endogenous β-casein gene. These fibroblasts may be used to produce transgenic pigs for the production of therapeutic proteins via the mammary glands. |
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| AbstractList | Transgenic animals have become important tools for the production of therapeutic proteins in the domestic animal. Production efficiencies of transgenic animals by conventional methods as microinjection and retrovirus vector methods are low, and the foreign gene expression levels are also low because of their random integration in the host genome. In this study, we investigated the homologous recombination on the porcine β-casein gene locus using a knock-in vector for the β-casein gene locus. We developed the knock-in vector on the porcine β-casein gene locus and isolated knock-in fibroblast for nuclear transfer. The knock-in vector consisted of the neomycin resistance gene (neo) as a positive selectable marker gene, diphtheria toxin-A gene as negative selection marker, and 5′ arm and 3′ arm from the porcine β-casein gene. The secretion of enhanced green fluorescent protein (EGFP) was more easily detected in the cell culture media than it was by western blot analysis of cell extract of the HC11 mouse mammary epithelial cells transfected with EGFP knock-in vector. These results indicated that a knock-in system using β-casein gene induced high expression of transgene by the gene regulatory sequence of endogenous β-casein gene. These fibroblasts may be used to produce transgenic pigs for the production of therapeutic proteins via the mammary glands. KCI Citation Count: 1 Transgenic animals have become important tools for the production of therapeutic proteins in the domestic animal. Production efficiencies of transgenic animals by conventional methods as microinjection and retrovirus vector methods are low, and the foreign gene expression levels are also low because of their random integration in the host genome. In this study, we investigated the homologous recombination on the porcine β-casein gene locus using a knock-in vector for the β-casein gene locus. We developed the knock-in vector on the porcine β-casein gene locus and isolated knock-in fibroblast for nuclear transfer. The knock-in vector consisted of the neomycin resistance gene (neo) as a positive selectable marker gene, diphtheria toxin-A gene as negative selection marker, and 5' arm and 3' arm from the porcine β-casein gene. The secretion of enhanced green fluorescent protein (EGFP) was more easily detected in the cell culture media than it was by western blot analysis of cell extract of the HC11 mouse mammary epithelial cells transfected with EGFP knock-in vector. These results indicated that a knock-in system using β-casein gene induced high expression of transgene by the gene regulatory sequence of endogenous β-casein gene. These fibroblasts may be used to produce transgenic pigs for the production of therapeutic proteins via the mammary glands. Transgenic animals have become important tools for the production of therapeutic proteins in the domestic animal. Production efficiencies of transgenic animals by conventional methods as microinjection and retrovirus vector methods are low, and the foreign gene expression levels are also low because of their random integration in the host genome. In this study, we investigated the homologous recombination on the porcine β-casein gene locus using a knock-in vector for the β-casein gene locus. We developed the knock-in vector on the porcine β-casein gene locus and isolated knock-in fibroblast for nuclear transfer. The knock-in vector consisted of the neomycin resistance gene (neo) as a positive selectable marker gene, diphtheria toxin-A gene as negative selection marker, and 5' arm and 3' arm from the porcine β-casein gene. The secretion of enhanced green fluorescent protein (EGFP) was more easily detected in the cell