Characterization of Composite Nano-Bioscaffolds Based on Collagen and Supercritical Fluids-Assisted Decellularized Fibrous Extracellular Matrix

• Published in: Polymers Vol. 13; no. 24; p. 4326
• Main Authors: Huang, Ching-Cheng, Chen, Ying-Ju, Liu, Hsia-Wei
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10.12.2021; MDPI
• Subjects: Biocompatibility; Carbon dioxide; Cell cycle; Collagen; Extracellular matrix; Fourier transforms; Infrared radiation; Methods; Morphology; Penicillin; Scanning electron microscopy; Spectrum analysis; Supercritical fluids; Tendons; United States > US; China; Japan; Taiwan; decellularization; morphology; supercritical fluid; nano-bioscaffold; dermis
• ISSN: 2073-4360; 2073-4360
• DOI: 10.3390/polym13244326

Nano-bioscaffolds obtained from decellularized tissues have been employed in several medical applications. Nano-bioscaffolds could provide structural support for cell attachment and a suitable environment with sufficient porosity for cell growth and proliferation. In this study, a new combined method constitutes a decellularization protocol to remove the tissue and cellular molecules from porcine dermis for preparation of nano-bioscaffolds with fibrous extracellular matrix via pre- and post-treatment of supercritical fluids. The supercritical fluids-assisted nano-bioscaffolds were characterized by peptide identification, infrared spectrum of absorption, morphology, histological observations, DNA quantification, and hemocompatibility. Further, the resulting nano-bioscaffolds could be employed to obtain new cross-linked composite nano-bioscaffold containing collagen and acellular matrix.