phenix.mr_rosetta: molecular replacement and model rebuilding with Phenix and Rosetta

The combination of algorithms from the structure-modeling field with those of crystallographic structure determination can broaden the range of templates that are useful for structure determination by the method of molecular replacement. Automated tools in phenix.mr_rosetta simplify the application...

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Published inJournal of structural and functional genomics Vol. 13; no. 2; pp. 81 - 90
Main Authors Terwilliger, Thomas C., DiMaio, Frank, Read, Randy J., Baker, David, Bunkóczi, Gábor, Adams, Paul D., Grosse-Kunstleve, Ralf W., Afonine, Pavel V., Echols, Nathaniel
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Netherlands 01.06.2012
Springer Nature B.V
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Online AccessGet full text
ISSN1345-711X
1570-0267
1570-0267
DOI10.1007/s10969-012-9129-3

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Abstract The combination of algorithms from the structure-modeling field with those of crystallographic structure determination can broaden the range of templates that are useful for structure determination by the method of molecular replacement. Automated tools in phenix.mr_rosetta simplify the application of these combined approaches by integrating Phenix crystallographic algorithms and Rosetta structure-modeling algorithms and by systematically generating and evaluating models with a combination of these methods. The phenix.mr_rosetta algorithms can be used to automatically determine challenging structures. The approaches used in phenix.mr_rosetta are described along with examples that show roles that structure-modeling can play in molecular replacement.
AbstractList The combination of algorithms from the structure-modeling field with those of crystallographic structure determination can broaden the range of templates that are useful for structure determination by the method of molecular replacement. Automated tools in phenix.mr_rosetta simplify the application of these combined approaches by integrating Phenix crystallographic algorithms and Rosetta structure-modeling algorithms and by systematically generating and evaluating models with a combination of these methods. The phenix.mr_rosetta algorithms can be used to automatically determine challenging structures. The approaches used in phenix.mr_rosetta are described along with examples that show roles that structure-modeling can play in molecular replacement.
Issue Title: Special Issue: International Conference on Structural Genomics, 2011 The combination of algorithms from the structure-modeling field with those of crystallographic structure determination can broaden the range of templates that are useful for structure determination by the method of molecular replacement. Automated tools in phenix.mr_rosetta simplify the application of these combined approaches by integrating Phenix crystallographic algorithms and Rosetta structure-modeling algorithms and by systematically generating and evaluating models with a combination of these methods. The phenix.mr_rosetta algorithms can be used to automatically determine challenging structures. The approaches used in phenix.mr_rosetta are described along with examples that show roles that structure-modeling can play in molecular replacement.[PUBLICATION ABSTRACT]
The combination of algorithms from the structure-modeling field with those of crystallographic structure determination can broaden the range of templates that are useful for structure determination by the method of molecular replacement. Automated tools in phenix.mr_rosetta simplify the application of these combined approaches by integrating Phenix crystallographic algorithms and Rosetta structure-modeling algorithms and by systematically generating and evaluating models with a combination of these methods. The phenix.mr_rosetta algorithms can be used to automatically determine challenging structures. The approaches used in phenix.mr_rosetta are described along with examples that show roles that structure-modeling can play in molecular replacement.
The combination of algorithms from the structure-modeling field with those of crystallographic structure determination can broaden the range of templates that are useful for structure determination by the method of molecular replacement. Automated tools in phenix.mr_rosetta simplify the application of these combined approaches by integrating Phenix crystallographic algorithms and Rosetta structure-modeling algorithms and by systematically generating and evaluating models with a combination of these methods. The phenix.mr_rosetta algorithms can be used to automatically determine challenging structures. The approaches used in phenix.mr_rosetta are described along with examples that show roles that structure-modeling can play in molecular replacement.The combination of algorithms from the structure-modeling field with those of crystallographic structure determination can broaden the range of templates that are useful for structure determination by the method of molecular replacement. Automated tools in phenix.mr_rosetta simplify the application of these combined approaches by integrating Phenix crystallographic algorithms and Rosetta structure-modeling algorithms and by systematically generating and evaluating models with a combination of these methods. The phenix.mr_rosetta algorithms can be used to automatically determine challenging structures. The approaches used in phenix.mr_rosetta are described along with examples that show roles that structure-modeling can play in molecular replacement.
