Characterization of Composite Nano-Bioscaffolds Based on Collagen and Supercritical Fluids-Assisted Decellularized Fibrous Extracellular Matrix

Nano-bioscaffolds obtained from decellularized tissues have been employed in several medical applications. Nano-bioscaffolds could provide structural support for cell attachment and a suitable environment with sufficient porosity for cell growth and proliferation. In this study, a new combined metho...

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Published inPolymers Vol. 13; no. 24; p. 4326
Main Authors Huang, Ching-Cheng, Chen, Ying-Ju, Liu, Hsia-Wei
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 10.12.2021
MDPI
Subjects
Biocompatibility
Carbon dioxide
Cell cycle
Collagen
Extracellular matrix
Fourier transforms
Infrared radiation
Methods
Morphology
Penicillin
Scanning electron microscopy
Spectrum analysis
Supercritical fluids
Tendons
United States > US
China
Japan
Taiwan
decellularization
morphology
supercritical fluid
nano-bioscaffold
dermis
Online AccessGet full text
ISSN2073-4360
2073-4360
DOI10.3390/polym13244326

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Abstract Nano-bioscaffolds obtained from decellularized tissues have been employed in several medical applications. Nano-bioscaffolds could provide structural support for cell attachment and a suitable environment with sufficient porosity for cell growth and proliferation. In this study, a new combined method constitutes a decellularization protocol to remove the tissue and cellular molecules from porcine dermis for preparation of nano-bioscaffolds with fibrous extracellular matrix via pre- and post-treatment of supercritical fluids. The supercritical fluids-assisted nano-bioscaffolds were characterized by peptide identification, infrared spectrum of absorption, morphology, histological observations, DNA quantification, and hemocompatibility. Further, the resulting nano-bioscaffolds could be employed to obtain new cross-linked composite nano-bioscaffold containing collagen and acellular matrix.
AbstractList Nano-bioscaffolds obtained from decellularized tissues have been employed in several medical applications. Nano-bioscaffolds could provide structural support for cell attachment and a suitable environment with sufficient porosity for cell growth and proliferation. In this study, a new combined method constitutes a decellularization protocol to remove the tissue and cellular molecules from porcine dermis for preparation of nano-bioscaffolds with fibrous extracellular matrix via pre- and post-treatment of supercritical fluids. The supercritical fluids-assisted nano-bioscaffolds were characterized by peptide identification, infrared spectrum of absorption, morphology, histological observations, DNA quantification, and hemocompatibility. Further, the resulting nano-bioscaffolds could be employed to obtain new cross-linked composite nano-bioscaffold containing collagen and acellular matrix.
Nano-bioscaffolds obtained from decellularized tissues have been employed in several medical applications. Nano-bioscaffolds could provide structural support for cell attachment and a suitable environment with sufficient porosity for cell growth and proliferation. In this study, a new combined method constitutes a decellularization protocol to remove the tissue and cellular molecules from porcine dermis for preparation of nano-bioscaffolds with fibrous extracellular matrix via pre- and post-treatment of supercritical fluids. The supercritical fluids-assisted nano-bioscaffolds were characterized by peptide identification, infrared spectrum of absorption, morphology, histological observations, DNA quantification, and hemocompatibility. Further, the resulting nano-bioscaffolds could be employed to obtain new cross-linked composite nano-bioscaffold containing collagen and acellular matrix.Nano-bioscaffolds obtained from decellularized tissues have been employed in several medical applications. Nano-bioscaffolds could provide structural support for cell attachment and a suitable environment with sufficient porosity for cell growth and proliferation. In this study, a new combined method constitutes a decellularization protocol to remove the tissue and cellular molecules from porcine dermis for preparation of nano-bioscaffolds with fibrous extracellular matrix via pre- and post-treatment of supercritical fluids. The supercritical fluids-assisted nano-bioscaffolds were characterized by peptide identification, infrared spectrum of absorption, morphology, histological observations, DNA quantification, and hemocompatibility. Further, the resulting nano-bioscaffolds could be employed to obtain new cross-linked composite nano-bioscaffold containing collagen and acellular matrix.
Author Chen, Ying-Ju
Huang, Ching-Cheng
Liu, Hsia-Wei
AuthorAffiliation 2 Department Life Science, Fu Jen Catholic University, New Taipei City 242062, Taiwan
4 PARSD Biomedical Material Research Center, Taichung City 40749, Taiwan
3 Graduate Institute of Applied Science and Engineering, Fu Jen Catholic University, New Taipei City 242062, Taiwan
1 Department of Biomedical Engineering, Ming-Chuan University, Taoyuan City 32033, Taiwan; junas.tw@yahoo.com.tw (C.-C.H.); chyiju.tw@gmail.com (Y.-J.C.)
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Keywords decellularization
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nano-bioscaffold
dermis
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Snippet Nano-bioscaffolds obtained from decellularized tissues have been employed in several medical applications. Nano-bioscaffolds could provide structural support...
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SubjectTerms Biocompatibility
Carbon dioxide
Cell cycle
Collagen
Extracellular matrix
Fourier transforms
Infrared radiation
Methods
Morphology
Penicillin
Scanning electron microscopy
Spectrum analysis
Supercritical fluids
Tendons
Title Characterization of Composite Nano-Bioscaffolds Based on Collagen and Supercritical Fluids-Assisted Decellularized Fibrous Extracellular Matrix
URI https://www.ncbi.nlm.nih.gov/pubmed/34960876
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