culture media than it was by western blot analysis of cell extract of the HC11 mouse mammary epithelial cells transfected with EGFP knock-in vector. These results indicated that a knock-in system using β-casein gene induced high expression of transgene by the gene regulatory sequence of endogenous β-casein gene. These fibroblasts may be used to produce transgenic pigs for the production of therapeutic proteins via the mammary glands. (Key Words: Knock-in, Homologous Recombination, Therapeutic Proteins, Porcine β-casein Gene) Transgenic animals have become important tools for the production of therapeutic proteins in the domestic animal. Production efficiencies of transgenic animals by conventional methods as microinjection and retrovirus vector methods are low, and the foreign gene expression levels are also low because of their random integration in the host genome. In this study, we investigated the homologous recombination on the porcine β-casein gene locus using a knock-in vector for the β-casein gene locus. We developed the knock-in vector on the porcine β-casein gene locus and isolated knock-in fibroblast for nuclear transfer. The knock-in vector consisted of the neomycin resistance gene (neo) as a positive selectable marker gene, diphtheria toxin-A gene as negative selection marker, and 5' arm and 3' arm from the porcine β-casein gene. The secretion of enhanced green fluorescent protein (EGFP) was more easily detected in the cell culture media than it was by western blot analysis of cell extract of the HC11 mouse mammary epithelial cells transfected with EGFP knock-in vector. These results indicated that a knock-in system using β-casein gene induced high expression of transgene by the gene regulatory sequence of endogenous β-casein gene. These fibroblasts may be used to produce transgenic pigs for the production of therapeutic proteins via the mammary glands.Transgenic animals have become important tools for the production of therapeutic proteins in the domestic animal. Production efficiencies of transgenic animals by conventional methods as microinjection and retrovirus vector methods are low, and the foreign gene expression levels are also low because of their random integration in the host genome. In this study, we investigated the homologous recombination on the porcine β-casein gene locus using a knock-in vector for the β-casein gene locus. We developed the knock-in vector on the porcine β-casein gene locus and isolated knock-in fibroblast for nuclear transfer. The knock-in vector consisted of the neomycin resistance gene (neo) as a positive selectable marker gene, diphtheria toxin-A gene as negative selection marker, and 5' arm and 3' arm from the porcine β-casein gene. The secretion of enhanced green fluorescent protein (EGFP) was more easily detected in the cell culture media than it was by western blot analysis of cell extract of the HC11 mouse mammary epithelial cells transfected with EGFP knock-in vector. These results indicated that a knock-in system using β-casein gene induced high expression of transgene by the gene regulatory sequence of endogenous β-casein gene. These fibroblasts may be used to produce transgenic pigs for the production of therapeutic proteins via the mammary glands. |
| Audience | Academic |
| Author | Kim, Yeong Ji Kim, Ji Woo Jeong, Young-Hee Lee, Sang Mi Lee, Ji-Hye Kang, Man-Jong Kim, Se Eun Moon, Seung Ju Kim, Keun-Jung Kim, Min-Kyu |
| AuthorAffiliation | 1 Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25358326$$D View this record in MEDLINE/PubMed https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001927576$$DAccess content in National Research Foundation of Korea (NRF) |