Author Baker, David
Read, Randy J.
Grosse-Kunstleve, Ralf W.
Bunkóczi, Gábor
Adams, Paul D.
DiMaio, Frank
Afonine, Pavel V.
Echols, Nathaniel
Terwilliger, Thomas C.
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  givenname: Thomas C.
  surname: Terwilliger
  fullname: Terwilliger, Thomas C.
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  organization: Los Alamos Institutes and BioScience Division, Los Alamos National Laboratory
– sequence: 2
  givenname: Frank
  surname: DiMaio
  fullname: DiMaio, Frank
  organization: Department of Biochemistry, University of Washington
– sequence: 3
  givenname: Randy J.
  surname: Read
  fullname: Read, Randy J.
  organization: Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge
– sequence: 4
  givenname: David
  surname: Baker
  fullname: Baker, David
  organization: Department of Biochemistry, University of Washington
– sequence: 5
  givenname: Gábor
  surname: Bunkóczi
  fullname: Bunkóczi, Gábor
  organization: Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge
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  givenname: Paul D.
  surname: Adams
  fullname: Adams, Paul D.
  organization: Lawrence Berkeley National Laboratory
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  givenname: Ralf W.
  surname: Grosse-Kunstleve
  fullname: Grosse-Kunstleve, Ralf W.
  organization: Lawrence Berkeley National Laboratory
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  givenname: Pavel V.
  surname: Afonine
  fullname: Afonine, Pavel V.
  organization: Lawrence Berkeley National Laboratory
– sequence: 9
  givenname: Nathaniel
  surname: Echols
  fullname: Echols, Nathaniel
  organization: Lawrence Berkeley National Laboratory
BackLink https://www.ncbi.nlm.nih.gov/pubmed/22418934$$D View this record in MEDLINE/PubMed
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Issue 2
Keywords Macromolecular crystallography
Molecular replacement
Automation
Language English
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PublicationTitle Journal of structural and functional genomics
PublicationTitleAbbrev J Struct Funct Genomics
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Springer Nature B.V
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References PerrakisAMorrisRLamzinVSAutomated protein model building combined with iterative structure refinementNature Struct Biol19996458463
MaoBGuanRMontelioneGTImproved technologies now routinely provide protein NMR structures useful for molecular replacementStructure2011197577662164584910.1016/j.str.2011.04.0051:CAS:528:DC%2BC3MXntFGgurk%3D
LangerGCohenSXLamzinVSPerrakisAAutomated macromolecular model building for X-ray crystallography using ARP/wARP version 7Nat Protoc20083117111791860022210.1038/nprot.2008.911:CAS:528:DC%2BD1cXotFaku7s%3D
ChenYWDodsonEJKleywegtGJDoes NMR mean “not for molecular replacement”? using NMR-based search models to solve protein crystal structuresStructure2000821322010.1016/S0969-2126(00)00524-41:CAS:528:DC%2BD3cXhsFGqs7k%3D
SchröderGLevittMBrüngerATSuper-resolution biomolecular crystallography with low-resolution dataNature2010464121812222037600610.1038/nature08892
