A novel ELISA system for selective measurement of human adiponectin multimers by using proteases
Adiponectin, an antiatherogenic adipocyte-derived protein exists in human blood as multiple isoforms—trimeric low molecular weight (LMW), albumin-binding LMW (Alb-LMW), hexameric middle molecular weight (MMW), and high molecular weight (HMW) forms. We developed a novel ELISA system to detect total h...
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| Published in | Clinica chimica acta Vol. 372; no. 1; pp. 47 - 53 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
Elsevier B.V
01.10.2006
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 0009-8981 1873-3492 |
| DOI | 10.1016/j.cca.2006.03.014 |
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| Abstract | Adiponectin, an antiatherogenic adipocyte-derived protein exists in human blood as multiple isoforms—trimeric low molecular weight (LMW), albumin-binding LMW (Alb-LMW), hexameric middle molecular weight (MMW), and high molecular weight (HMW) forms. We developed a novel ELISA system to detect total human adiponectin and the selective level of each adiponectin multimer for investigating the distribution of these levels in human blood.
Two monoclonal antibodies that were raised against human adiponectin were used to construct a sandwich ELISA to measure adiponectin levels. Adiponectin multimers were selectively measured after sample pretreatment with two proteases that specifically digested the trimeric forms or both the hexameric and trimeric forms.
The ELISA had a dynamic range of 0.075–4.8 ng/ml. Intraassay variations (CV) were 5.3% (total adiponectin), 4.1% (MMW
+
HMW), and 3.3% (HMW). Comparison of the results of ELISA and quantitative western blot analysis of multimeric adiponectin in serum samples revealed good correlation (LMW
+
Alb-LMW,
r
=
0.873; MMW,
r
=
0.907; HMW,
r
=
0.950). Each of the three forms of adiponectin multimer levels closely correlated with total adiponectin levels in healthy subjects.
This ELISA system can be used to further investigate the physiological roles of human adiponectin multimers. |
|---|---|
| AbstractList | Adiponectin, an antiatherogenic adipocyte-derived protein exists in human blood as multiple isoforms—trimeric low molecular weight (LMW), albumin-binding LMW (Alb-LMW), hexameric middle molecular weight (MMW), and high molecular weight (HMW) forms. We developed a novel ELISA system to detect total human adiponectin and the selective level of each adiponectin multimer for investigating the distribution of these levels in human blood.
Two monoclonal antibodies that were raised against human adiponectin were used to construct a sandwich ELISA to measure adiponectin levels. Adiponectin multimers were selectively measured after sample pretreatment with two proteases that specifically digested the trimeric forms or both the hexameric and trimeric forms.
The ELISA had a dynamic range of 0.075–4.8 ng/ml. Intraassay variations (CV) were 5.3% (total adiponectin), 4.1% (MMW
+
HMW), and 3.3% (HMW). Comparison of the results of ELISA and quantitative western blot analysis of multimeric adiponectin in serum samples revealed good correlation (LMW
+
Alb-LMW,
r
=
0.873; MMW,
r
=
0.907; HMW,
r
=
0.950). Each of the three forms of adiponectin multimer levels closely correlated with total adiponectin levels in healthy subjects.
