Generation of a rhesus macaque induced pluripotent stem cell line (riPSC05) under feeder-free conditions

We generated and characterized a rhesus macaque induced pluripotent stem cell (iPSC) line using induced reprogramming of fibroblasts isolated from a rhesus macaque fetus. The fibroblasts were expanded and then reprogrammed using non-integrating Sendai virus technology. This line is available as riPS...

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Published inStem cell research Vol. 73; p. 103241
Main Authors Lara, Mary Jasmine D., Wamaitha, Sissy E., Arabpour, Auriana, Hennebold, Jon D., Clark, Amander T., Sosa, Enrique
Format Journal Article
LanguageEnglish
Published England Elsevier 01.12.2023
Subjects
Animals
Cell Differentiation
Cellular Reprogramming
Fibroblasts - metabolism
Induced Pluripotent Stem Cells - metabolism
Karyotyping
Macaca mulatta
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ISSN1873-5061
1876-7753
1876-7753
DOI10.1016/j.scr.2023.103241

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Summary:We generated and characterized a rhesus macaque induced pluripotent stem cell (iPSC) line using induced reprogramming of fibroblasts isolated from a rhesus macaque fetus. The fibroblasts were expanded and then reprogrammed using non-integrating Sendai virus technology. This line is available as riPSC05. The authenticity of riPSC05 was confirmed through the expression of pluripotent and self-renewal markers, in vitro-directed differentiation towards three germ layers (ectoderm, mesoderm, and endoderm), karyotyping, and STR analysis.
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ISSN:1873-5061
1876-7753
1876-7753
DOI:10.1016/j.scr.2023.103241