QianBRamanSDasRBradleyPMcCoyAJReadRJBakerDHigh resolution structure prediction and the crystallographic phase problemNature20074502592641793444710.1038/nature062491:CAS:528:DC%2BD2sXht1yntrnO
Emsley P, Lohkamp B, Scott WG, Cowtan K (2010) Features and development of Coot. Acat Cryst D66:486–501
OldfieldTJAutomated tracing of electron density maps of proteinsActa Cryst2003D594834911:CAS:528:DC%2BD3sXhtlaitbY%3D
DiMaioFTykaMDBakerMLChiuWBakerDRefinement of protein structures into low-resolution density maps using RosettaJ Mol Biol20093921811901959633910.1016/j.jmb.2009.07.0081:CAS:528:DC%2BD1MXhtVKrsrnO
McCoyAJGrosse-KunstleveRWAdamsPDWinnMDStoroniLCReadRJPhaser crystallographic softwareJ Appl Cryst20074065867410.1107/S00218898070212061:CAS:528:DC%2BD2sXnslWqsLk%3D
Afonine PV, Grosse-Kunstleve RW, Adams PD (2005) The Phenix refinement framework. CCP4 newsl. 42, contribution 8
KeeganRMLongFFazioVJWinnMDMurshudovGNVaginAAEvaluating the solution from MrBUMP and BALBESActa Cryst2011D673133231:CAS:528:DC%2BC3MXktFWqtLc%3D
OldfieldTJPattern-recognition methods to identify secondary structure within X-ray crystallographic electron-density mapsActa Cryst2002D584874931:CAS:528:DC%2BD38XhtlGntLs%3D
KideraAGoNNormal mode refinement—crystallographic refinement of protein dynamic structure. 1. Theory and test by simulated diffraction dataJ Mol Biol1992225457475159363010.1016/0022-2836(92)90932-A1:CAS:528:DyaK38Xks1Kjs70%3D
BunkocziGReadRJImprovement of molecular-replacement models with SculptorActa Cryst2010D67303312
CowtanKThe Buccaneer software for automated model buildingActa Cryst2006D62100210111:CAS:528:DC%2BD28XosVSjtrc%3D
ChivianDKimDEMalmstromLBradleyPRobertsonTMurphyPStraussCEMBonneauRRohlCABakerDAutomated prediction of CASP-5 structures using the Robetta serverProteins200353Suppl 65245331457934210.1002/prot.105291:CAS:528:DC%2BD3sXptVygtbw%3D
SchwarzenbacherRGodzikAGrzechnikSKJaroszewskiLThe importance of alignment accuracy for molecular replacementActa Cryst D2004601229123610.1107/S0907444904010145
AdamsPDAfoninePVBunkocziGChenVBDavisIWEcholsNHeaddJJHungLWKapralGJGrosse-KunstleveRWMcCoyAJMoriartyNWOeffnerRReadRJRichardsonDCRichardsonJSTerwilligerTCZwartPHPHENIX: a comprehensive Python-based system for macromolecular structure solutionActa Cryst2010D662132211:CAS:528:DC%2BC3cXhs1Sisbc%3D
DasRBakerDProspects for de novo phasing with de novo protein modelsActa Cryst2009D651691751:CAS:528:DC%2BD1MXhtVWhtL0%3D
IoergerTRSacchettiniJCTEXTAL system: artificial intelligence techniques for automated protein model buildingMethods Enzymol20033742442701469637710.1016/S0076-6879(03)74012-91:CAS:528:DC%2BD2cXotlygug%3D%3D
LongFVaginAYoungPMurshudovGNBALBES: a molecular replacement pipelineActa Cryst2008D641251321:CAS:528:DC%2BD2sXhsVGjt7fJ
DelarueMDealing with structural variability in molecular replacement and crystallographic refinement through normal-mode analysisActa Cryst2008D6440481:CAS:528:DC%2BD2sXhsVGjt7nK
TerwilligerTCGrosse-KunstleveRWAfoninePVMoriartyNWZwartPHHungLWReadRJAdamsPDIterative model building, structure refinement and density modification with the PHENIX AutoBuild wizardActa Cryst D200864616910.1107/S090744490705024X
RossmannMGThe molecular replacement method1972New YorkGodon & Breach