This ELISA system can be used to further investigate the physiological roles of human adiponectin multimers. Adiponectin, an antiatherogenic adipocyte-derived protein exists in human blood as multiple isoforms--trimeric low molecular weight (LMW), albumin-binding LMW (Alb-LMW), hexameric middle molecular weight (MMW), and high molecular weight (HMW) forms. We developed a novel ELISA system to detect total human adiponectin and the selective level of each adiponectin multimer for investigating the distribution of these levels in human blood.BACKGROUNDAdiponectin, an antiatherogenic adipocyte-derived protein exists in human blood as multiple isoforms--trimeric low molecular weight (LMW), albumin-binding LMW (Alb-LMW), hexameric middle molecular weight (MMW), and high molecular weight (HMW) forms. We developed a novel ELISA system to detect total human adiponectin and the selective level of each adiponectin multimer for investigating the distribution of these levels in human blood.Two monoclonal antibodies that were raised against human adiponectin were used to construct a sandwich ELISA to measure adiponectin levels. Adiponectin multimers were selectively measured after sample pretreatment with two proteases that specifically digested the trimeric forms or both the hexameric and trimeric forms.METHODSTwo monoclonal antibodies that were raised against human adiponectin were used to construct a sandwich ELISA to measure adiponectin levels. Adiponectin multimers were selectively measured after sample pretreatment with two proteases that specifically digested the trimeric forms or both the hexameric and trimeric forms.The ELISA had a dynamic range of 0.075-4.8 ng/ml. Intraassay variations (CV) were 5.3% (total adiponectin), 4.1% (MMW+HMW), and 3.3% (HMW). Comparison of the results of ELISA and quantitative western blot analysis of multimeric adiponectin in serum samples revealed good correlation (LMW+Alb-LMW, r=0.873; MMW, r=0.907; HMW, r=0.950). Each of the three forms of adiponectin multimer levels closely correlated with total adiponectin levels in healthy subjects.RESULTSThe ELISA had a dynamic range of 0.075-4.8 ng/ml. Intraassay variations (CV) were 5.3% (total adiponectin), 4.1% (MMW+HMW), and 3.3% (HMW). Comparison of the results of ELISA and quantitative western blot analysis of multimeric adiponectin in serum samples revealed good correlation (LMW+Alb-LMW, r=0.873; MMW, r=0.907; HMW, r=0.950). Each of the three forms of adiponectin multimer levels closely correlated with total adiponectin levels in healthy subjects.This ELISA system can be used to further investigate the physiological roles of human adiponectin multimers.CONCLUSIONSThis ELISA system can be used to further investigate the physiological roles of human adiponectin multimers. Adiponectin, an antiatherogenic adipocyte-derived protein exists in human blood as multiple isoforms--trimeric low molecular weight (LMW), albumin-binding LMW (Alb-LMW), hexameric middle molecular weight (MMW), and high molecular weight (HMW) forms. We developed a novel ELISA system to detect total human adiponectin and the selective level of each adiponectin multimer for investigating the distribution of these levels in human blood. Two monoclonal antibodies that were raised against human adiponectin were used to construct a sandwich ELISA to measure adiponectin levels. Adiponectin multimers were selectively measured after sample pretreatment with two proteases that specifically digested the trimeric forms or both the hexameric and trimeric forms. The ELISA had a dynamic range of 0.075-4.8 ng/ml. Intraassay variations (CV) were 5.3% (total adiponectin), 4.1% (MMW+HMW), and 3.3% (HMW). Comparison of the results of ELISA and quantitative western blot analysis of multimeric adiponectin in serum samples revealed good correlation (LMW+Alb-LMW, r=0.873; MMW, r=0.907; HMW, r=0.950). Each of the three forms of adiponectin multimer levels closely correlated with total adiponectin levels in healthy subjects. This ELISA system can be used to further investigate the physiological roles of human adiponectin multimers. |
| Author | Yago, Hirokazu Ebinuma, Hiroyuki Kadowaki, Takashi Yamauchi, Toshimasa Miyazaki, Osamu Hara, Kazuo |