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| Cites_doi | 10.1016/j.theriogenology.2010.11.001 10.1023/A:1008934912555 10.5713/ajas.2012.12146 10.1023/A:1008974616402 10.1016/j.cimid.2007.11.005 10.1016/S0093-691X(99)00244-7 10.1089/15204559950020076 10.1073/pnas.91.13.6138 10.1038/300611a0 10.1016/j.theriogenology.2006.09.034 10.1186/1477-7827-1-103 10.1093/hmg/9.3.353 10.1023/A:1018723712996 10.1111/j.1469-445X.2000.02110.x 10.1073/pnas.86.1.104 10.1023/A:1008914020843 10.1126/science.278.5346.2130 10.1360/yc-006-1513 10.1074/jbc.272.29.17972 10.1038/385810a0 10.1126/science.6272397 10.1038/35016604 10.1530/rep.0.1260001 10.1038/315680a0 10.1002/mrd.20595 10.1172/JCI2673 10.5713/ajas.2011.11240 |
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| Keywords | Homologous Recombination Porcine β-casein Gene Knock-in Therapeutic Proteins |
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| References | E1DMBP_2014_v27n11_1644_019 (E1DMBP_2014_v27n11_1644_021) 2004; 20 E1DMBP_2014_v27n11_1644_014 E1DMBP_2014_v27n11_1644_013 E1DMBP_2014_v27n11_1644_012 E1DMBP_2014_v27n11_1644_011 E1DMBP_2014_v27n11_1644_018 E1DMBP_2014_v27n11_1644_017 E1DMBP_2014_v27n11_1644_016 E1DMBP_2014_v27n11_1644_015 E1DMBP_2014_v27n11_1644_020 E1DMBP_2014_v27n11_1644_009 E1DMBP_2014_v27n11_1644_008 E1DMBP_2014_v27n11_1644_003 E1DMBP_2014_v27n11_1644_025 E1DMBP_2014_v27n11_1644_002 E1DMBP_2014_v27n11_1644_024 E1DMBP_2014_v27n11_1644_001 E1DMBP_2014_v27n11_1644_023 E1DMBP_2014_v27n11_1644_007 E1DMBP_2014_v27n11_1644_029 E1DMBP_2014_v27n11_1644_006 E1DMBP_2014_v27n11_1644_028 E1DMBP_2014_v27n11_1644_005 E1DMBP_2014_v27n11_1644_027 E1DMBP_2014_v27n11_1644_004 E1DMBP_2014_v27n11_1644_026 E1DMBP_2014_v27n11_1644_010 (E1DMBP_2014_v27n11_1644_022) 2005; 32 10655544 - Hum Mol Genet. 2000 Feb 12;9(3):353-61 11131009 - Transgenic Res. 2000;9(4-5):305-20 11131006 - Transgenic Res. 2000;9(4-5):263-75 17138536 - Yi Chuan. 2006 Dec;28(12):1513-9 10478491 - Transgenic Res. 1999 Jun;8(3):215-21 9039911 - Nature. 1997 Feb 27;385(6619):810-3 11187957 - Exp Physiol. 2000 Nov;85(6):615-25 6272397 - Science. 1981 Dec 11;214(4526):1244-6 8016126 - Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):6138-42 15971606 - Sheng Wu Gong Cheng Xue Bao. 2004 May;20(3):361-5 16998796 - Mol Reprod Dev. 2007 Apr;74(4):428-34 21196043 - Theriogenology. 2011 Mar 15;75(5):933-9 9405350 - Science. 1997 Dec 19;278(5346):2130-3 25049505 - Asian-Australas J Anim Sci. 2012 Oct;25(10):1473-80 16011027 - Yi Chuan Xue Bao. 2005 Apr;32(4):366-71 9649566 - J Clin Invest. 1998 Jul 1;102(1):130-5 6958982 - Nature. 1982 Dec 16;300(5893):611-5 14614774 - Reprod Biol Endocrinol. 2003 Nov 13;1:103 25049581 - Asian-Australas J Anim Sci. 2012 Mar;25(3):421-7 17070901 - Theriogenology. 2007 Jan 1;67(1):127-33 9218423 - J Biol Chem. 1997 Jul 18;272(29):17972-80 10890449 - Nature. 2000 Jun 29;405(6790):1066-9 12814342 - Reproduction. 2003 Jul;126(1):1-11 18243312 - Comp Immunol Microbiol Infect Dis. 2009 Mar;32(2):107-21 10735066 - Theriogenology. 2000 Jan 1;53(1):105-16 3892305 - Nature. 1985 Jun 20-26;315(6021):680-3 10819519 - J Mammary Gland Biol Neoplasia. 1998 Jul;3(3):337-50 2643093 - Proc Natl Acad Sci U S A. 1989 Jan;86(1):104-8 16218828 - Cloning. 1999;1(1):25-46 |
| References_xml | – ident: E1DMBP_2014_v27n11_1644_001 doi: 10.1016/j.theriogenology.2010.11.001 – ident: E1DMBP_2014_v27n11_1644_010 doi: 10.1023/A:1008934912555 – ident: E1DMBP_2014_v27n11_1644_012 doi: 10.5713/ajas.2012.12146 – ident: E1DMBP_2014_v27n11_1644_004 doi: 10.1023/A:1008974616402 – ident: E1DMBP_2014_v27n11_1644_011 doi: 10.1016/j.cimid.2007.11.005 – ident: E1DMBP_2014_v27n11_1644_018 doi: 10.1016/S0093-691X(99)00244-7 – ident: E1DMBP_2014_v27n11_1644_002 doi: 10.1089/15204559950020076 – ident: E1DMBP_2014_v27n11_1644_013 doi: 10.1073/pnas.91.13.6138 – ident: E1DMBP_2014_v27n11_1644_017 doi: 10.1038/300611a0 – ident: E1DMBP_2014_v27n11_1644_019 doi: 10.1016/j.theriogenology.2006.09.034 – ident: E1DMBP_2014_v27n11_1644_026 doi: 10.1186/1477-7827-1-103 – ident: E1DMBP_2014_v27n11_1644_008 doi: 10.1093/hmg/9.3.353 – ident: E1DMBP_2014_v27n11_1644_003 