DiMaioFKondrashovDABittoESoniABingmanCAPhillipsGNJrShavlikJWBioinformatics200723285128581793385510.1093/bioinformatics/btm4801:CAS:528:DC%2BD2sXhtlaqsL3I
TerwilligerTCRapid model-building of alpha-helices in electron density mapsActa Cryst2010D662682751:CAS:528:DC%2BC3cXisValt7o%3D
TerwilligerTCUsing prime-and-switch phasing to reduce model bias in molecular replacementActa Cryst2004D60214421491:CAS:528:DC%2BD2cXhtVars73J
RamelotTARamanSKuzinAPXiaoRMaLCActonTBHuntJFMontelioneGTBakerDKennedyMAImproving NMR protein structure quality by Rosetta refinement: a molecular replacement studyProteins2009751471671881679910.1002/prot.222291:CAS:528:DC%2BD1MXisV2isr0%3D
LiMDiMaioFZhouDGustchinaALubkowskiJDauterZBakerDWlodawerACrystal structure of XMRV protease differs from the structures of other retropepsinsNat Struct Mol Biol2011182272292125832310.1038/nsmb.19641:CAS:528:DC%2BC3MXps1agsw%3D%3D
BermanHMWestbrookJFengZGillilandGBhatTNWeissigHShindyalovINBournePEThe protein data bankNucleic Acids Res2000282352421059223510.1093/nar/28.1.2351:CAS:528:DC%2BD3cXhvVKjt7w%3D
ReadRJPushing the boundaries of molecular replacement with maximum likelihoodActa Cryst D2001571373138210.1107/S09074449010124711:STN:280:DC%2BD3MrhvFehtg%3D%3D
DiMaioFTerwilligerTCReadRJWlodawerAOberdorferGWagnerUValkovEAlonAFassDAxelrodHLDasDVorobievSMIwaiHPokkuluriPRBakerDImproving molecular replacement by density and energy guided protein structure optimizationNature20114735405432153258910.1038/nature099641:CAS:528:DC%2BC3MXlsVGltr8%3D
SödingJProtein homology detection by HMM–HMM comparisonBioinformatics2005219519601553160310.1093/bioinformatics/bti125
LyskowskiAOeemigJSJaakonenARommiKDiMaioFZhouDKajanderTBakerDWlodawerAGoldmanAIwaïHCloning, expression, purification, crystallization and preliminary X-ray diffraction data of the Pyrococcus horikoshii RadA inteinActa Cryst2011F676236261:CAS:528:DC%2BC3MXlvFGiu7k%3D
LevittDGA new software routine that automates the fitting of protein X-ray crystallographic electron density mapsActa Cryst2001D57101310191:CAS:528:DC%2BD3MXksVyrsLg%3D
EvansPMcCoyAAn introduction to molecular replacementActa Cryst2008D641101:CAS:528:DC%2BD2sXhsVGjt7jL
BakerMLJuTChiuWIdentification of secondary structure elements in intermediate resolution density mapsStructure2007157191722352810.1016/j.str.2006.11.0081:CAS:528:DC%2BD2sXmtVentw%3D%3D
KovalevskyAYLiuFLeshchenkoSGhoshAKLouisJMHarrisonRWWeberITUltra-high resolution crystal structure of HIV-1 protease mutant reveals two binding sites for clinical inhibitor TMC114J Mol Biol20063631611731696213610.1016/j.jmb.2006.08.0071:CAS:528:DC%2BD28XpvFSitLY%3D
Stokes-ReesISlizPProtein structure determination by exhaustive search of Protein Data Bank derived databasesProc Natl Acad Sci USA201010721476214812109830610.1073/pnas.10120951071:CAS:528:DC%2BC3cXhs1WisL7P
TerwilligerTCStatistical density modification with non-crystallographic symmetryActa Cryst2002D58208220861:CAS:528:DC%2BD3sXkslymtw%3D%3D
RoversiPBlancEVonrheinCEvansGBricogneGRefinement of severely incomplete structures with maximum likelihood in BUSTER-TNTActa Cryst2000D56131613231:CAS:528:DC%2BD3cXnsFart7s%3D
TC Terwilliger (9129_CR36) 2002; D58
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F DiMaio (9129_CR28) 2011; 473