| Author_xml | – sequence: 1 givenname: Hiroyuki surname: Ebinuma fullname: Ebinuma, Hiroyuki email: ebinuma@daiichichem.co.jp organization: Diagnostics Research Laboratories, Daiichi Pure Chemicals Co. Ltd., 3-3-1, Koyodai, Ryugasaki, Ibaraki 301-0852, Japan – sequence: 2 givenname: Osamu surname: Miyazaki fullname: Miyazaki, Osamu organization: Diagnostics Research Laboratories, Daiichi Pure Chemicals Co. Ltd., 3-3-1, Koyodai, Ryugasaki, Ibaraki 301-0852, Japan – sequence: 3 givenname: Hirokazu surname: Yago fullname: Yago, Hirokazu organization: Diagnostics Research Laboratories, Daiichi Pure Chemicals Co. Ltd., 3-3-1, Koyodai, Ryugasaki, Ibaraki 301-0852, Japan – sequence: 4 givenname: Kazuo surname: Hara fullname: Hara, Kazuo organization: Department of Clinical Bioinformatics, Graduate School of Medicine, University of Tokyo 113-8655, Japan – sequence: 5 givenname: Toshimasa surname: Yamauchi fullname: Yamauchi, Toshimasa organization: Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo 113-8655, Japan – sequence: 6 givenname: Takashi surname: Kadowaki fullname: Kadowaki, Takashi organization: Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo 113-8655, Japan |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/16697359$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1161/01.CIR.103.8.1057 10.1093/oxfordjournals.jbchem.a021483 10.2337/diabetes.53.6.1621 10.1016/S0140-6736(03)12255-6 10.1006/bbrc.1996.0587 10.1074/jbc.M311113200 10.1111/j.1749-6632.1964.tb14213.x 10.2337/diacare.26.7.2015 10.1074/jbc.271.18.10697 10.1161/01.RES.0000119921.86460.37 10.1074/jbc.M206083200 10.1210/jc.86.5.1930 10.1074/jbc.270.45.26746 10.1006/bbrc.1999.0255 10.1074/jbc.C200312200 10.1074/jbc.M309469200 10.1074/jbc.M300365200 10.1074/jbc.M207198200 |
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| References | Tsao, Murrey, Hug, Lee, Lodish (bib12) 2002; 277 Tsao, Tomas, Murrey (bib13) 2003; 278 Scherer, Williams, Fogliano, Baldini, Lodish (bib1) 1995; 270 Nakano, Tobe, Choi-Miura, Mazda, Tomita (bib4) 1996; 120 Davis (bib18) 1964; 121 Hu, Liang, Spiegelman (bib2) 1996; 271 Arita, Kihara, Ouchi (bib8) 1999; 257 Maeda, Okubo, Shimomura, Funahashi, Matsuzawa, Matsubara (bib3) 1996; 221 Waki, Yamauchi, Kamon (bib14) 2003; 278 Spranger, Kroke, Möhlig (bib6) 2002; 361 Tonelli, Li, Kishore (bib17) 2004; 53 Ouchi, Kihara, Arita (bib9) 2001; 103 Weyer, Funahashi, Tanaka (bib5) 2001; 86 Daimon, Yamaguchi, Oizumi (bib7) 2003; 26 Pajvani, Du, Combs (bib11) 2003; 278 Matsuda, Shimomura, Sata (bib10) 2002; 277 Pajvani, Hawkins, Combs (bib16) 2004; 279 Kobayashi, Ouchi, Kihara (bib15) 2004; 94 Waki (10.1016/j.cca.2006.03.014_bib14) 2003; 278 Maeda (10.1016/j.cca.2006.03.014_bib3) 1996; 221 Daimon (10.1016/j.cca.2006.03.014_bib7) 2003; 26 Pajvani (10.1016/j.cca.2006.03.014_bib11) 2003; 278 Arita (10.1016/j.cca.2006.03.014_bib8) 1999; 257 Tsao (10.1016/j.cca.2006.03.014_bib13) 2003; 278 Tonelli (10.1016/j.cca.2006.03.014_bib17) 2004; 53 Davis (10.1016/j.cca.2006.03.014_bib18) 1964; 121 Pajvani (10.1016/j.cca.2006.03.014_bib16) 2004; 279 Hu (10.1016/j.cca.2006.03.014_bib2) 1996; 271 Tsao (10.1016/j.cca.2006.03.014_bib12) 2002; 277 Nakano (10.1016/j.cca.2006.03.014_bib4) 1996; 120 Spranger (10.1016/j.cca.2006.03.014_bib6) 2002; 361 Matsuda (10.1016/j.cca.2006.03.014_bib10) 2002; 277 Scherer (10.1016/j.cca.2006.03.014_bib1) 1995; 270 Kobayashi (10.1016/j.cca.2006.03.014_bib15) 2004; 94 Ouchi (10.1016/j.cca.2006.03.014_bib9) 2001; 103 Weyer (10.1016/j.cca.2006.03.014_bib5) 2001; 86 |
| References_xml | – volume: 361 start-page: 226 year: 2002 end-page: 228 ident: bib6 article-title: Adiponectin and protection against type 2 diabetes mellitus publication-title: Lancet – volume: 103 start-page: 1057 year: 2001 end-page: 1063 ident: bib9 article-title: Adipocyte-derived plasma protein, adiponectin, suppresses lipid accumulation and class A scavenger receptor expression in human monocyte-derived macrophages publication-title: Circulation – volume: 278 start-page: 50810 year: 2003 end-page: 50817 ident: bib13 article-title: Role of disulfide bonds in Acrp30/adiponectin structure and signaling specificity. Different oligomers activate different signal transduction pathways publication-title: J Biol Chem – volume: 271 start-page: 10697 year: 1996 end-page: 10703 ident: bib2 article-title: AdipoQ is a novel adipose-specific gene dysregulated in obesity publication-title: J Biol Chem – volume: 277 start-page: 29359 