doi: 10.1023/A:1018723712996 – ident: E1DMBP_2014_v27n11_1644_028 doi: 10.1111/j.1469-445X.2000.02110.x – ident: E1DMBP_2014_v27n11_1644_006 doi: 10.1073/pnas.86.1.104 – ident: E1DMBP_2014_v27n11_1644_029 doi: 10.1023/A:1008914020843 – volume: 32 start-page: 366 year: 2005 ident: E1DMBP_2014_v27n11_1644_022 publication-title: Yi. Chuan. Xue. Bao. – ident: E1DMBP_2014_v27n11_1644_020 doi: 10.1126/science.278.5346.2130 – ident: E1DMBP_2014_v27n11_1644_015 doi: 10.1360/yc-006-1513 – ident: E1DMBP_2014_v27n11_1644_024 doi: 10.1074/jbc.272.29.17972 – ident: E1DMBP_2014_v27n11_1644_027 doi: 10.1038/385810a0 – ident: E1DMBP_2014_v27n11_1644_007 doi: 10.1126/science.6272397 – volume: 20 start-page: 361 year: 2004 ident: E1DMBP_2014_v27n11_1644_021 publication-title: Sheng. Wu. Gong. Cheng. Xue. Bao. – ident: E1DMBP_2014_v27n11_1644_016 doi: 10.1038/35016604 – ident: E1DMBP_2014_v27n11_1644_005 doi: 10.1530/rep.0.1260001 – ident: E1DMBP_2014_v27n11_1644_009 doi: 10.1038/315680a0 – ident: E1DMBP_2014_v27n11_1644_023 doi: 10.1002/mrd.20595 – ident: E1DMBP_2014_v27n11_1644_025 doi: 10.1172/JCI2673 – ident: E1DMBP_2014_v27n11_1644_014 doi: 10.5713/ajas.2011.11240 – reference: 3892305 - Nature. 1985 Jun 20-26;315(6021):680-3 – reference: 25049505 - Asian-Australas J Anim Sci. 2012 Oct;25(10):1473-80 – reference: 12814342 - Reproduction. 2003 Jul;126(1):1-11 – reference: 11131009 - Transgenic Res. 2000;9(4-5):305-20 – reference: 9039911 - Nature. 1997 Feb 27;385(6619):810-3 – reference: 18243312 - Comp Immunol Microbiol Infect Dis. 2009 Mar;32(2):107-21 – reference: 25049581 - Asian-Australas J Anim Sci. 2012 Mar;25(3):421-7 – reference: 16218828 - Cloning. 1999;1(1):25-46 – reference: 15971606 - Sheng Wu Gong Cheng Xue Bao. 2004 May;20(3):361-5 – reference: 17138536 - Yi Chuan. 2006 Dec;28(12):1513-9 – reference: 16011027 - Yi Chuan Xue Bao. 2005 Apr;32(4):366-71 – reference: 10735066 - Theriogenology. 2000 Jan 1;53(1):105-16 – reference: 21196043 - Theriogenology. 2011 Mar 15;75(5):933-9 – reference: 9405350 - Science. 1997 Dec 19;278(5346):2130-3 – reference: 8016126 - Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):6138-42 – reference: 10890449 - Nature. 2000 Jun 29;405(6790):1066-9 – reference: 9649566 - J Clin Invest. 1998 Jul 1;102(1):130-5 – reference: 6958982 - Nature. 1982 Dec 16;300(5893):611-5 – reference: 9218423 - J Biol Chem. 1997 Jul 18;272(29):17972-80 – reference: 6272397 - Science. 1981 Dec 11;214(4526):1244-6 – reference: 10819519 - J Mammary Gland Biol Neoplasia. 1998 Jul;3(3):337-50 – reference: 11187957 - Exp Physiol. 2000 Nov;85(6):615-25 – reference: 10478491 - Transgenic Res. 1999 Jun;8(3):215-21 – reference: 17070901 - Theriogenology. 2007 Jan 1;67(1):127-33 – reference: 11131006 - Transgenic Res. 2000;9(4-5):263-75 – reference: 10655544 - Hum Mol Genet. 2000 Feb 12;9(3):353-61 – reference: 14614774 - Reprod Biol Endocrinol. 2003 Nov 13;1:103 – reference: 16998796 - Mol Reprod Dev. 2007 Apr;74(4):428-34 – reference: 2643093 - Proc Natl Acad Sci U S A. 1989 Jan;86(1):104-8 |
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| SubjectTerms | Binding proteins Casein Cattle Fibroblast growth factors Genetic aspects Health aspects 축산학 |
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| Title | Knock-in of Enhanced Green Fluorescent Protein or/and Human Fibroblast Growth Factor 2 Gene into β-Casein Gene Locus in the Porcine Fibroblasts to Produce Therapeutic Protein |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/25358326 https://www.proquest.com/docview/1619320376 https://pubmed.ncbi.nlm.nih.gov/PMC4213711 https://www.animbiosci.org/upload/pdf/ajas-27-11-1644.pdf https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001927576 |
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| ispartofPNX | Animal Bioscience, 2014, 27(11), , pp.1644-1651 |
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