B Qian (9129_CR29) 2007; 450
F DiMaio (9129_CR37) 2009; 392
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M Delarue (9129_CR10) 2008; D64
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R Schwarzenbacher (9129_CR7) 2004; 60
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TC Terwilliger (9129_CR16) 2004; D60
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D Chivian (9129_CR34) 2003; 53
G Schröder (9129_CR41) 2010; 464
G Langer (9129_CR17) 2008; 3
TC Terwilliger (9129_CR19) 2008; 64
9129_CR35
MG Rossmann (9129_CR1) 1972
TJ Oldfield (9129_CR26) 2003; D59
9129_CR42
F DiMaio (9129_CR22) 2007; 23
J Söding (9129_CR33) 2005; 21
K Cowtan (9129_CR21) 2006; D62
M Li (9129_CR39) 2011; 18
P Roversi (9129_CR15) 2000; D56
A Lyskowski (9129_CR38) 2011; F67
YW Chen (9129_CR4) 2000; 8
A Kidera (9129_CR11) 1992; 225
F Long (9129_CR12) 2008; D64
ML Baker (9129_CR20) 2007; 15
P Evans (9129_CR2) 2008; D64
RJ Read (9129_CR9) 2001; 57
DG Levitt (9129_CR24) 2001; D57
TC Terwilliger (9129_CR27) 2010; D66
G Bunkoczi (9129_CR5) 2010; D67
I Stokes-Rees (9129_CR13) 2010; 107
TR Ioerger (9129_CR23) 2003; 374
References_xml – reference: BermanHMWestbrookJFengZGillilandGBhatTNWeissigHShindyalovINBournePEThe protein data bankNucleic Acids Res2000282352421059223510.1093/nar/28.1.2351:CAS:528:DC%2BD3cXhvVKjt7w%3D
– reference: KideraAGoNNormal mode refinement—crystallographic refinement of protein dynamic structure. 1. Theory and test by simulated diffraction dataJ Mol Biol1992225457475159363010.1016/0022-2836(92)90932-A1:CAS:528:DyaK38Xks1Kjs70%3D
– reference: KovalevskyAYLiuFLeshchenkoSGhoshAKLouisJMHarrisonRWWeberITUltra-high resolution crystal structure of HIV-1 protease mutant reveals two binding sites for clinical inhibitor TMC114J Mol Biol20063631611731696213610.1016/j.jmb.2006.08.0071:CAS:528:DC%2BD28XpvFSitLY%3D
– reference: SchröderGLevittMBrüngerATSuper-resolution biomolecular crystallography with low-resolution dataNature2010464121812222037600610.1038/nature08892
– reference: OldfieldTJAutomated tracing of electron density maps of proteinsActa Cryst2003D594834911:CAS:528:DC%2BD3sXhtlaitbY%3D
– reference: Emsley P, Lohkamp B, Scott WG, Cowtan K (2010) Features and development of Coot. Acat Cryst D66:486–501
– reference: DiMaioFTerwilligerTCReadRJWlodawerAOberdorferGWagnerUValkovEAlonAFassDAxelrodHLDasDVorobievSMIwaiHPokkuluriPRBakerDImproving molecular replacement by density and energy guided protein structure optimizationNature20114735405432153258910.1038/nature099641:CAS:528:DC%2BC3MXlsVGltr8%3D
– reference: BunkocziGReadRJImprovement of molecular-replacement models with SculptorActa Cryst2010D67303312
– reference: TerwilligerTCUsing prime-and-switch phasing to reduce model bias in molecular replacementActa Cryst2004D60214421491:CAS:528:DC%2BD2cXhtVars73J
– reference: RossmannMGThe molecular replacement method1972New YorkGodon & Breach
– reference: LongFVaginAYoungPMurshudovGNBALBES: a molecular replacement pipelineActa Cryst2008D641251321:CAS:528:DC%2BD2sXhsVGjt7fJ
– reference: LangerGCohenSXLamzinVSPerrakisAAutomated macromolecular model building for X-ray crystallography using ARP/wARP version 7Nat Protoc20083117111791860022210.1038/nprot.2008.911:CAS:528:DC%2BD1cXotFaku7s%3D