year: 2002 end-page: 29362 ident: bib12 article-title: Oligomerization state-dependent activation of NF-kappa B signaling pathway by adipocyte complement-related protein of 30 kDa (Acrp30) publication-title: J Biol Chem – volume: 53 start-page: 1621 year: 2004 end-page: 1629 ident: bib17 article-title: Mechanisms of early insulin-sensitizing effects of thiazolidinediones in type 2 diabetes publication-title: Diabetes – volume: 86 start-page: 1930 year: 2001 end-page: 1935 ident: bib5 article-title: Hyperadiponectinemia in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia publication-title: J Clin Endocrinol Metab – volume: 279 start-page: 12152 year: 2004 end-page: 12162 ident: bib16 article-title: Complex distribution, not absolute amount of adiponectin, correlates with thiazolidinedione-mediated improvement in insulin sensitivity publication-title: J Biol Chem – volume: 257 start-page: 79 year: 1999 end-page: 83 ident: bib8 article-title: Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity publication-title: Biochem Biophys Res Commun – volume: 121 start-page: 404 year: 1964 end-page: 427 ident: bib18 article-title: Disc electrophoresis. II. Method and application to human serum proteins publication-title: Ann NY Acad Sci – volume: 270 start-page: 26746 year: 1995 end-page: 26749 ident: bib1 article-title: A novel serum protein similar to C1q, produced exclusively in adipocytes publication-title: J Biol Chem – volume: 221 start-page: 286 year: 1996 end-page: 296 ident: bib3 article-title: cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (AdiPose Most abundant Gene transcript 1) publication-title: Biochem Biophys Res Commun – volume: 278 start-page: 40352 year: 2003 end-page: 40363 ident: bib14 article-title: Impaired multimerization of human adiponectin mutants associated with diabetes. Molecular structure and multimer formation of adiponectin publication-title: J Biol Chem – volume: 94 start-page: e27 year: 2004 end-page: e31 ident: bib15 article-title: Selective suppression of endothelial cell apoptosis by the high molecular weight form of adiponectin publication-title: Circ Res – volume: 26 start-page: 2015 year: 2003 end-page: 2020 ident: bib7 article-title: Decreased serum levels of adiponectin are a risk factor for the progression to type 2 diabetes in the Japanese population: the Funagata study publication-title: Diabetes Care – volume: 277 start-page: 37487 year: 2002 end-page: 37491 ident: bib10 article-title: Role of adiponectin in preventing vascular stenosis—the missing link of adipo-vascular axis publication-title: J Biol Chem – volume: 278 start-page: 9073 year: 2003 end-page: 9085 ident: bib11 article-title: Structure–function studies of the adipocyte-secreted hormone Acrp30/adiponectin. Implications for metabolic regulation and bioactivity publication-title: J Biol Chem – volume: 120 start-page: 803 year: 1996 end-page: 812 ident: bib4 article-title: Isolation and characterization of GBP28, a novel gelatin-binding protein purified from human plasma publication-title: J Biochem (Tokyo) – volume: 103 start-page: 1057 year: 2001 ident: 10.1016/j.cca.2006.03.014_bib9 article-title: Adipocyte-derived plasma protein, adiponectin, suppresses lipid accumulation and class A scavenger receptor expression in human monocyte-derived macrophages publication-title: Circulation doi: 10.1161/01.CIR.103.8.1057 – volume: 120 start-page: 803 year: 1996 ident: 10.1016/j.cca.2006.03.014_bib4 article-title: Isolation and characterization of GBP28, a novel gelatin-binding protein purified from human plasma publication-title: J Biochem (Tokyo) doi: 10.1093/oxfordjournals.jbchem.a021483 – volume: 53 start-page: 1621 year: 2004 ident: 10.1016/j.cca.2006.03.014_bib17 article-title: Mechanisms of early insulin-sensitizing effects of thiazolidinediones in type 2 diabetes publication-title: Diabetes doi: 10.2337/diabetes.53.6.1621 – volume: 361 start-page: 226 year: 2002 ident: 10.1016/j.cca.2006.03.014_bib6 article-title: Adiponectin and protection against type 2 diabetes mellitus publication-title: Lancet doi: 10.1016/S0140-6736(03)12255-6 – volume: 221 start-page: 286 year: 1996 ident: 10.1016/j.cca.2006.03.014_bib3 article-title: cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (AdiPose Most abundant Gene transcript 1) publication-title: Biochem Biophys Res Commun doi: 10.1006/bbrc.1996.0587 – volume: 279 start-page: 12152 year: 2004 ident: 10.1016/j.cca.2006.03.014_bib16 article-title: Complex distribution, not absolute amount of adiponectin, correlates with thiazolidinedione-mediated improvement in insulin sensitivity publication-title: J Biol Chem doi: 10.1074/jbc.M311113200 – volume: 121 start-page: 404 year: 1964 ident: 10.1016/j.cca.2006.03.014_bib18 article-title: Disc electrophoresis. II. Method and application to human serum proteins publication-title: Ann NY Acad Sci doi: 10.1111/j.1749-6632.1964.tb14213.x – volume: 26 start-page: 2015 year: 2003 ident: 10.1016/j.cca.2006.03.014_bib7 article-title: Decreased serum levels of adiponectin are a risk factor for the progression to type 2 diabetes in the Japanese population: the Funagata study publication-title: Diabetes Care doi: 10.2337/diacare.26.7.2015 – volume: 271 start-page: 10697 year: 1996 ident: 10.1016/j.cca.2006.03.014_bib2 article-title: AdipoQ is a novel adipose-specific gene dysregulated in obesity publication-title: J Biol Chem doi: 10.1074/jbc.271.18.10697 – volume: 94 start-page: e27 year: 2004 ident: 10.1016/j.cca.2006.03.014_bib15 article-title: Selective suppression of endothelial cell apoptosis by the high molecular weight form of adiponectin publication-title: Circ Res doi: 10.1161/01.RES.0000119921.86460.37 – volume: 277 start-page: 37487 year: 2002 ident: 10.1016/j.cca.2006.03.014_bib10 article-title: Role of adiponectin in preventing vascular stenosis—the missing link of adipo-vascular axis publication-title: J Biol Chem doi: 10.1074/jbc.M206083200 – volume: 86 start-page: 1930 year: 2001 ident: 10.1016/j.cca.2006.03.014_bib5 article-title: Hyperadiponectinemia in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia publication-title: J Clin Endocrinol Metab doi: 10.1210/jc.86.5.1930 – volume: 270 start-page: 26746 year: 1995 ident: 10.1016/j.cca.2006.03.014_bib1 article-title: A novel serum protein similar to C1q, produced exclusively in adipocytes publication-title: J Biol Chem doi: 10.1074/jbc.270.45.26746 – volume: 257 start-page: 79 year: 1999 ident: 10.1016/j.cca.2006.03.014_bib8 article-title: Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity publication-title: Biochem Biophys Res Commun doi: 10.1006/bbrc.1999.0255 – volume: 277 start-page: 29359 year: 2002 ident: 10.1016/j.cca.2006.03.014_bib12 article-title: Oligomerization state-dependent activation of NF-kappa B signaling pathway by adipocyte complement-related protein of 30 kDa (Acrp30) publication-title: J Biol Chem doi: 10.1074/jbc.C200312200 – volume: 278 start-page: 50810 year: 2003 ident: 10.1016/j.cca.2006.03.014_bib13 article-title: Role of disulfide bonds in Acrp30/adiponectin structure and signaling specificity. Different oligomers activate different signal transduction pathways publication-title: J Biol Chem doi: 10.1074/jbc.M309469200 – volume: 278 start-page: 40352 year: 2003 ident: 10.1016/j.cca.2006.03.014_bib14 article-title: Impaired multimerization of human adiponectin mutants associated with diabetes. Molecular structure and multimer formation of adiponectin publication-title: J Biol Chem doi: 10.1074/jbc.M300365200 – volume: 278 start-page: 9073 year: 2003 ident: 10.1016/j.cca.2006.03.014_bib11 article-title: Structure–function studies of the adipocyte-secreted hormone Acrp30/adiponectin. Implications for metabolic regulation and bioactivity publication-title: J Biol Chem doi: 10.1074/jbc.M207198200 |
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| Snippet | Adiponectin, an antiatherogenic adipocyte-derived protein exists in human blood as multiple isoforms—trimeric low molecular weight (LMW), albumin-binding LMW... Adiponectin, an antiatherogenic adipocyte-derived protein exists in human blood as multiple isoforms--trimeric low molecular weight (LMW), albumin-binding LMW... |
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| SubjectTerms | Adiponectin Adiponectin - analysis Adiponectin - chemistry Adiponectin - immunology Antibodies, Monoclonal - immunology Biopolymers - analysis Blotting, Western ELISA Enzyme-Linked Immunosorbent Assay - methods Humans Monoclonal antibody Multimers Protease |
| Title | A novel ELISA system for selective measurement of human adiponectin multimers by using proteases |
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