– reference: LyskowskiAOeemigJSJaakonenARommiKDiMaioFZhouDKajanderTBakerDWlodawerAGoldmanAIwaïHCloning, expression, purification, crystallization and preliminary X-ray diffraction data of the Pyrococcus horikoshii RadA inteinActa Cryst2011F676236261:CAS:528:DC%2BC3MXlvFGiu7k%3D
– reference: McCoyAJGrosse-KunstleveRWAdamsPDWinnMDStoroniLCReadRJPhaser crystallographic softwareJ Appl Cryst20074065867410.1107/S00218898070212061:CAS:528:DC%2BD2sXnslWqsLk%3D
– reference: SchwarzenbacherRGodzikAGrzechnikSKJaroszewskiLThe importance of alignment accuracy for molecular replacementActa Cryst D2004601229123610.1107/S0907444904010145
– reference: CowtanKThe Buccaneer software for automated model buildingActa Cryst2006D62100210111:CAS:528:DC%2BD28XosVSjtrc%3D
– reference: PerrakisAMorrisRLamzinVSAutomated protein model building combined with iterative structure refinementNature Struct Biol19996458463
– reference: DelarueMDealing with structural variability in molecular replacement and crystallographic refinement through normal-mode analysisActa Cryst2008D6440481:CAS:528:DC%2BD2sXhsVGjt7nK
– reference: ChenYWDodsonEJKleywegtGJDoes NMR mean “not for molecular replacement”? using NMR-based search models to solve protein crystal structuresStructure2000821322010.1016/S0969-2126(00)00524-41:CAS:528:DC%2BD3cXhsFGqs7k%3D
– reference: DiMaioFKondrashovDABittoESoniABingmanCAPhillipsGNJrShavlikJWBioinformatics200723285128581793385510.1093/bioinformatics/btm4801:CAS:528:DC%2BD2sXhtlaqsL3I
– reference: LiMDiMaioFZhouDGustchinaALubkowskiJDauterZBakerDWlodawerACrystal structure of XMRV protease differs from the structures of other retropepsinsNat Struct Mol Biol2011182272292125832310.1038/nsmb.19641:CAS:528:DC%2BC3MXps1agsw%3D%3D
– reference: EvansPMcCoyAAn introduction to molecular replacementActa Cryst2008D641101:CAS:528:DC%2BD2sXhsVGjt7jL
– reference: RamelotTARamanSKuzinAPXiaoRMaLCActonTBHuntJFMontelioneGTBakerDKennedyMAImproving NMR protein structure quality by Rosetta refinement: a molecular replacement studyProteins2009751471671881679910.1002/prot.222291:CAS:528:DC%2BD1MXisV2isr0%3D
– reference: OldfieldTJPattern-recognition methods to identify secondary structure within X-ray crystallographic electron-density mapsActa Cryst2002D584874931:CAS:528:DC%2BD38XhtlGntLs%3D
– reference: Afonine PV, Grosse-Kunstleve RW, Adams PD (2005) The Phenix refinement framework. CCP4 newsl. 42, contribution 8
– reference: ChivianDKimDEMalmstromLBradleyPRobertsonTMurphyPStraussCEMBonneauRRohlCABakerDAutomated prediction of CASP-5 structures using the Robetta serverProteins200353Suppl 65245331457934210.1002/prot.105291:CAS:528:DC%2BD3sXptVygtbw%3D
– reference: ReadRJPushing the boundaries of molecular replacement with maximum likelihoodActa Cryst D2001571373138210.1107/S09074449010124711:STN:280:DC%2BD3MrhvFehtg%3D%3D
– reference: QianBRamanSDasRBradleyPMcCoyAJReadRJBakerDHigh resolution structure prediction and the crystallographic phase problemNature20074502592641793444710.1038/nature062491:CAS:528:DC%2BD2sXht1yntrnO
– reference: MaoBGuanRMontelioneGTImproved technologies now routinely provide protein NMR structures useful for molecular replacementStructure2011197577662164584910.1016/j.str.2011.04.0051:CAS:528:DC%2BC3MXntFGgurk%3D
– reference: TerwilligerTCStatistical density modification with non-crystallographic symmetryActa Cryst2002D58208220861:CAS:528:DC%2BD3sXkslymtw%3D%3D
– reference: LevittDGA new software routine that automates the fitting of protein X-ray crystallographic electron density mapsActa Cryst2001D57101310191:CAS:528:DC%2BD3MXksVyrsLg%3D
– reference: Stokes-ReesISlizPProtein structure determination by exhaustive search of Protein Data Bank derived databasesProc Natl Acad Sci USA201010721476214812109830610.1073/pnas.10120951071:CAS:528:DC%2BC3cXhs1WisL7P
– reference: DiMaioFTykaMDBakerMLChiuWBakerDRefinement of protein structures into low-resolution density maps using RosettaJ Mol Biol20093921811901959633910.1016/j.jmb.2009.07.0081:CAS:528:DC%2BD1MXhtVKrsrnO
– reference: RoversiPBlancEVonrheinCEvansGBricogneGRefinement of severely incomplete structures with maximum likelihood in BUSTER-TNTActa Cryst2000D56131613231:CAS:528:DC%2BD3cXnsFart7s%3D
– reference: DasRBakerDProspects for de novo phasing with de novo protein modelsActa Cryst2009D651691751:CAS:528:DC%2BD1MXhtVWhtL0%3D
– reference: SödingJProtein homology detection by HMM–HMM comparisonBioinformatics2005219519601553160310.1093/bioinformatics/bti125
– reference: KeeganRMLongFFazioVJWinnMDMurshudovGNVaginAAEvaluating the solution from MrBUMP and BALBESActa Cryst2011D673133231:CAS:528:DC%2BC3MXktFWqtLc%3D
– reference: TerwilligerTCGrosse-KunstleveRWAfoninePVMoriartyNWZwartPHHungLWReadRJAdamsPDIterative model building, structure refinement and density modification with the PHENIX AutoBuild wizardActa Cryst D200864616910.1107/S090744490705024X
– reference: IoergerTRSacchettiniJCTEXTAL system: artificial intelligence techniques for automated protein model buildingMethods Enzymol20033742442701469637710.1016/S0076-6879(03)74012-91:CAS:528:DC%2BD2cXotlygug%3D%3D
– reference: BakerMLJuTChiuWIdentification of secondary structure elements in intermediate resolution density mapsStructure2007157191722352810.1016/j.str.2006.11.0081:CAS:528:DC%2BD2sXmtVentw%3D%3D
– reference: TerwilligerTCRapid model-building of alpha-helices in electron density mapsActa Cryst2010D662682751:CAS:528:DC%2BC3cXisValt7o%3D
– reference: AdamsPDAfoninePVBunkocziGChenVBDavisIWEcholsNHeaddJJHungLWKapralGJGrosse-KunstleveRWMcCoyAJMoriartyNWOeffnerRReadRJRichardsonDCRichardsonJSTerwilligerTCZwartPHPHENIX: a comprehensive Python-based system for macromolecular structure solutionActa Cryst2010D662132211:CAS:528:DC%2BC3cXhs1Sisbc%3D
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Snippet The combination of algorithms from the structure-modeling field with those of crystallographic structure determination can broaden the range of templates that...
Issue Title: Special Issue: International Conference on Structural Genomics, 2011 The combination of algorithms from the structure-modeling field with those of...
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SubjectTerms Algorithms
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Title phenix.mr_rosetta: molecular replacement and model rebuilding with Phenix and Rosetta
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